Regeneration and agrobacterium mediated transformation in sweet orange (Citrus sinensis L. Osbeck) cv. Mosambi
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Date
2008
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Publisher
CCSHAU
Abstract
The present study was carried out to develop an efficient
regeneration protocol for genetic transformation of sweet orange using
Agrobacterium mediated transformation. Direct regeneration of shoots,
roots and plant without intervention of callus from epicotyl segments
was observed on MS basal medium supplemented with various
cytokinins and auxins. The MS+BAP 1.0mg/l was observed to be the
best treatment for shoot proliferation in in vitro epicotyl explants. The
MS+BAP 1.0mg/l was found to be the most effective one for
morphogenic growth parameters like number of shoots and shoot
length. The number of shoots per explant ranged from 0.87 to 3.03. The
maximum length of shoot was 2.80 cm on the MS+BAP 1mg/l from in
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vitro epicotyl segments. Medium ½ MS+IBA 1.0mg/l was found to be
best for different aspects of root differentiation like number of days
required for root initiation (13.80), completion of rooting (25.60), per
cent rooting (100%) number of roots/planted (3.80), root length
(3.88cm) and percent survival of rooted plantlets (100%).
In transformation experiment, highest percent transient GUS
expression was observed on incubation of Agrobacterium for 15 minutes
and co-cultivated for 3 days on regeneration medium.
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Keywords
Fruits, Vegetative propagation, Auxins, Regeneration, Iba, Planting, Genetic processes, Biological phenomena, Bacteria, Confectionery