DEVELOPMENT OF MICROBIAL CONSORTIUM AND ITS EFFECT ON GREEN GRAM (Vigna radiata)
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Date
2022
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Dr.RPCAU, Pusa
Abstract
PGPR is a community of root – colonizing bacteria that found in the root of several plant species, and have the ability to maintain root health and promote nutrient uptake and environment stress tolerance which improve plant growth and commercial yield. In the present study a tota l of 19 PGPR-based bacterial isolates viz. six Bacillus spp. (BS1, BS2, BS3, BS4, BS5, BS6), four Azotobacter spp. (AZT1, AZT2, AZT3, AZT4), nine Rhizobium spp. (RZM1, RZM2, RZM3, RZM4, RZL1, RZL2, RZL3, RZL4, RZP1) were taken from the stock culture of the Microbiology department, CBS&H, Pusa. These PGPR isolates were tested for their ability to solubilize nutrients, specifically phosphorus, zinc, siderophore synthesis, and IAA production. Out of 19 bacterical isolates, 5 isolates i.e. AZT4, BS2, BS5, RZL1, RZM2 were found promising one and were further used for developing PGPR consortium. It was found that all the 5 promising one were compatible with each other. The developed consortium was screened for plant growth promoting characters and found the PGPR consortium showed PSI – 3.82, ZnSI – 3.45, siderophore production efficiency - 267 %, IAA – 122 μg mL-1. Further, a pot experiment was carried out to evaluate the effectiveness of PGPR consortium in combination with different levels of NPK on growth parameters (plant height, nodule number, nodule weight, no. of branches and chlorophyll content) of green gram ((Vigna mungo L.) and soil nutrient parameters (available Nitrogen, Phosphorus, Potash, Organic
Carbon, microbial load). All crop and soil nutrient parameters analyzed increased with application of PGPR consortium compared to the absolute control (without PGPR consortia and RDF). Crop and soil parameters were significantly influenced with the application of NPK fertilizer and PGPR consortia intervention in green gram crop and the maximum value of parameters was obtained in treatment containing PGPR consortia + 100% RDF followed by 75% RDF + Rhizobium as compared to 50 % RDF. At any level of fertilizer application, consortia mode always showed higher value than mono mode. Integration of microbial consortia with chemical fertilizers opens up a huge window for improving crop output while also improving nutrient use efficiency. Hence, in order to improve the growth and performance of the moong bean the developed consortium of Rhizobium + Bacillus + Azotobacter can be used as a (biofertilizer) for the soil of North Bihar state.