MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS AND DETECTION OF LEPTOSPIRA SEROVARS PREVALENT IN DOMESTIC ANIMALS IN WAYANAD DISTRICT
Loading...
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES POOKODE, WAYANAD
Abstract
Leptospirosis has been identified as re-emerged potentially zoonotic disease
with wide distribution in developing countries like India. In regions like Wayanad,
leptospirosis is underdiagnosed and prevalent serovars are unknown due to lack of
previous studies and due to lack of facilities for proper diagnosis. In this scenario, a
comprehensive study was conducted for identification of prevalent serovars and detection
of infection in domestic animals of Wayanad district by molecular methods. Total 71
samples including blood, serum, urine and tissue samples were collected from clinically
suspected cases of leptospirosis and from different regions of Wayanad district. All the
samples collected were subjected to total DNA extraction and PCR was carried out using
G1/G2 and lipL32 based primers. The test could detect amplicons in positive control but
no positive results were yielded by clinical samples.Out of total 62 serum samples tested
by MAT, the standard serological test for leptospirosis, 20 samples have given positive
result. The most predominant serovar identified was Pyrogenes which was followed by
Grippotyphosa, Bataviae, Icterohaemorrhageae, Javanica, Pomona, Australis and
Canicola. Mixed infections were also observed which included Pyrogenes and Bataviae,
Pyrogenes and Javanica, Grippotyphosa and Canicola, Pyrogenes and Australis,
Grippotyphosa and Javanica. The MAT titre of agglutinating serovars varied from 1:3200
to 1: 200. Highest MAT titre observed was 1:3200 which was against serovar Pyrogenes.