EVALUATION OF Typha angustata AND Prosopis juliflora AS WOUND HEALING AGENTS IN CATTLE-CLINICAL, BIOMECHANICAL AND HISTOLOGICAL STUDIES
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Date
1994
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AAU, Anand
Abstract
Each of 12 healthy crossbred male cow calves were subjected to ten excisional wounds, five on each side of thoracolumbar region to evaluate the wound healing property of Typha angustata, Prosopis juliflora and an ayurvedic ointment Swellnil , The wound healing was evaluated clinically, and by histological and biomechanical examination of excised healing tissue, collected at 7, 14 and 28 days. Granulation of tissues was evident on 4 to 6th day; contraction and epithelialization on 7 to 9th day and complete healing between 21 and 23 days. Extensive fibroblastic proliferation, high vascularity, non-significant acid phosphatase and alicaline phosphatase activities, intense mucopolysaccharide concentration and thin collagen fibres were observed in histological examirmtion at early stage of healing. At later stage, marked reduction in cellularity, vascularity and mucopolysaccharide concentration, increased acid phosphatase and alkaline phosphatase activities, matured collagen fibre bundles and elastic fibres were observed. Biomechanical study revealed increase in tensile strength with progress in healing, whereas extensibility increased on 14th day, but decreased on 28th day. No significant difference was observed between treatments as well as control in clinical, histological and biomechanical studies. In clinical trials conducted in 51 cases complete healing was observed between 8 and 22 days. The laboratory examination of the swabs collected periodically from the wounds showed presence of microorganisms upto 6 days in P. juliflora treated wounds and upto 9 days in T. angustata and Swellnil treated wounds. The microorganisms identified from the clinical wounds were Staphylococcus aureus (14), Staphylococcus (non-coagulase) (1), Streptococcus spp. (9), Escherichia coli (6), Proteus spp. (10), Corynebacterium pyogens (3)Klebsiella spp. (2), Pseudomonas spp. (3) and yeast (5). No distinct difference was observed in the healing period of wounds treated with three different ointments. Prom the present study it can be concluded that the T.angustata, P.juliflora and Swellnil ointments have no deleterious effect on wound healing. In clinical cases they help in normalization of the healing process.
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VETERINARY SURGERY AND RADIOLOGY, EVALUATION