Biochemical evaluation of bitter gourd genotypes in response to yellow mosaic virus disease
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
The study evaluated biochemical changes in resistant and susceptible bitter gourd plants; 4 cultivars and 11 advanced lines inoculated with viruliferous whiteflies for about 2 weeks for study of yellow mosaic virus disease (YMVD) caused by yellow mosaic virus (YMV). The disease severity was scored at 15, 30, 45, and 60 days of inoculation (DOI) and grouped the plants into highly resistant (Jhaar Karela, Punjab-15, and Punjab Kareli-1), resistant (PAUBG-407, PAUBG-200, and PAUBG-1514), moderately resistant (PAUBG-204), highly susceptible (Punjab-14, PAUBG-301, PAUBG-229, and PAUBG-341), susceptible (PAUBG128) and moderately susceptible (PAUBG-394, PAUBG-226, and PAUBG-353) groups. Further, PCR diagnosis indicated the presence of the virus in all samples. The infection
decreased mean chlorophyll (a, b, total), carotenoids, and Fv/Fm ratio more in susceptible genotypes than resistant genotypes over healthy controls. The susceptible genotypes registered higher total carbohydrates and total soluble sugars but the contents of total soluble and crude proteins decreased with increasing DOI. The infection increased phenols, hydrogen peroxide, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), phenyl ammonia-lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) activity more in infected resistant genotypes than in susceptible genotypes. Data was significant (5%) w.r.t genotypes, stages, and treatments. A greater increase in biochemicals in resistant samples may give better protection and the resistant genotypes may aid in crop improvement programs against YMVD.
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Navneet Kaur (2022). Biochemical evaluation of bitter gourd genotypes in response to yellow mosaic virus disease (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.