Assessment of immunity to duck plague virus (duck virus enteritis)
| dc.contributor.advisor | James, P C | |
| dc.contributor.author | Diwakar Dattatrayrao, Kulkarni | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2020-02-26T11:02:22Z | |
| dc.date.available | 2020-02-26T11:02:22Z | |
| dc.date.issued | 1993 | |
| dc.description | PG | en_US |
| dc.description.abstract | During 1991, six outbreak clinically suspected to be duck plague (DP) with 33 per cent morbidity and 26 per cent mortality were investigated Duck plague virus was isolated from each outbreak. The isolates were able to produce the lesions and death of the duck embryos but failed to kill the chicken embryos during initial passages. One of the strains, named DP-S was partially attenuated by 10 passages in chicken .embryos following 20 passages in duck embryos. Though the attenuated strain did kill ducks, its pathogenicity index was reduced from 1.9 to 1,23. The isolate DP-S under transmission electron microscope revealed virions of herpes virus morphology. Two DP vaccines - commercial vaccine and lab-adapted vaccine having virus titres 0.74 and 3.5 log 10 ELD 50/ml respectively, were separately inoculated into four groups of ducklings respectively, two groups receiving single dose and two receiving double dose of corresponding vaccines at an interval of four weeks. Another group of ducklings was kept as control without vaccination. Three ducks in each group were challenged with virulent DPV at four,eight and 20 weeks post-vaccination. The birds in all the five groups were screened at regular intervals for studying the immune response by virus neutralization (VN), leucocyte migration-inhibition (LMI) and passive haemagglutination (PHA) test The challenged and survived birds were screened for the carrier status of DPV by examination of their rectal swabs for virus isolation. In an organized farm, 180 ducks were given commercial vaccine at one year of age and were screened for VN antibodies, LMI response and PHA titres before and eight weeks post -vaccination. Randomly selected two birds were challenged six weeks post-vaccination. The findings of the study are briefly listed as under: Six duck plague outbreaks were investigated, the virus isolated, and characterized. It was partially attenuated in duck and chicken embryos. The commercial, vaccine could elicit very poor immune response as compared to laboratory adapted vaccine. The immunity could not last long even upto eight weeks in single vaccination and 20 weeks in double vaccination. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810143693 | |
| dc.keywords | Synergistic effect of other infections, Virus structure, Carrier state and reservoirs, Prevention and control, Duck plague vaccines in India | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Assessment of immunity | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Assessment of immunity to duck plague virus (duck virus enteritis) | en_US |
| dc.type | Thesis | en_US |