“MOLECULAR DIAGNOSIS AND THERAPEUTIC REGIMEN FOR CAPRINE CONTAGIOUS AGALACTIA”
Loading...
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Nanaji Deshmukh Veterinary Science University, Jabalpur (M.P.)
Abstract
The present work was aimed to study the prevalence, molecular detection and to evolve a suitable therapy against contagious agalactia in goats. For this study, total 705 female lactating goats belonging to organized and unorganized sectors of in and around areas of Jabalpur were screened over a period of 12 months i.e. from April 2014 to march 2015. Out of 705 lactating females, 282 females suspected for contagious agalactia were selected for further study. Seroprevalence of mycoplasmosis was done by slide agglutination test and the presence of Mycoplasma agalactiae was confirmed by polymerase chain reaction (PCR). The DNA required for PCR was extracted from the milk sample. On confirmatory diagnosis of contagious agalactia, 24 clinical cases were randomly grouped as T1, T2, T3 and T4 having 06 goats in each group and were subjected to different therapeutic regimens. Also 06 apparently healthy goats were selected to serve as healthy control (Group C).The response of therapeutic study was evaluated on the basis of clinical score card, clinical parameters and PCR results on day 0 (before treatment) day 7 and day 14 (post treatment).
The overall prevalence of mycoplasmosis in lactating goats using slide agglutination test was 9.50 % while among mastitic cases it was 31.16 %. Overall prevalence of contagious agalactia was 4.39 % and among mastitic goats, it was 14.42%. Mycoplasma agalactiae was detected and confirmed as causative agent of contagious agalactia by using PCR. The prevalence of both mycoplasmosis and CA in organized goatry was significantly higher as compared to unorganized goatry. The age wise prevalence does not differ significantly. Non-descript goats showed lesser prevalence as compared to defined breed of goats under study. The response of therapeutic study revealed significant improvement in temperature, pulse and respiration in all the treatment groups. On the basis of score card and PCR, animal of group T4 showed earliest recovery. Thus, combination of tylosin, tocopherol and sodium selenite was found most efficacious followed by tylosin alone, combination of oxytetracycline, tocopherol and sodium selenite and lastly oxytetracycline alone.