Development of cost effective techniques for in vivo and in vitro propagation of Garlic (Allium sativum L.)
Loading...
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Garlic is one of the most important bulb crops grown and widely used as a spice or condiment throughout India. It is
sexually sterile, hence it is propagated vegetatively. The present investigation was pursued with the view to develop cost
effective techniques for in vivo and in vitro propagation of Garlic (Allium sativum L.). The main objective of the present
investigation was to compare the field performance of plants obtained by in vivo and in vitro techniques. Three different
Treatments were used for raising two garlic cvs. G-282 and HG-17. In Treatment 1, the plantlets regenerated from the vertically
cut cloves as explants, were transferred to the field after hardening. Vertically cut cloves and intact cloves were directly used as
seed in Treatment 2 and 3 respectively Explants as cut above were sterilized by using 0.4 per cent Bevistin (15 min), 70 per cent
Ethanol (30 sec) and 0.1 per cent HgCl2 (10-15 min) before culturing for in vitro propagation. Shoot regeneration response was
observed in both cvs. on MS basal medium supplemented with 3 mg/l 2-iP (shoot regeneration medium). Shoot regeneration
response was 84.4 per cent in cv. G-282, and 85.6 per cent in cv. HG-17. The average number of shoots produced by cvs. G-282
was 3.8 shoots/explant and HG-17 it was3.96 shoots/explant. Rooting response in both cvs. was observed on MS basal medium
supplemented with 1.5 mg/l IAA (rooting medium). Rooting response in regenerated shoots was 80 per cent in cv. G-282, and
77 per cent in cv. HG-17. The average number of roots produced by cvs. G-282 was 12.5 roots/regenerated shoot and HG-17 it
was 11.9 roots/regenerated shoot. In cvs. G-282 and HG-17, 86.0 per cent and 88.4 per cent plants survived during hardening
respectively. The maximum photosynthetic rate was observed in Treatment 3 in both the cvs. G-282 and HG-17 of garlic
followed by Treatment 1 and minimum photosynthetic rate was observed in Treatment 2. A similar trend was observed for
chlorophyll content, nitrogen content, protein content, allicin content and net assimilation rate in both cvs. G-282 and HG-17 of
garlic. The maximum bulb weight was recorded in Treatment 3 in both the cvs. G-282 and HG-17 of garlic followed by
Treatment 1 and minimum bulb weight was observed in Treatment 2. A similar trend was observed for clove weight and number
of cloves/bulb in both cvs. G-282 and HG-17 of garlic. The cv. HG-17 performed better in terms of photosynthetic rate,
chlorophyll content, nitrogen content, protein content, allicin content ,net assimilation rate, clove weight and number of
cloves/bulb as compared to cv. G-282. The G-282 cv. performed better in term of clove weight as compared to cv. HG-17 of
garlic.
Description
Keywords
Multiple shoots, 2-Isopentenyl-adenine (2iP), lAA