Marker assisted selection for fusarium wilt and ascochyta blight resistance in chickpea (Cicer arietinum L.)
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Date
2016
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Publisher
CCSHAU
Abstract
The present investigation on marker assisted selection for resistance to Fusarium wilt and
Ascochyta blight in chickpea was conducted with the objectives to screen the F3
genotypes of two
crosses of chickpea for QTLs conferring resistance to Fusarium wilt and Ascochyta blight and to
phenotype F
3-4 genotypes having QTLs resistant for Fusarium wilt under artificial conditions and
Ascochyta blight under natural conditions. For selection of genotypes carrying QTL(s) for disease
resistance, 96 genotypes of F3 generation along with parents HC 1 and H 91-36, and 68 genotypes of F
3
generation along with their parents GNG 663 and H 04-99 were used for Fusarium wilt and Ascochyta
blight. 20 SSR primers linked to QTLs for resistance to Fusarium wilt (8 SSR primers) and Ascochyta blight
(12 SSR Primers) were used. These 20 SSR were used to discriminate the parental genotypes in which for
wilt 3 SSR primers and for blight 4 SSR primers showed polymorphism in cross HC 1 × H 91-36 while for
wilt 6 SSR primers and for blight 8 SSR primers showed polymorphism in cross GNG 663 × H 04-99. By
using the primers showed polymorphism for both Fusarium wilt and Ascochyta blight, 52 genotypes for
Fusarium wilt and 64 genotypes for Ascochyta blight from cross HC 1 × H 91-36 were found having
resistant QTLs. 50 genotypes for Fusarium wilt and 48 genotypes for Ascochyta blight from cross
GNG 663 × H 04-99 were found having resistant QTLs. In cross HC 1 × H 91-36, 14 genotypes for
Fusarium wilt and 20 genotypes for Ascochyta blight highly resistant while in cross GNG 663 × H 04
99, 20 genotype for Fusarium wilt and 17 genotypes for Ascochyta blight highly resistant were found.
In collective investigation, 3 genotypes highly resistant and 6 genotypes resistant for both Fusarium
wilt and Ascochyta blight were found in the cross HC 1 x H 91-36 and 6 genotypes highly resistant and
2 genotype resistant for both Fusarium wilt Ascochyta blight were found in the cross GNG 663 x H 04-99.
Description
Keywords
Diseases, Fungi, Genotypes, Chickpeas, dna, Crossing over, Planting, Genetics, Biological phenomena, Polymorphism