Characterization of Uromyces viciae-fabae (PERS.) J. SCHRÖT causing lentil rust and molecular tagging for its resistance
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
The population structure of rust isolates infecting lentil and pea in different districts of Punjab was revisited on the basis of cross infectivity test and molecular characterization. Both lentil and pea rust isolates were found non-cross infective at different stages of plant growth. The ITS region of ~700 bp, amplified from DNA of these isolates showed 97 to 99.24% sequence homology with Uromyces viciae-fabae with more than 90% query cover. The sequences were then submitted and accession numbers were provided by GenBank. Further, analysis showed less genetic variation among these isolates. Genetically diverse microsperma and macrosperma lentil lines could not differentiate the rust populations prevalent at Gurdaspur and Ludhiana. Further, recombinant inbred lines (RILs) developed from cross between rust resistant (FLIP-2004-7L) and susceptible (L-9-12) cultivars were phenotyped against lentil rust for genetic analysis and molecular mapping. Genetic analysis indicated the role of major gene in resistance. The phenotypic data was also used to identify molecular markers associated with rust resistance. Out of 389 SSR markers, available in public domain, two markers, namely, LcSSR 440 and LcSSR 606 were found to be linked with rust resistance at 8.3 and 8.1cM, respectively. Availability of high-density linkage maps and genome sequence information in lentil can be helpful to extend the present study in identifying candidate resistance genes against U. viciae-fabae
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