Influence of P. aeruginosa and glutathione on physio-chemical changes in Vigna mungo cultivars under PEG-6000 induced stress
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Date
2019
Authors
KESHARWANI, KOMAL
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Publisher
Department of Biochemistry & Biochemical Engineering Jacob Institute of Biotechnology and Bioengineering Sam Higginbottom University of Agriculture, Technology and Sciences Allahabad
Abstract
In the present study, the physiochemical changes by P. aeruginosa and glutathione under PEG (polyethylene glycol) induced stress were compared in two Vigna mungo varieties. Vigna mungo seeds were subjected to PEG solutions of two different concentration, 7% and 12%, glutathione solution of two different concentration, 50 ml L-1 and 100 ml L-1 and in combination with P. aeruginosa. Various physiological parameters, proline and chlorophyll content and various enzymatic antioxidants invertase, amylase, catalase, superoxide dimutase, glutathione reductase ascorbate peroxidase and non-enzymatic antioxidants GSH (reduced) and ascorbate were measured. Decrease in root length, fresh weight and dry weight was observed under different treatments but P. aeruginosa enhanced the root length, fresh and dry weight. Relative water content, chlorophyll, invertase, amylase, catalase decreased under PEG-6000, but showed enhancement under P. aeruginosa and where as decreased under glutathione. PEG stress reduced ascorbate content while glutathione and P. aeruginosa showed similar effect as control while GSH suddenly increase in both varities. Overall IPU2-43 showed better result than Shekhar-2 in all the parameters studied.
Description
M. Sc.
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