Detection of polymorphism in Kiss1 and GDF9 gene and its association with growth and reproduction traits in indigenous goats
Abstract
The present study was conducted on randomly selected 84Beetal goat, 47 Jakhrana goat and
62 indigenous goat breeds(from farmers flock) to scan the polymorphism in Kiss1 and GDF9 gene and
its association with growth and reproduction traits. Two sets of primers were used to amplify targeted
regions of Kiss1 and GDF9 gene in Beetal, Jakhrana and field goat samples. The PCR product of 242
bp size of targeted region of Kiss1 gene was digested with the restriction enzyme Sac1. All the three
possible genotypes were observed in our resource population. Frequency of C allele of Kiss1 gene was
higher in our studied population goats i.e. 0.89 in Beetal, 0.86 in Jakhrana and 0.73 in field samples.
CC genotype was found to be the predominant genotype Beetal (0.81), Jakhrana (0.72) and goats at
farmers flock (0.51). The 1069 bp PCR product of GDF9 gene was digested with the DdeI enzyme,
resolved into monomorphic pattern AA in field samples but three polymorphic patterns of AA, AB and
BB types in Beetal goat and two genotype in Jakhrana goat. The genotypic and allelic frequencies of
GDF9 gene indicated that the frequency of A allele was highest in Beetal (0.86), Jakhrana (0.98) and
goats at farmers flock (1.00). Effect of Kiss 1 genotype was non-significant in all growth and
reproductive traits except 12M body weight in Beetal goats. CC genotype was having significant higher
(P<0.05) 12M body weight (22.57) than CT genotype (19.64). In field samples, CT genotype had
significant association with litter size. CT and TT genotypes had significantly higher litter size
(P<0.01) than CC genotype indicating that allele T was associated with higher litter size. Effect of GDF
9 genotype was non-significant in all growth and reproductive traits except litter size in Beetal goats.
AB genotyped Beetal goats showed significantly (P<0.05) highest average litter size 1.592±0.07
compared to AA genotype 1.139±0.05 indicating that allele B was associated with higher litter size.
Breed-wise distribution of field samples revealed that Barbari goats showed significantly higher
average litter size (2.857±0.11) compared to Beetal, Non-descript, Sirohi and Sojat goat breeds under
study (P<0.01). The frequency of CT and TT genotype is also comparatively higher in Barbari goats.
In Beetal goat, combined genotypes i.e ABCC (1.67±0.086) and ABCT (1.45±0.141) significantly
revealed highest average litter size compared to other genotypes. (P<0.01).These observations on SNPs
of Kiss1 and GDF9 gene and their association with growth and reproduction traits can be utilized as a
marker after validation of results on large population.
Description
Keywords
null