MOLECULAR CHARACTERIZATION OF ADVANCED BREEDING LINES OF SESAME (Sesamum indicum L.) FOR ALTERNARIA AND PHYLLODY RESISTANCE
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Date
2013-06-30
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UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
Abstract
Two hundred and seventeen F6 families generated from both straight and
reciprocal crosses (RT-273 x GLB and TNL x E8) with fifteen checks were evaluated in
augmented design to find out the extent of genetic variability for yield and different yield
attributing characters and their association with seed yield. The inheritance of Alternaria
and phyllody resistance in F6 families of sesame through RAPD and SSR molecular
markers analysis was also undertaken.
Out of 217 F6 families, 102 F6 families of RT-273 x GLB screened for Alternaria
blight disease showed a segregation ratio of 2.82R:1.17S (fitted to 3:1 ratio) clearly
indicated that the resistance was in the control of a single dominant gene and the 115
families of the TNL X E8 screened for phyllody showed the segregation ratio of
9.73R:6.26S (fitted to 9:7 ratio) revealed that the phyllody resistance was governed by
two dominant genes showing complementary gene action. RAPD OPC13 primer
generated 400 kb band which was associated with susceptibility and absent in resistant
types. RM-9 primer of SSR could amplify four bands instead of one for Alternaria
indicated that it was more like a random primer.
The study revealed a wide range of variability and high heritability for almost all
the traits. The genetic advance, as per cent mean, suggesting still there is a scope for
further improvement of families for these characters. Correlation studies revealed that a
highly similar significant positive magnitude of correlation was recorded by number of
branches per plant, number of capsules per plant, number of seeds per capsule and 1000
seed weight with seed yield. The maximum direct effect on seed yield was exhibited, by
number of branches per plant and distance from ground to first capsule.
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