Preservation of dog semen in three extenders at refrigeration temperature
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Date
1998
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Department of Animal Reproduction, College of Veterinary and animal Sciences, Mannuthy
Abstract
With the ultimate objective of evolving a suitable diluent for preservation of dog semen at 4°C, semen was collected from six mongrel dogs maintained in kennels at Veterinary college hospital, Mannuthy. A total of 36 ejaculates, six from each dog was collected by digital manipulation and physical and morphological characters were evaluated. Three extenders viz., Egg Yolk Tris (TYT), Egg Yolk citrate glycine glucose (EYCGG) and Goat milk (GM) were used for preservation of semen. Sperm motility, percentage of live sperm, abnormal spermatozoa and acrosomal integrity were evaluated at 24 hours interval for five days after preservation at 4°C in the above extenders. Six out of seven dogs showed good response to digital manipulation and ejaculated good quality semen without teaser bitch. The overall mean volume of first, second and third fraction of semen was 0.63 ± 0.07 ml, 1.29 ± 0.08 ml and 4.12 ± 0.23 ml respectively. The colour and consistency of the first and third fraction was clear, watery and second fraction was thickmilkly to thin milkly. The average mass activity of sperm rich fraction of semen was ++(+) and the density was DD. The mean initial sperm motility was 86.67 ± 1.07 per cent.
The mean pH of first, second and third fraction of semen was 6.24 ± 0.01, 6.36 ± 0.01 and 6.65 ± 0.02 respectively. The overall mean spermatozoal concentration of second fraction was 416.28 ± 22.56 million per ml and that third fraction was 6.11 ± 1.66 million per ml. The average total sperm output per ejaculate was 527.50 ± 29.46 million. The overall mean live sperm and abnormal sperm was 89.44 ± 0.57 and 7.59 ± 0.45 per cent. The percentage of acrosomal abnormality was 6.63 ± 0.38. The average time taken for reduction of methylene blue by dog semen was 26.40 ± 0.86 minutes. The mean percentage of sperm motility at 0,10,20 and 30 minutes of incubation (46.5°C) was 86.38 ± 1.04, 88.33 ± 1.13, 70.55 ± 1.26 and 53.2 ± 2.17 respectively. There was significant (Pminutes of incubation and sperm motility upto 5 days of preservation under refrigeration temperature. The percentage of sperm motility upto day 5 was significantly higher in Egg Yolk Tris (49.86 per cent) and Egg Yolk citrate Glycine Glucose (48.33 per cent) than in Goat milk (0 per cent).
There was significantly higher percentage of live sperms and lower percentage of abnormal sperms and acrosomal damage in EYT and EYCGG than in GM. Eventhough the values are not statistically significant among EYT and EYCGG, EYT was found to have higher percentage of sperm motility and live sperm, lower percentage of abnormal sperms and acrosomal damage when compared to EYCGG. Besides EYT was also found to have better clarity for microscopical examination. Hence it could be inferred that Egg yolk tris is superior to Egg yolk citrate glycine glucose and goat milk for preservation of dog semen at 4°C.
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