STUDIES ON EFFECT OF PROBIOTIC COMBINATION (BACILLUS SUBTILIS + BACILLUS COAGULANS) AGAINST ENTEROPATHOGENIC ESCHERICHIA COLI-INDUCED DIARRHOEA IN DOGS
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Date
2019-04-26
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pvnrtvu
Abstract
A total of 24 community dogs (14 males and 10 females) with average age
of 2 years were procured and acclimatized for 1 week. The dogs were randomly
divided into 4 groups consisting of 6 dogs in each and kept in kennel room and
maintained in the experimental canine facility, Veterinary Clinical Complex (VCC),
Rajendranagar, Hyderabad. Treatment was scheduled for a period of 10 days.
Group 1 was kept as Entero Pathogenic Escherichia coli (EPEC) control and given
2.4 x 109 bacteria/ml per oral route in the form of milk; group 2 served as EPEC
(2.4x109 bacteria/ml)+ Probiotic combination @ 2 billions/cfu/gram, per oral route in
the form of milk/water, twice a day; group 3 was kept as EPEC (2.4x109
bacteria/ml) + Amikacin @15 mg/kg body weight by intramuscular route and group
4 served as EPEC (2.4 x 109 bacteria/ml) + Probiotic combination @ (2
billions/cfu/gram), per oral route in the form of milk/water, twice a day + Amikacin
@15 mg/kg body weight by intramuscular route. Blood samples were collected on
day 1, 5 and day 10 for haematology and sera samples were separated for the
estimation of biochemical and antioxidant parameters. Faecal samples were also
collected for estimation of short chain fatty acids and bacterial load in the gut after
the treatment schedule.
The present study revealed significant alterations in total protein, albumin,
HDL cholesterol, blood urea Nitrogen, creatinine, glucose, sodium , potassium,
chloride, haemotology (RBC, WBC, Hb and PCV) and antioxidant profile
(TBARS, GSH, SOD and catalase) in EPEC control group 1 as compared to
treatment groups 2, 3 and 4. The short chain fatty acids and total bacterial load
in gut were also increased significantly (p˂0.05) in groups 2, 3 and 4 after 10
day treatment schedule.
The results of the investigation revealed that probiotics seem to be safe and
have clear beneficial effects in shortening the duration and reducing stool
frequency in acute infectious diarrhoea by competitive exclusion and production
of lactosporin, which inhibits the growth of pathogenic E. coli and other
pathogenic microbiome in the gut and also stabilizing antioxidant defences.
Description
D-634
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