Age Correlated Histomorphological And Histochemical Studies On The Mammary Gland Of Indian Buffalo (Bubalus Bubalis)
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Date
2000
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
Histomorphochemical studies on pre- and post -natal development of mammary
gland revealed that mammary bud was first observed at 90-109 days of foetal age which
was comprised of centrally located primary sprout embedded in mesenchymal tissue.
This mesenchymal tissue differentiated into connective tissue whorls. The primary sprout
became canalized and gave rise to secondary sprouts at 120-146 days of foetal age. The
mammary anlage at 152-182 days of foetal age showed canalized primary and secondary
sprouts differentiated into streak canal, teat sinus and primitive gland sinus. The teat was
fully dif ferentiated into streak canal, Furstenberg's rosette, teat sinus by 251-254 days of
foetal life. The gland sinus was dif ferentiated into duct system of mammary gland. At
birth, mammary gland of buffalo consisted of small teat having streak canal,
Furstenberg's rosette, gland sinus and extensive duct system lined by stratified cuboidal
to stratified columnar epithelium. In prepubertal buffalo, mammary gland showed
extensive duct system with clump of glandular tissue at the terminal ends of ducts. In
adults, the percentage of glandular tissue was significantly higher in lactating buf faloes
than non -lactating buffaloes. The alveoli were highly developed and vary greately in
shape and size in lactating buffalo. These alveoli get shrunkened in non -lactating
buffaloes. Histochemical observations revealed that NMPS and basic proteins were better
demonstrated in lactating mammary gland, but AMPS were more in non -lactating animals.
Histoenzymic study revealed that epithelium of teat, alveoli and ducts were positive for
AKPase, ACPase, G-6-Pase, NSE, G -6 -PD, SDH, LDH, GLD, NADPH-diaphorase,
NADH-diaphorase whereas stroma was positive for ATPase, SDH, NADPH-diaphorase,
NADH-diaphorase and G -6 -PD. ACh Ease was only seen in neuronal elements.