Allele mining for drought related antioxidant genes in Cucumis spp.
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Date
2012
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Department of Biotechnology COLLEGE OF AGRICULTURE, BIKANER SWAMI KESHWANAND RAJASTHAN AGRICULTURAL UNIVERSITY BIKANER-334006 (RAJ.)
Abstract
A study was conducted to evaluate 44 genotypes of Ten Cucumis spp. with respect to drought tolerance and to determine its effect on various morpho-physiological (root length, shoot length, root and shoot fresh and dry weight) and biochemical (Catalase, Ascorbate peroxidase and Guiaicol peroxidase) parameters. Of these 44 genotypes 11.36% were tolerant, 29.54% were moderately tolerant and 38.63% were moderately susceptible and 20.45% were susceptible. Decrease in root and shoot length, root and shoot fresh weight, root and shoot dry weight was observed in most of the genotypes. The activity of Catalase and GPOX was increased in water deficit conditions while the activity of APOX decreased most of the genotypes. Though none of the growth parameters and enzyme activity was correlated with tolerance level, various morpho-physiological and biochemical parameters, were associated under control and stress conditions. Allelic variations in the form of SNPs were observed between and within various Cucumis spp. studied. Transition, transversion and indels were obtained at different positions in the sequences. Variation between genotypes were observed at various sequence positions while in between the species variations was observed for C.melo and C.sativus and C.callosus. These variation could be important for the dissimilarity of catalase enzyme activity in different genotypes of Cucumis spp. under water stress condition.
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