MOLECULAR MARKER PROFILING AND ITS ASSOCIATION WITH ECONOMIC TRAITS OF POPULAR INDIAN CULTIVARS OF POMEGRANATE (Punica granatum L.)
Loading...
Date
2017-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT
Abstract
The present investigation was carried out with 25 pomegranate genotypes at
University of Horticultural Sciences, Bagalkot, Karnataka during 2016-17 using
randomized complete block design with three replications. These genotypes included
popular cultivars, four mutant lines and two wild forms, which were subjected to
extensive phenotypic characterization using morphological and biochemical parameters
together with molecular marker profiling using 24 microsatellite markers as well as
field screening for resistance to bacterial blight and foliar and fruit spots.
Variability studies demonstrated higher PCV, GCV, heritability and GAM
implying the existence of sufficient variation among the studied genotypes. High
heritability with high genetic advance was recorded for most of the characters viz. fruit
weight, fruit volume, peel weight, aril weight, total number of arils and titratable acidity
indicating effective selection for these traits
Many of the cultivars studied, exhibited desirable phenotypes like soft seediness,
red juice and round shape. Daru and Nana were found to have very less bacterial blight
severity as compared to the popular cultivars like Bhagwa, Early Bhagwa and Ganesh.
In the principle component analysis, 35 characters analysed were partitioned in to three
PCs with the cumulative variance of 55.37%. Correlation studies revealed significant
positive correlation between fruit weight and fruit volume, fresh and dry weight of 100
arils, peel weight, aril weight and total number of arils per fruit while path coefficient
analyses affirmed a significant contribution of peel and aril weight on fruit weight.
D2analysis grouped 23 genotypes into four distinct clusters and high genetic
divergence was found for genotypes falling in clusters II and III. Based on marker trait
association, markers like PGKVR112, PGKVR 132 and PGKVR 149 exhibited
significant R2 value for aril parameters, fruit weight and fruit volume while, marker
PGKVR 122 was found to be associated with bacterial blight and also with fruit weight.
Consequently, the present investigation illustrated existence of wide range of
variations for most of the characters among the pomegranate genotypes, providing
opportunities for genetic gain through selection or hybridization in future breeding
programme.
Description
Keywords
null