Isolation and Characterization of Diazotrophic and Phosphate Solubilizing Bacteria in Land Use Systems of Doda District, J&K
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Date
2018-10
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Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jamu
Abstract
A soil survey was conducted at Doda district in the month of April 2016 to assess the various physico-chemical and biological properties of the soil. Composite surface soil samples (185) were collected at a depth of 0-15cm. For microbial analysis rhizosphere fresh soil samples were collected from different land use systems and stored at 4°C in a freezer. The exact sample location was recorded using a handheld GPS receiver. Geographic Information System (GIS) and Inverse distance weighting (IDW) technique was adopted to generate prediction maps of various soil properties
Biochemical tests (catalase, nitrate reduction, citrate utilization and oxidase test) performed on bacterial isolates of diazotrophic and P-solubising bacteria in different land use systems of doda district showed positive results for majority of samples and a few showed negative results as well. The maximum soil pH and EC was recorded higher under barren lands with maximum values being 7.90 and 0.77 dS m-1. The maximum OC was recorded under forest 1.19 percent. The highest Sand percentage was 74.90 percent under barren land.
The highest Silt percentage was 46.20 percent under agriculture. The maximum Clay percentage was 61.10 percent under forest. The highest available nitrogen was 551.29 kg ha-1 under forest. The available phosphorus recorded was higher in agriculture land use systems 27.51 kg ha-1. The available potassium was recorded higher under forests 454.23 kg ha-1. The available sulphur was recorded higher in forests 21.55 kg ha-1. The exchangeable calcium, magnesium and sodium contents were recorded higher in barren lands with maximum values being 6.18, 1.75 m eq 100g-1 and 97.56 mg kg-1.
The available zinc, iron, copper and manganese were recorded higher in forest land use systems with maximum values being 3.12, 27.36, 3.38 and 24.28 mg kg-1 respectively. Thus the present study revealed that presence of both gram positive and gram negative bacteria in land use systems of Doda district suggest the wide diversity of diazotrophes and P-solubilizers. Soil fertility maps along with a ready database of soil information at district level will benefit the farmers in determining site specific nutrient management to maintain soil health.
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