Age related histomorphochemical studies on Ovaries of Punjab white quail
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Date
2015
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The present study was undertaken to elucidate gross, histomorphological, histochemical studies on ovaries of Punjab white quail. The birds were divided into four age groups 8 weeks, 16 weeks, 24 weeks and 30 weeks. The gross morphological studies revealed that only left ovary was present in all age groups of Punjab white quail. The shape of ovary was ovoid at 8 weeks of age, became irregular at 16 weeks and resembled bunch of grapes at 24 weeks and 30 weeks of age. Ovarian weight, length and volume increased with age but were maximum at 24 weeks. Histologically, cortex and medulla was well differentiated at 8 weeks and 16 weeks of age whereas at 24 and 30 weeks, the demarcation was lost. No follicle was found with 2 or 3 oocytes or oocyte with 2 nuclei. Hence the chances of double yolked eggs are rare. Primordial follicles were present in groups and were maximum at 8 weeks of age. Crescent shaped balbiani body was present in primary follicles which became spherical in secondary follicles. Corona radiata was reported first time in secondary follicles. All the layers were present in tertiary follicles viz. vitelline membrane, corona radiata, granulosa layer, theca interna, theca externa, superficial tunic and surface epithelium. Oocyte became vitellogenic as filled with yolk granules. Medulla contained abundance of collagen fibers along with few reticular and elastic fibers and lacunar channels increased with the advancing age of birds. The distribution of histochemical moieties did not vary in different age groups but the reactions were variable in various structures of ovary. The high amount of acid mucoploysaccharides in atretic follicles suggests atresia. Micrometrical data showed the height of epithelium and diameter of follicles was maximum at 24 weeks. The thickness of cortex and medulla was maximum at 8weeks and 16 weeks. It is inferred from the present study that reproductive efficiency of birds at 24 weeks of age is maximum, as all the structures were more pronounced at 24 weeks of age in ovaries of Punjab white quail