STUDIES ON IN VITRO MUTAGENESIS IN BANANA
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Date
2013-09
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K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Abstract
Studies on in vitro mutagenesis in Banana was carried out in the Department
of Crop Improvement and Biotechnology, KRCCH, Arabhavi during 2012-2013.
Three varieties of banana were used for the experiment Rajapuri bale (AAB), Yelakki
bale (AAB) and Nanjangudu rasbale (AAB). Callus & multiple bud cultures were
irradiated with gamma rays using 60Co at BARC, Mumbai. The young male
inflorescence was used for callus induction with 2 mg/l of 2, 4-D, among the varieties,
Rajapuri bale recorded over all better callusing followed by Nanjangudu rasbale and
Yellaki bale. Callai of different varieties did not show any regeneration after gamma
irradiation. However, unirradiated calli produced embryo like structures after long
period of incubation of six months.
Multiple bud culture raised by shoot tip culture was also irradiated. Among the
different gamma irradiation maximum survival and regeneration was recorded in
25 Gy (53.33% and 45.00%) as compared to control (94.66% and 91.66%)
respectively. There was reduction in number of shoots produced, number of leaves per
shoot and shoot length with increased doses of irradiation. Growth was better in lower
doses of gamma irradiation as compared to higher doses. The inhibitory effect of
gamma irradiation on in vitro root growth was noticed. Maximum rooting per cent
and root growth was noticed in unirradiated control, whereas decline in growth was
observed with increased dose of gamma irradiation.
The putative mutants were observed after hardening, there were Dwarf
mutants at 65 and 55 Gy, mini dwarf mutant at 65 Gy, chlorophyll mutant at 35, 45,
55 and 65 Gy while pigmented mutants were noticed only at 25 Gy. These mutants
can be field evaluated to confirm their stability and performance.
Description
TH474
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