MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF BIRD OF PARADISE (Strelitzia reginae L.) GENOTYPES
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Date
2016-11
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K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Abstract
The study undertaken on the genetic variability in Bird of Paradise genotypes at the Department of Biotechnology and Crop Improvement, Kittur Rani Chennamma College of Horticulture, Arabhavi. Forty cultivars used for the studies were collected from Horticultural Research Station Kanbargi Farm, Belgaum. The experimental data was subjected to statistical analysis for elucidating the information on genetic variation existing for different components of growth parameters, and flowering parameters.
The genetic variation was assessed using parameters like genotypic (GV) and Phenotypic variances (PV), genotypic (GCV) and phenotypic (PCV) coefficient of variation, heritability (broad sense) and genetic advance as per cent over mean (GAM). The inter-character correlation and path coefficient analysis were also carried out to know the relationship among various growth and yield components. The genetic divergence were computed by D2 analysis, genetic closeness and divergence were assessed. The highest inter cluster distance was observed between cluster III and IX and the lowest between cluster VIII and X. The highest intracluster distance was observed in cluster II and lowest in cluster VII. So, by intercrossing the divergent genotypes, i.e., with maximum intercluster distance, we can utilize hidden variability and produce good recombinations in advancing generations.
Inter clustering of divergent genotypes would lead to greater opportunity for crossing over, which releases hidden variability by breaking linkage. Progeny derived from such diverse crosses are expected to show wide spectrum of variability provided a greater scope for isolating transgressive segregants in the advance generation.
FRAPD profiles for 10 genotypes were generated with 3 random 20 mer primers. Ten Bird of Paradise genotypes were grouped into two major clusters using a Jaccard’s similarity coefficient of 0.20. 1st cluster is containing 8 genotypes (G8, G5, G2, G4, G1, G3, G6, and G7). Cluster second is containing 2 genotypes G9 and G10. The present study also shows that FRAPD markers can be useful tool for the assessment of genetic variability among Bird of Paradise genotypes.
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TH584
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