Study of genetic diversity of Fusarium species causing wilt in safflower by using its marker
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Date
2011-08-17
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Safflower wilt is a major disease caused by the genus Fusarium, is a widely
distributed phytopathogen and brings about great economic loss of crops. Fusarium
species presents a high biological genetic variability. With the goal of evaluating the
genetic relationship among different Fusarium species observed in crops, an
experiment was carried out involving the isolation of fungal pathogen from eight
different safflower growing areas in Marathwada regions of Maharashtra state.
Genetic variability was detected by comparison of the isolates at molecular level by
comparing the nucleotide sequences of ITS regions using universal fungal primer pair
(1TS-1 and ITS-4). Three Fusarium species were obtained from eight Fusarium
isolates viz., Fusarium equiseti, Fusarium moniliforme and Fusarium oxysporum and
all were found to be pathogenic to safflower. Phylogenetic studies of all isolates
revealed the inter-relationship among them. The phylogenetic tree based on ITS
sequence data divided all the eight isolates into two groups having same origin.
Fusarium oxysporum and Fusarium equiseti obtained in same group and showed
more similarity in comparison to Fusarium moniliforme. Divergence among particular
species showed the occurrence of their strain or races.
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