Morphological and molecular characterization in brinjal (Solanum melongena L.)
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
Morphological and molecular characterization in brinjal (Solanum melongena L.) was carried with 110 germplasm lines on the basis of 33 morphological descriptors and 40 Simple Sequence Repeat (SSR) markers. Highest estimates of PCV (51.66%) and GCV (50.68%) were observed for number of fruits per plant. High heritability and high genetic advance for average fruit weight, number of fruits per plant, fruit yield per plant, fruit length, fruit breadth, and plant height revealed preponderance of additive gene action. Fruit yield per plant showed highly significant and positive correlation with number of fruits per plant (0.495) followed by leaf blade length (0.465), leaf blade width (0.347) at genotypic level. First five PC’s shared 71.90% of the total variation that was mainly contributed by leaf dimensions, fruit pedicel length, fruit length and breadth, fruit yield per plant and average fruit weight. Mahalanobis D2statistic grouped 110 brinjal lines into eleven broad clusters with the maximum genotypes in cluster IV, but single genotypes in each of clusters II, V, IX, X and XI. The highest intra-cluster D2 (74.43) and inter-cluster D2 (1584.40) were unveiled in cluster VII and between cluster VIII and X. The highest mean values for average fruit weight and number of fruits per plant were displayed in Cluster VIII and X, respectively. The molecular investigation with 19 SSR primers pairs (out of 40) amplified 98 effective alleles with an average of 4.39 effective alleles per marker. The highest value for number of effective alleles (9.09), Shannon’s diversity index (2.29), expected heterozygosity (0.89) and polymorphism information content (0.88) was revealed for locus EEMS28.UPGMA based Jaccard’s similarity coefficient of molecular data estimated genetic dissimilarity (0.35- 1.00) with an average dissimilarity of 0.675 across the genotypes and divided the same into major three groups and two outlines (PBR-101 and PBR-139).
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