ASSESSMENT OF RECOMBINANT INBRED LINES (RILs) FOR BIOCHEMICAL CHARACTERISTICS ASSOCIATED WITH ABIOTIC AND BIOTIC STRESSES IN Brassica juncea (L.) Czern. and Coss.
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Date
2011
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PAU
Abstract
The present study was aimed at assessing the recombinant inbred lines (RILs) of Brassica juncea for biochemical parameters viz peroxidase (POD), phenylalanine ammonia lyase (PAL), total phenols, O-hydroxy phenols, proline, carotenoids, glutathione (GSH), tocopherols and glucosinolates, associated with resistance against fungal diseases; alternaria blight, white rust and high temperature stress. RILs were analysed phenotypically for alternaria blight and white rust severity under normal field conditions. Alternaria blight severity ranged from 30.56 to 83.33% whereas white rust severity ranged from 0 to 22.3%. None of the lines was resistant against alternaria blight whereas 46 lines showed 0% white rust severity. High temperature stress evalution was done on seven day old seedlings at LT50 conditions (45˚C, 6 hrs, 70% humidity). The survival percentage in RILs ranged from 0 to 100% with a mean value of 64.77. 30 lines had 100% survival. The values for POD, PAL, total phenols, O-hydroxy phenols, proline, carotenoids, glutathione, tocopherols and glucosinolates ranged from 0.05-8.46 (ΔE/min/g FW tissue), 0.214-1.754 (nmole/g FW tissue), 3.46-13.12 (mg/g DW tissue), 0.212-6.30 (mg/g DW tissue), 10.67-46.06 (μmole/g DW tissue), 19.01-92.54 (nmole/g DW tissue), 0.038-0.310 (nmole/g FW tissue), 32.2-1629.52 (ppm) and 21.27-108.50 (μmole/g FW tissue) respectively. Correlation studies depicted significant negative correlation of PAL (-0.184*) and O-hydroxy phenols (-0.196*) with alternaria blight. GSH showed a significant negative correlation with alternaria blight (-0.228**) and white rust (-0.179*). A significant positive correlation (0.160*) was observed between proline and survival percentage under high temperature stress. The above results suggest the protective role of PAL and O-hydroxy phenols against alternaria blight and GSH against both the diseases and proline against high temperature stress. From the present study, nine elite lines having desirable biochemical parameter for resistant to biotic and abiotic stresses were selected. These lines can be used by breeders in their breeding programme for resistance against alternaria blight, white rust and high temperature stress.
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