Biochemical basis of salt tolerance in wheat landrace derivatives and Gly II transformed lines
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Date
2017
Authors
Lovenpreet Kaur
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Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present investigation was aimed to study the effect of salt stress (150 mM and 250 mM
NaCl) on antioxidant defence system in Gly II transformed lines alongwith check PBW 621
and Kharchia lines raised hydroponically. Various biochemical and morpho-physiological
parameters were recorded in salt tolerant (Kharchia 65, Kharchia local), Gly II transformed
lines (G-2-2, G-3-4, G-1-13) and PBW 621 (non transgenic) genotypes of wheat grown under
laboratory conditions. Growth parameters recorded at 15 days after germination revealed
better performance of Kharchia 65 and G-1-13 as compared to other genotypes. Salinity stress
enhanced activities of superoxide dismutase, glyoxalase II (Gly II), glutathione reductase
(GR), guaiacol peroxidase (GPX), glutathione s transferase (GST) in all genotypes.
Apparently, increase in Na+ and decrease in K+ was recorded in leaves of all the genotypes
except in Kharchia 65 where Na+ accumulation was lesser. The contents of glutathione
increased in all genotypes while ascorbic acid was highest in G-1-13 and Kharchia 65
genotypes. Soluble sugar content increased maximum in G-3-4 and Kharchia 65. The higher
concentration of H2O2 and electrolyte leakage was recorded in PBW 621 (check). Gly II
transformed lines exhibited superior salt tolerance over PBW 621 by accumulating higher
content of glutathione with increasing levels of GR, GPX and Gly II activities. The
upregulation of antioxidant defence system and less accumulation of Na+ ions, H2O2 and
TBARS content in gly II transformed lines and Kharchia lines might have played role in
salinity tolerance.
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