BIOMETRICAL AND MOLECULAR DISSECTION OF GENETIC DIVERSITY IN MINICORE COLLECTION OF PIGEONPEA (Cajanus cajan (L.) Millsp.) FOR Fusarium WILT, SMD AND YIELD
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Date
2014-06-30
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Abstract
The present investigation was carried out using 191 minicore collections of pigeonpea
with 5 check varieties to know the genetic diversity, to ascertain the reaction of genotypes to
Fusarium wilt (FW) and Sterility Mosaic Disease (SMD), validation of DNA markers linked
to wilt and SMD, morphological characterisation and identification of stable genotypes for
environments.
High phenotypic co-efficient of variability (PCV), genotypic co-efficient of variability
(GCV), heritability and per cent genetic advance was observed for number of branches per
plant, pod bearing length, number of pods per plant and seed yield per plant. High rate (0.30
to 0.99) of positive direct effect on yield was observed by means of days to 50 % flowering,
number of pods per plant and 100 seed weight at genotypic and phenotypic levels.
Grouping 196 genotypes showed positively skewed distribution with 13 clusters. Cluster I
was the largest comprising 80 genotypes followed by cluster III (51 genotypes). Six clusters
had solitary entries (IPPFC-43, GRG-281-1, ICP-3576, ICP-14116, ICP-13673 and ICPB
2043).
Two years of field screening for Fusarium wilt yielded eleven resistant genotypes of
which, ICP-8949, GRG-2009, ICP-11320, GRG-811 and GRG-333 were wilt resistant and
high yielding. A total of 69 genotypes showed moderate resistant to wilt. The genotypes viz.,
Bahar, ICP-7035, ICP-11910 and RAJA were found resistant for SMD.
Molecular diversity using 18 polymorphic Simple Sequence Repeat (SSR) markers
divided genotypes into 15 clusters, of which ICP-11059 and AK-101 were solitary.
Validation of two Sequence Characterised Amplified Region (SCAR) markers viz., SCAR-
704 (Fusarium wilt) and SCAR-N-18, (SMD) revealed significant association of SCAR-N-18
with SMD.
Stability parameters for seed yield per plant indicated that ASHA(ch) was stable and
desirable, followed by RVK-275 and GRG-811. Morphological characterisation
differentiated ICP-7148 and GC-11-39 from rest of genotypes for determinate growth.
Similarly, ICP-7148 and Gulyal (white) can be distinguished by oblong leaf shape.
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