A STUDY ON BIOCHEMICAL AND HORMONAL PROFILES OF FOLLICULAR FLUID DURING PEAK BREEDING SEASON OF BUFFALOES (Bubalus bubalis)
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Date
2012
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Anand Agricultural University, Anand
Abstract
The present study was planned to estimate biochemical and hormonal profiles of
follicular fluid during peak breeding season of buffaloes (Bubalus bubalis). Two hundred and
ten ovaries from the sexually matured buffaloes were collected from local abattoir during
peak breeding season. The number of ovaries collected with and without corpus luteum was
119 and 91, respectively. The follicles of ovaries were classified in to three categories on the
basis of its diameter: (a) small (1 to 4 mm), (b) medium (5 to 8 mm) and (c) large (9 to
12 mm). Further, the ovaries were classified as per the presence or absence of corpus luteum
(with and without CL). The follicle size was measured with digital Vernier Caliper. Follicular
fluid was aspirated from all three categories of follicles of ovaries with and without corpus
luteum by using a 22-gauge needle attached to a 1 ml plastic syringe and was pooled on a
weekly basis. The total follicular fluid volume of small, medium and large follicles of ovaries
with CL was 18.00, 18.00 and 33.00 ml and of without CL was 9.00, 6.00 and 9.00 ml,
respectively. The follicular fluid was made cell-free by centrifuging at 5°C at 1500 r.p.m. for
30 min. The aliquots of follicular fluid were kept in a 5 ml eppendorfs tubes and stored in a
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Keywords
Veterinary Biochemistry, Study, Study