EFFECT OF DIFFERENT EXPLANTS AND TISSUE CULTURE MEDIA ON CAPSAICIN SYNTHESIS IN CAPSICUM
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Date
2013
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Punjab Agricultural University, Ludhiana
Abstract
Capsaicin biosynthesis was studied in callus cultures derived from root tip and shoot
tip of Capsicum annuum varieties viz. Bharath and Indra. In callus cultures of two varieties,
the activity of all the four enzymes, viz. PAL,TAL, CA4H and CS increased till 15 or 12 days
when induced on media supplemented with 2mgl-1 2,4-D and 0.5mgl-1 kin, but after increasing
the concentration of 2,4-D, there was an early decrease in activity of enzymes. Capsaicin
content in root-tip derived callus cultures of both varieties was much higher than that in
shoot-tip derived callus cultures. Capsaicin content was maximum in root-tip derived callus
cultures of Bharath (90 mgg-1 DW) and minimum in Indra (79 mgg-1 DW). When the callus
cultures were induced on media which was supplemented with NAA, the activity of all the
enzymes and capsaicin content was maximum on media supplemented with 2mgl-1 2, 4-D and
2mgl-1 NAA and 0.5mgl-1 kin, the activity of enzymes was lowered with increase in
concentration of NAA. Sucrose and phosphorus depleted media showed a marginal increase
in capsaicin content and activity of enzymes in both the root tip and shoot-tip derived callus
cultures of both varieties. Nitrogen depleted (ammonium nitrate and potassium nitrate)
medium showed about 7 fold increase in capsaicin content and upto 15,9 and 3 and 2 fold
increase was observed in activity of CA4H, CS, TAL and PAL enzymes respectively
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Keywords
sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas