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Kerala Agricultural University, Thrissur

The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively. Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively. On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs. In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc. During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU). Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.

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2010 - 2014432
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End date: 2014 × Start date: 2010 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Cryopreservation of chethikoduveli (Plumbago rosea L.) and assessment of genetic fidelity of regenerated plantlets using molecular markers
    (Department of Plant Biotechnology, College of Agriculture, Vellayani, 2014) Anand, Vishnu Prakash; KAU
    Investigations on “Cryopreservation of Chethikoduveli (Plumbago rosea L.) and assessment of genetic fidelity of regenerated plantlets using molecular markers” were carried out at the Department of Plant Biotechnology, College of Agriculture, Vellayani during 2011-2013. Plumbago rosea var. Agni plants were collected from AMPRS, Odakkali, Ernakulam and maintained at the Department of Plant Biotechnology, College of Agriculture, Vellayani as source of explant during the course of the study. The objectives of the present study was to standardise cryopreservation protocol by encapsulation dehydration technique for long term conservation of P. rosea and genetic fidelity assessment of plantlets recovered and regenerated from cryostorage using molecular markers. The project was carried out in two phases viz., in vitro regeneration and in vitro conservation by cryopreservation of P. rosea. In vitro regeneration protocol was optimised for P. rosea var. Agni. Various steps of in vitro regeneration viz., surface sterilization, axillary shoot proliferation, in vitro rooting and acclimatization and planting out has been standardised. For surface sterilizing, single nodal explants (3-4 cm long) were subjected to fungicide treatment with 0.1 per cent carbendazim 50 per cent W. P. (for 30 min) followed by aseptic sterilisation dip with absolute alcohol. Further, the explants were surface sterilised with 0.2 per cent mercuric chloride (for 5 min) which gave 100 per cent survival without any contamination. Enhanced release of axillary buds from single nodal explants, with maximum shoot proliferation (5.28 shoots/culture) was obtained in the medium, MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1. The best response (10.67 roots/culture) of in vitro rooting of plantlets was obtained in the medium, MS + NAA 1.0 mg l-1. In vitro rooted plants gave a maximum survival rate of 76 per cent and 72 per cent, when planted out in potting media consisting of red soil and coir pith (3:1) and red soil and coir pith (2:1) supplemented with VAM respectively at 25 per cent shade. In cryopreservation studies, preconditioning treatment (sucrose 0.5 M for 7 days) recorded maximum shoot proliferation (2.67 shoots/culture) when nodal segments with single axillary bud were cultured on MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1 medium. Among different encapsulation treatments, maximum shoot proliferation of (2.31 shoots/culture) was obtained in beads formed with sodium alginate 2.5 per cent and calcium chloride 100 mM, when cultured on the medium, MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1. Pre-culture medium supplemented with sucrose 0.5 M for 3days gave maximum shoot proliferation (3.44 shoots/culture) when cultured on the medium, MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1. A desiccation duration of 5 h at 18.13 per cent moisture level was found to be most effective giving 66.67 per cent survival and 62.50 per cent regeneration on thawing and culturing on the recovery medium MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1. The beads when stored in liquid nitrogen for different duration and cultured on recovery medium did not show any significant variation with respect to survival per cent. RAPD markers were tried to study the genetic fidelity of the regenerated plantlets from encapsulated and cryopreserved axillary buds. Six primers were screened and RAPD banding patterns of the cryoregenerated plantlets and control plants were compared. Polymorphism was not found with any of the primers tested. RAPD profiles of cryoregenerated plantlets were identical to those of the control. The in vitro regeneration protocol optimized included surface sterilization of single node cuttings with 0.2 per cent HgCl2 for 5 min, axillary shoot proliferation in MS medium supplemented with BA 1.5 mg l-1 and IAA 1.0 mg l-1, in vitro rooting in MS medium supplemented with NAA 1.0 mg l-1 and planting out in potting medium, red soil and coir pith (3:1). The protocol for encapsulation dehydration technique of cryopreservation was standardised for the axillary buds of P. rosea with preconditioning in semi solid MS medium supplemented with sucrose 0.5 M for 7 days, encapsulation using sodium alginate 2.5 per cent and calcium chloride 100 mM followed by pre-culture in liquid MS supplemented with sucrose 0.5 M for 3 days and 5 h dehydration (MC 18.13 %), rapid freezing in LN for at least 2 h and recovery in the medium MS + BA 1.5 mg l-1 + IAA 1.0 mg l-1. The cryopreservation protocol using encapsulation-dehydration technique standardised could be utilised for long-term conservation of P. rosea.
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    ThesisItemOpen Access
    Invitro propagation in ashoka : saraca asoca (Roxb.) de wilde.
    (Department of Plant Breeding and Genetics, College of Horticulture, Vellanikkara, 2011) Brindha Devi, I; KAU; Sreenivasan, E
    Asoka (Saraca asoca) is an important medicinal and ornamental tropical tree currently facing the threat of extinction due to overexploitation of trees occurring in forests and other natural habitats. Unscientific and destructive extraction of bark from trees has lead to acute shortage of raw bark by ayurvedic industries. Hence, the International Union for Conservation of Nature and Natural Resources (IUCN) has listed this species under ‘globally vulnerable’ category. It is also enlisted among the 36 threatened and endangered medicinal plants of India. It is considered as the sacred tree of buddhists and Hindus. Literally the term ‘asoca’ means ‘sorrow-less’ and the tree is believed to remove the grief and unhappiness. The tree has immense medicinal properties. Its bark is considered as the primary medicinal part. Due to its acute short supply compared to its demand, various development and research activities are being prioritized to conserve, utilize and improve this species. It is mainly propagated by seeds. Due to heterozygous and cross pollinated nature of the species, it never gives a true to type progeny. Therefore the present study was undertaken to standardize the technique of in vitro propagation of Saraca asoca. Standardization of suitable explants, surface sterilization procedures and culture establishment protocol, Induction of multiple shoots and Elongation of root, hardening and planting out are the major objectives of the study. Nodal segment, Internodal segment and shoot tip were the three explants tried. Various surface sterilization procedures were tried using Chloramphenicol, ethyl alcohol, 0.1% mercuric chloride and combination of ethyl alcohol and mercuric chloride in various concentration and duration, using nodal segments as explant. Surface sterilization using 70% ethyl alcohol for 3 minutes followed by 0.1% mercuric chloride for minutes proved to be the best, which gave the maximum survival percentage of 80.   The next part of the study was standardisation of suitable explants for culture establishment. Among the three explants, Nodal segments gave maximum response of 60 per cent in ½ MS medium with BAP 0.5mg/l. This was followed by shoot tips in the same medium, which gave 10 per cent response. Internodal segments did not respond in any of the media used. Standardisation of basal media for culture establishment was done using nodal segments as the explants. Three media supplemented with BA 0.5 mg.l-1 were tried viz. MS, Half strenght MS, Woody plant media. Among the three, ½ MS media was identified as the best basal medium followed by MS medium. No response was seen in WPM medium. Culture establishment as well as Shoot bud initiation was attempted in ½ MS and MS media with various growth regulator combinations. Maximum response of 60 per cent was obtained in ½ MS medium containing BAP 0.5 mg/l followed by 30 percent in the same medium containing BAP 1.5 mg/l. There were no response with 2,4-D. The response obtained was callusing in all cases. Induction of multiple shooting was tried in ½ MS medium supplemented with BAP, and Kn alone as well as combinations of BAP, IAA at various concentration. Here highest response of 30 per cent of single shoots was recorded in ½ MS media containing BAP 0.5 mg/l. Response was in the form of single shoot. The single shoots with a mean length of about 1.5mm after one week of growth was obtained. With BAP 2.0 mg/l, single shoots were produced in about 5% of cultures within 54 days. Effect of Kn in various concentration ranging from 0.5 to 2.0 mg/l was found to be low in shoot induction. The maximum length of shoot of about 1.6 cm was recorded in combination of BAP 0.5 mg/l and IAA0.5 mg/l. Various combinations of IAA and IBA at different concentrations were tried for rooting of in vitro shoots. However there was no response in any of the combinations tried.
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    ThesisItemOpen Access
    Pesticide use pattern and monitoring of residues in cardamom in Idukki district
    (Department of Agricultural Entomology, College of Agriculture, Vellayani, 2013) Seena, S M; KAU; Naseema Beevi, S
    The field survey conducted among the farmers of Idukki district revealed that major pest infesting cardamom were shoot and capsule borer and cardamom thrips. For the timely management of these pests, farmers are following strict plant protection measures at an interval of 15 to 40 days with conventional insecticides. Farmers are widely applying heavy doses of chemicals especially the organophosphorus insecticides like phorate, chlorpyriphos, quinalphos, profenophos, methyl parathion and synthetic pyrethroids like cypermethrin and lambda cyhalothrin. Majority of the farmers resort to prophylactic spraying of plant protection chemicals rather than remedial measures. Adoption of IPM strategies are also negligible. Most of the farmers used their own spraying schedules for pest management. The pesticide use pattern in cardamom growing tracts of Idukki district shows that the farmers are applying plant protection chemicals aggressively and the liberal and continual use of pesticides has disturbing consequences on the ecosystem. In multiresidue mehod validation cardamom samples were spiked at five different levels viz. 0.01 µg g-1, 0.05 µg g-1 , 0.10 µg g-1 0.50 µg g-1 and 1 µg g-1 and extraction was carried out using various solvent/ solvent system and the modified QuEChERS method which gave 69.7–110% per cent recovery with RSD < 20 was selected and the same method was adopted for the estimation of pesticide residues from cardamom samples. In order to assess the residue level and to study the extend of contamination due to pesticides in cardamom, samples were collected from the cardamom growing plantations of Idukki district. Three major cardamom growing zones were selected namely Vandanmedu, Udumbanchola and Poopara in Idukki district and ten samples were collected from each location for a period of six months. Data on monitoring of pesticide residues in cardamom samples collected from the study regions for a period of six months revealed varying level of residues of several pesticides. Out of the total 180 samples analyzed, residues were detected in 173 samples and only seven samples were free of residues. Out of the 173 samples detected with pesticide residues, 160 contained multiple residues of pesticides whereas only 13 contained residues of single pesticide. Cardamom capsules contained residues of 16 different pesticide molecules belonging to organochlorines, organophosphates and synthetic pyrethroids. The most common contaminant was quinalphos which was detected in 121 out of 180 samples analysed. Other major contaminants include lambda cyhalothrin (104), cypermethrin (100), chlorpyriphos (87) and profenophos (64). Pesticides detected in cardamom which have no label claim in cardamom include Beta cyfluthrin (5), bifenthrin (3), fenpropathrin (4), fenvalerate (5), lambda cyhalothrin (104), methyl parathion (64) and triazophos (4) . A field experiment was carried out in order to study the curing process on removal of residues of quinalphos, chlorpyriphos, triazophos, cypermethrin, lambda cyhalothrin and imidacloprid. Curing process removed the residues of pesticides at varying levels. Processing factor was worked out for each chemical. Extent of removal of residues as a result of curing were: quinalphos (61.78-67.78%), chlorpyriphos (70.23-76.66%), triazophos (49.62-55.02%), cypermethrin (65.71-67.63%), lambda cyhalothrin (13.15-40.00%) and imidacloprid (75.56-77.32%).
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    ThesisItemOpen Access
    Evaluation of wood quality of selected tropical pines raised in the high ranges of Kerala, for pulp and paper making
    (Department of Tree Physiology and Breeding,College of forestry,Vellanikkara, 2010) Ajayghosh, V; KAU; Anoop, E V
    A study entitled “Evaluation of wood quality of selected tropical pines raised in the high ranges of Kerala, for pulp and paper making” was conducted in the College of Forestry, Kerala Agricultural University, Vellanikkara, Thrissur during the period 2008-2010. The objective of the study was to evaluate the wood quality of Pinus caribaea Morelet, Pinus patula Schl. et Cham. and Pinus oocarpa Schiede grown in research trials of the Kerala forest department in the high ranges of Idukki district of Kerala. Increment core wood samples were collected at breast height from trees, selected at random, from each plot representing each species belonging to different age levels. These samples were then subjected to intensive investigations to find out radial variation (pith, middle and periphery), species variation and influence of age on different wood physical, anatomical and chemical properties. The study revealed that many of the characters studied were influenced by species and age interaction. Radial variation was also found to have significant difference within the species for characters studied. As a whole, under the present climatic condition P.caribaea was found to perform better with wood properties within the accepted range suitable for pulping and paper making. On the other hand, P. patula and P. oocarpa were also found to be promising species for pulping and papermaking with better derived fiber ratios. However, P. oocarpa had specific gravity value slightly more than that is recommended for pulp and paper making. Studies on chemical composition revealed that P. ooccarpa had better performance with higher cellulose and lower lignin content. So what needs to be determined is the relationship, if any, between wood and tracheid properties of these species and the products which can be manufactured from this renewable resource. For this, more extensive study is needed for which the results of this study could be used as a base line data for future tree improvement aspects of these species with reference to wood quality and to bring out their potential utility for future afforestation programmes and timber utility.
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    ThesisItemOpen Access
    Organic nutrition for soil health and productivity of chilli (Capsicum annuum L.)
    (Department of Soil Science and Agricultural Chemistry,College of Agriculture,Vellayani, 2011) Lekshmi, V; Ushakumari, K
    An investigation was carried out at the Instructional farm, attached to the College of Agriculture, Vellayani to evaluate the effect of two enriched composts viz. BM compost and EM compost at different rates alone as well as in combination with two growth promoters viz, Panchagavya and vermiwash on physico-chemical and biological properties of soil and also their impact on crop performance using chilli variety Vellayani Athulya as test crop. The results from the field experiment revealed that the plant growth characters viz. height of the plant at 30, 60 and 90 DAT and primary branches per plant at 90 DAT showed significant variation due to the application of 75% N as BM compost along with Panchagavya. The treatments did not significantly affect the biometric observation, ‘days to 50% flowering’. Significant differences were observed among yield attributing characters viz. number of fruits per plant, fruit length (cm), fruit girth (cm), fruit weight (g), green fruit yield per (g plant-1), total fruit yield (t ha-1) and total dry matter yield (t ha-1). The treatment T8 (75% N as BM compost + Panchagavya) recorded the highest values for all these characters but it was found to be on par with T7 (75% N as EM compost + Panchagavya). This shows that BM compost can be substituted with EM compost when used along with Panchagavya. With respect to quality characters, the treatment T8 (75% N as BM compost + Panchagavya) registered the highest values for capsaicin, ascorbic acid and shelf life of fruits. However ‘oleoresin’ was not influenced by different treatments. Disease incidence percentage (Colletotrichum fruit rot) was found to be reduced by the treatments with organic sources. The treatment T8 (75% N as BM compost + Panchagavya) reduced the incidence of pests and diseases to a considerable extent. Highest BC ratio also was recorded by the same treatment T8 (75% N as BM compost + Panchagavya). Post harvest analysis of the soil indicated that organic carbon, available macro and micro nutrients pH, EC and microbial load and dehydrogenase activity were increased by the combined application of enriched manures viz. 75% N as BM compost and Panchagavya or 75% N as EM compost and Panchagavya. Bulk density was found to be reduced favourably. From the results it was clearly indicated that 75% N as BM compost and Panchagavya or 75% N as EM compost and Panchagavya was superior to all other organic sources in promoting soil health and yield and quality of chilli.
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    ThesisItemOpen Access
    Site specific nutrient management for chilli (Capsicum annum.L) in kalliyoor panchayath of kerala
    (Department of Soil Science and Agricultural Chemistry,College of Agriculture,Vellayani, 2011) Priya, U K; KAU; Sudharmai Devi C R
    Site Specific Nutrient management is a technology in precision fanning that offers chance for farmers to achieve the targeted yield taking into consideration the potential yield of the crop by application of apt amount of fertilizers. The technology is farmers' friendly, eco-friendly and also consumer friendly. In Indian scenario wherein farmers are suffering from yield losses up to 40% due to micronutrient deficiency of soils mainly zinc and boron deficiencies are the yield limiting factors of production. Kerala the soils are low in basic ions that are posing serious threat to crop production. The Judicious application of chemical fertilizers along with micronutrients is necessary for sustainable crop production. The present study "Site Specific Nutrient Management in" Chilli (Capsicum annuum.L.) in Kalliyoor Panchayath Of .Kerala" was carried out to satisfy the objectives viz. study the spatial variability of area, to find outtheindigenous nutrient supply via omission trials, fix a target yield based on the potential yield of the crop and formulate a site specific nutrient recommendation to obtain the targeted yield. In order to understand the spatial variability survey was conducted at 25 sites of Kalliyoor panchayath. The results of survey showed that the soils showed wide variation in terms of soil physical and chemical properties. Soil phosphorus status , was high in all cases other nutrient concentration ranged from low to high. The spatial variability necessitated carrying out the omission trials at four different sites that were ranked according to the nutrient status into high, medium first level, medium second level and low fertility soils. From the omission trials the recovery fraction and, indigenous nutrient supply were calculated. Utilising all these parameters in QUEFT model SSNJ\:1.--tre tments were fixed. /' / / - :l.33- The high yield target was fixed at 16 t ha that was 50% of the potential yield. The nutrient recommendation generated for HYT was 104.8: 13.6: 201 kg N, P205 and K20 ha-I. Medium yield target was fixed at 168:51: 230 kg N, P205 and K20 ha- I. Taking into account micronutrient and secondary nutrient deficiencies in the soil. In high yield target soil application of micro nutrients and secondary nutrients along with application of 1 % foliar spray of boron was recommended. Micronutrients for soil application were boron @ 5 kg ha-I in form of borax, zinc @ 20 kg ha-I in form of zinc sulphate. Secondary nutrients applied were calcium @ 30 kg ha-I in form of CaS04, magnesium @ 7.5 kg ha-I in form of MgS04. The requirement of sulphur was met from soil applied zinc sulphate, calcium sulphate and magnesium sulphate. In medium yield target only soil application of micro and secondary nutrients along with the application of QUEFT generated recommendations for the crop; POP + micronutrient recommendations were evaluated. Simultaneously an absolute control was also carried out. Soil analysis was carried out for all the physical and chemical properties of the soil initially before the crop was raised, and after each harvest. So that change in soil properties as a result of application of treatments could be evaluated, since the application of fertilizer coincided with each harvest. The results derived from the experiment proved the superiority of SSNM over other treatments with respect to the yield major nutrient and micronutrient uptake. , Where high yield target registered a cumulative yield of 17. 32 tons, medium yield target registered a cumulative yield of 11.75 tons this was _much superior to POP+SNMN that registered an yield of 9.83 tons, or the package of practice recommendation that registered an yield of only 8.3 tons . Farmers practice registered inferior yield data of only 5.2 tons. The study revealed that the site specific nutrient management is an efficient technology to increase the yield of crops and hence provide additional income to the farmers; this technique also provides a -134 - scope of increasing the yield without over application of fertilizers that would result in deterioration of the soil physical and chemical properties in long run.
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    ThesisItemOpen Access
    Hepatoprotective effect of azadirachta indica (NEEM) and tridax procumbens (CHIRAVANAK) in rats
    (Department of Veterinary Pharmacology and Toxicology,College of veterinary and animal sciences,Mannuthy, 2010) Ranjana Mooken, KAU; Aravindakshan, C M
    The objectives of the present study were to evaluate the hepatoprotective activity of ethanolic extract of Azadirachta indica and Tridax procumbens leaves in paracetamol induced hepatic damage in rats and to compare their action. Forty adult male wistar rats weighing 150-200 g, divided into five groups comprising eight animals in each group, were used for the study. Groups I and II animals were administered with 3 per cent gum acacia suspension in distilled water at the dose rate of 5 ml/kg/day for ten days. Groups III and IV animals received ethanolic extract of Azadirachta indica and Tridax procumbens leaves at the dose of 300 mg/kg and Group V animals received reference drug silymarin at the dose of 100 mg/kg/day in 3 per cent gum acacia for ten days. All the groups except the group I, received paracetamol orally on the eighth day at the dose rate of 2 g/kg in distilled water. Blood was collected from all the groups before and after the experiment for various biochemical and haematological parameters. Body weight was recorded on day 0 and 10th day. All the animals were sacrificed on 10th day and liver was taken for histopathological examination. Phytochemical analysis of the Azadirachta indica (Neem) leaf extract revealed the presence of alkaloids, tannins, flavonoids, glycosides, phenolic compounds, diterpenes, triterpenes and saponins and that of Tridax procumbens (chiravanak) showed the presence of alkaloids, steroids, glycosides, flavonoids, saponins and tannins. Paracetamol administered group showed a decrease in body weight. Liver marker enzymes like ALT and AST were highest in paracetamol treated groups. The elevated levels of these enzymes were decreased by the herbal extracts thereby proving their hepatoprotective activity. A reduction in the total protein was observed in the paracetamol treated group. Both the herbal extracts elevated the total protein level to normal levels. Serum albumin level was also lowest in the paracetamol treated group because of the decrease in the total protein level. Both the extracts increased the serum albumin levels towards normal values. Serum bilirubin level was also highest in the paracetamol treated group Azadirachta indica and Tridax procumbens leaf extract at the dose rate of 300 mg/kg decreased the elevated level of bilirubin. The haematological parameters showed not much significant change with the treatment. From the biochemical studies it was noted that the administration of Azadirachta indica (Neem) leaf extract caused better reduction in serum parameters than that of Tridax procumbens extract. Gross examination of the liver showed normal appearance in all the four groups except the paracetamol treated group in which the liver showed areas of coagulative necrosis and congestion. On histopathological examination the paracetamol treated group showed extensive areas of centrilobular coagulative necrosis. The Azadirachta indica treated animals showed diffuse necrotic areas and fatty changes in certain lobules. The areas of regeneration with binucleate hepatocytes were abundant in this group. The Tridax procumbens treated group showed hypertrophied hepatocytes with vacuolation of cytoplasm and presence of fat droplets. The areas of regeneration with binucleate hepatocytes were scanty in this group. Silymarin treated animals have almost normal liver architecture like the control group. From the present study it can be concluded that the ethanolic extracts of leaves of A.indica and T.procumbens have significant hepatoprotective activities in paracetamol induced hepatotoxicity in rats and A.indica has a better hepatoprotective action compared to T.procumbens.
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    ThesisItemOpen Access
    Standardization of processing methods for production of quality white pepper.
    (Department of Processing Technology,College of Agriculture,Vellayani, 2011) Shameena Beegum, P P; KAU; Vasanthakumar, K
    The research entitled “Standardization of processing methods for production of quality white pepper”was conducted at the Department of Processing Technology, College of Agriculture, Vellayani . The study was undertaken with the objective of producing good quality white pepper using improved processing methods. The experiment was divided into three parts viz., chemical method of white pepper production, microbial fermentation method and organoleptic evaluation of the resultant products. The experiment was laid out in CRD with three replications. Seven promising bleaching agents were selected for the chemical experiment. Berries with calcium hypochlorite, calcium hydroxide, hydrogen peroxide, sodium hypochlorite, sodium hydroxide, sodium hydroxide, benzoyl peroxide and the conventional retting (control) constituted the eight treatments. Effects of these treatments on the physical and chemical properties of white pepper berries were analysed. All the physical properties except sizes of berries were significantly influenced by the treatments. Treatment with sodium perborate recorded maximum specific gravity followed by sodium hypochlorite, hydrogen peroxide. The highest value for 1000 berry weight was reported from berries treated with sodium hypochlorite. Treatment with calcium hypochlorite and sodium hypochlorite had registered a significantly higher value for 1000 berry volume. Calcium hypochlorite treated berries were having the highest yield. Berries produced by conventional retting process (control) found to be superior in volatile oil, oleoresin, piperine and non volatile ether extract and treatment with calcium hypochlorite was on par with control in all chemical parameters. Residual level of bleaching agents was found to be in a safe range for consumption. The microbial fermentation work was consisted of, isolation of organisms through enrichment culture technique, evaluation of available cultures and standardization of retting process for white pepper production. Seven isolates of bacteria IsB1 to IsB7 and eight fungal isolates IsF1 to IsF6 were obtained. Evaluation of isolates was done by assessing the pectinolytic and cellulolytic activities. The isolates IsF1 and IsF3 were also capable for degrading pectin. Degradation of cellulose was maximum in IsF3, Ay1 and Mycophyta. Bacterial isolates did not produce any zone for both asparagine and MP-5 media. Compared to bacterial isolates fungal isolates was faster in retting. Isolate IsF3, Ay1and Mycophyta were the most promising and recorded 100 percentage retting without any damage by third day itself. Amongst all the isolates, considering the retting process and quality of the product, the isolates IsF3 and Ay1 were found to be the most superior. Berries treated with sodium perborate recorded maximum over all acceptability for Organoleptic qualities eventhough it resulted poor chemical qualities. Amongst all the eight treatments, considering quality parameters and quality of developed product, treatment with calcium hypochlorite was found to be the most superior. The above study could be concluded with the findings that, prolonged retting period can be shortened to three to four days using microbial fermentation method with promising isolates IsF3 and Ay1. While opting for a better colour, bleaching with a promising chemical bleaching agent like calcium hypochlorite (0.2%) can be recommended.
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    ThesisItemOpen Access
    Reliability of united score card for crossbred dairy cows of Kerala
    (Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2010) Dhanya, K; KAU; Anil, K S
    The research work was conducted to assess the reliability of Unified score card for cross bred cows of Kerala. One hundred and ninety six animals of University cattle farms of Mannuthy, Thumbunnuzhi and Thiruvazhamkunnu were selected for the study. As per the Unified score card by Purebred Dairy Cattle Association, the animals were judged for frame, dairy character, body capacity, feet• and legs and udder. The type components were again subdivided as described in the scorecard to improve the accuracy of judging score. The body measurements of individual animals like height at withers, body length, chest girth, height at withers, height at pins, length of quarter, width between angles of hook and slope of rump were taken. The animals were categorized into four age groups, four parities and three stages of lactation. The emphasis of each of the components of unified score card to milk production was compared and statistical analysis was carried out as per methods suggested by Snedcor and Cochran (1994). Effects of age, stage of lactation and parity on judging using Unified Score Card were worked out. The body measurements were also correlated with the score and milk yield. Age, parity and stage of lactation were found to be nonsignificant for final score. The scores of frame, dairy character and udder for different age groups showed no significant difference, whereas, scores of body capacity and feet and legs differed significantly. The scores of dairy character and body capacity differed significantly with parity. No significant difference was shown for scores of frame, feet and legs and udder. Frame was found to be positively correlated with other components, the least being with udder (r=0.127) and highest with feet and legs (r =0.254) which was highly significant (p<0.01). Dairy character was significantly correlated with body capacity (r=0.169) and feet and legs(r=0.2S4) in addition to frame. Feet and legs was the only component to which udder showed positive significant correlation (pAge was found to be not significant for lactation yield (P~O.OS). The means of lactation yield differed significantly for different parities. All the type components were found to be positively correlated with lactation yield, all being significant. The standardized partial regression coefficients were highly significant for dairy character, udder. and body capacity whereas non significant regression coefficients were obtained for frame and feet and legs. Udder depth was having the highest regression coefficient (~=0.312) which was highly significant (p<0.01). Regression coefficients were significant and positive for skin (0.229), withers (0.188), ribs (0.174) and thighs (0.162). Both age and parity had significant positive correlation on body weight. Body weight was significantly (p<0.0 1) correlated with lactation yield with r=0.244. Height at withers, length, hip width girth and height at hook showed significant difference with age (p:SO.OS).The height at pins and slope were found to be not affected significantly by age. All the measurements except slope were significant and positive in correlation with body capacity. All height measurements, length and girth was found to be positively correlated with frame (p<0.0 1). Final score was having significant positive correlation with all height measurements (p:SO.OS). It was concluded that although the score card was found to be reliable on the production of crossbred cows of Kerala, the emphasis placed on type components was not in full agreement with what was prescribed by the purebred Dairy Cattle Association Dairy Cow Unified Score Card. Hence it was suggested that more weightage could be given to body capacity and dairy character compromising weightage on frame and feet & legs which necessitated further studies in this regard.