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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Standardisation of spacing and nutrient levels for fodder rice bean [Vigna umbellata (Thunb.)].(Department of Agronomy, College of Agriculture, Vellayani, 2018) Ajmal Fayique, C; KAU; Usha C, ThomasThe study entitled “Standardization of spacing and nutrient levels for fodder rice bean [Vigna umbellata (Thunb.)]” was conducted at College of Agriculture, Vellayani, Kerala during Kharif 2017 to standardize the spacing and nutrient requirement of fodder rice bean and to study its impact on growth, yield and quality of the crop. The experiment was laid out in Randomised Block Design (33 confounded factorial) with three replications.The treatments consisted of three spacings (s1 - 30 cm x 10 cm, s2 - 30 cm x 20 cm and s3 - 30 cm x 30 cm), three levels of nitrogen (n0 - 0 kg ha-1, n1 - 20 kg ha-1 and n2 - 30 kg ha-1) and three levels of phosphorous (p0 - 0 kg P2O5 ha-1, p1 - 20 kg P2O5 ha-1 and p2 - 40 kg P2O5 ha-1) . FYM @ 5 t ha-1 and K2O @ 30 kg ha-1 were applied uniformly to all treatments as basal. The treatment s1 resulted in the highest plant height at 30 DAS and leaf: stem ratio at harvest. Application of N @ 20 kg ha-1 registered the highest plant height and was on par with 30 kg N (n2) while leaf stem ratio was the highest at n2. Levels of P had no significant impact on growth characters. The treatment combination s2n2p1 produced the tallest plants (173.17 cm) at harvest and treatments s1n0p1 and s1n2p2 recorded the highest leaf: stem ratio (0.82) but were on par with s1n0p0, s2n0p0, s n0p2, s1n1p0, s2n0p2 and s3n0p1. At 30 DAS, s1 produced the highest LAI (2.27) while at harvest, s2 was found superior. The highest NAR was observed at s1 and was on par with s3. Closer spacing (s1) enhanced the CGR at 30 DAS and harvest. Application of 30 kg N ha-1 (n2) enhanced LAI at both stages. At 30 DAS and at harvest, higher NAR were observed at n1 and n2. At 30 DAS, n2 and p1 registered the highest chlorophyll contents. The treatment s1 n2 p1 (30 cm x 10 cm spacing + 30 kg N ha-1 + 20 kg P2O5 ha-1) resulted in the highest LAI, CGR and chlorophyll content at 30 DAS. Spacing and N levels had significant impact on green fodder yield (GFY) and dry fodder yield (DFY). The highest GFY (12.95 t ha-1) and DFY (2.59 t ha-1) were produced at s1 (30 cm x 10 cm) and was on par with s2. The highest GFY (13.66 t ha-1) and DFY (2.73 t ha-1) were produced at n2 (30 kg N ha-1) and was on par with n1. The S x N x P interaction s1 n2 p1 (30 cm x 10 cm + 30 kg N ha-1 + 20 kg P2O5 ha-1) recorded highest GFY (17.29 t ha-1) and DFY (3.46 t ha-1). The different spacing had no impact on crude protein (CP) but the lowest crude fibre (CF) was observed at s1. Application of 30 kg N ha-1 (n2) resulted in the highest CP content and the lowest CF content was estimated at 0 kg N ha-1. Among P levels, p2 recorded the highest CP (17.69%) and was on par with p1. The lowest CF (16.43 %) was observed at s2n0p1 (30 cm x 20 cm spacing + 20 kg P205 ha-1) and was on par with s1n0p0, s1n0p1, s1n2p0, s2n0p0, s2n0p2, s3n0p0 and s3n0p1. No variation in N uptake was observed due to treatments. Uptake of P varied with N levels only and n1 and n2 recorded the highest P uptake. Spacing and P levels influenced K uptake by the crop and the highest uptake was observed at s1 and p2 but p2 was on par with p1. The three factor interaction s1n2p1 registered the highest P and K uptake. However, it was on par with s1n1p2, s1n1p0, s2n1p1 and s3n0p2 in P uptake and with s1n2p2 in K uptake. Increasing N levels increased pH and EC of soil after the experiment. Soil available N after the experiment was the highest at s3 (on par with s2) and n2 (on par with n1). At wider spacing, application of N enhanced the availability of N in the soil after the experiment. Available P in the soil varied with S x P interaction but all treatment combinations were on par except s2p0 and s3p2. The highest soil available K was observed at n0 among N levels and at p1 among P levels. The interactions S x N, S x P and N x P significantly influenced available K in the soil. Economic analysis revealed the highest net income (₹ 35762) and BC ratio (3.22) at s1n2p1 (30 cm x 10 cm spacing + 30 kg N ha-1 + 20 kg P2O5 ha-1). From the study, it can be concluded that fodder rice bean can be profitably cultivated at a spacing of 30 cm x 10 cm with application of 30 kg N ha -1 in two splits at 15 and 30 DAS and basal application of 20 kg P2O5 ha-1, 5 t ha-1 of FYM and 30 kg K2O ha-1.ThesisItem Open Access Evaluation of miRNA prediction tools and in silico analysis of micro and long non coding RNAs in sweet potato(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2018) Aswathy, M B; KAU; Sreekumar, JThe study entitled “Evaluation of miRNA prediction tools and in silico analysis of micro and long non coding RNAs in sweet potato (Ipomoea batatas L.)” was conducted at the ICAR-CTCRI, Sreekariyam. The objectives of the study is to compare different miRNA and target prediction tools and in silico analysis of the miRNAs and lncRNAs in sweet potato. The plant miRNA identification tools: NOVOMIR and miRPlant and miRNA-target prediction tools: psRNATarget and miRanda were compared. NOVOMIR and psRNATarget were found to be a better tool in miRNA identification and target prediction. MicroRNAs (miRNA) are 18-22nt small, endogenous non coding RNA that has prominent role in many biological processes. In the present study, we report the computational prediction of miRNAs and targets from expressed sequence tags (ESTs) of sweet potato. We predicted 13 novel potential miRNAs and 81 potential target genes and functionally characterized by BLASTX and BLAST2GO. The predicted target genes were credited with their role in signalling cascades, metabolism, and defence and stress responses. Another candidate that has more importance in the genome regulation is lncRNAs. lncRNAs are greater than 200 nucleotide length ncRNA candidate that holds functions at RNA level itself. RNAplonc is a plant long non coding RNA identification tool which uses 16 feature selection methods to predict long non coding RNA molecules. The present study which predicts 9215 lncRNAs and 8665 protein coding genes by RNAplonc in sweet potato for the first time using available ESTs sequences. Since there is a lack of lncRNA functional annotation tool, the functional analysis of predicted lncRNAs is quiet difficult. From the predicted miRNAs and lncRNAs two miRNAs and two lncRNAs were randomly selected for experimental validation by real time quantitative PCR using three different sweet potato varieties Sree Kanaka, ST13 and Khanjakad available at ICAR-CTCRI and compared the target gene’s expression in each variety. Validation results prove that both the miRNAs and lncRNAs shows their importance in crop improvement.ThesisItem Open Access Effect of heat stress on the expression patterns of different growth related genes in Malabari goats(Academy of Climate Change Education and Research, Vellanikkara, 2018) Angel Sunny, P; KAU; Bagath, MThe impact of heat stress on growth performance of goats has been established fairly based on changes associated with phenotypic traits. However, not many reports are available on the genotypic traits which get altered on exposure to heat stress in livestock. Therefore, the study is an attempt to elucidate the molecular mechanisms governing growth performance during heat stress in goats. The primary objective of the study was to establish the influence of heat stress on the expression patterns of different growth related genes in Malabari goats. The study was conducted for a period of 45 days in twelve Malabari goats randomly allocated into two groups: MC (n=6; Malabari control) and MHS (n=6; Malabari Heat stress). Goats were stall-fed with a diet composed of 60% roughage and 40% concentrate. All animals had access to adlibitum feed and water and they were fed and watered individually. The MC goats were placed in the shaded pens while MHS goats were exposed to heat stress in outside environment between 10.00 h to 16.00 h. At the end of study period, all 12 animals were slaughtered and their liver tissues were collected for gene expression and histopathological studies. The temperature-humidity-index (THI) inside the shed (74.9) proved that the animals were not stressed while in the outside environment (86.5) the animals were extremely distressed. The hepatic growth hormone (GH), growth hormone receptor (GHR), insulin-like growth factor-1 (IGF-1), leptin (LEP) and leptin receptor (LEPR) gene expression patterns were significantly (P<0.05) lower in heat stress group as compared to the control group animals. In addition, negative correlation (P<0.05) was also established between THI and all the growth related gene expression in the study. The hepatic histopathological section showed more fatty and degenerative changes (P<0.05) in hepatocytes in MHS group as compared to MC group. The study offers the first thorough insight into the expression patterns of different growth related genes during heat stress exposure in goats. Further, the study established GH, GHR, IGF-1, LEP, LEPR genes to be the ideal markers to reflect growth potential in Malabari goats. The findings from this study provide some crucial initial information on how different growth relatedgenes are expressed when Malabari goats are subjected to heat stress. This information might be of high value in assessing the growth performance of goats and may provide useful information pertaining to nutrient supplementation to the heat stressed goats.ThesisItem Open Access Identification of water stress tolerant amaranthus genotypes (Amaranthus tricolor L.) with high yield and quality(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Shahiba, A M; KAU; Beena ThomasThe present study entitled “Identification of water stress tolerant amaranthus genotypes (Amaranthus tricolor L.) with high yield and quality” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2016-2018, with an objective to identify high yielding genotypes of amaranthus with good quality and tolerance to water stress. The study was conducted under two experiments. In the first experiment thirty accessions of Amaranthus tricolor L. available in the Department of Plant Breeding and Genetics and collected from other sources were evaluated for yield under field condition and morphologically described using IBPGR descriptor for the amaranthus. Madhur local (A22) recorded highest yield plant-1(125.926g) followed by Kalliyoor local (A4), Ayyanthole local (A28), Haripad local (A7), Palakkadu local (A2), Anachal local (A6), Aryanadu local (A21), Poonkulam local (A20), Kazhakkuttom local (A9) and Kannara local (A29). In the second experiment, these ten genotypes selected based on the yield were evaluated in a Randomized Block Design (RBD) with three replications during November 2017- December 2017. Water stress was imposed after 5 days of irrigation to water holding capacity to the transplanted seedlings by scheduling the irrigation at a depth of 20mm at 20mm CPE (Cumulative Pan Evaporation). Analysis of variance was calculated for all the characters under study and was found to be significant for all the genotypes evaluated. The mean performance of the genotypes for the characters were studied. The maximum yield was observed for the genotype A22 (Madhur local) followed by the genotype A9 (Kazhakkuttom local), genotype A20 (Poonkulam local) and genotype A2 (Palakkadu local) and the minimum yield was recorded for genotype A4 (Kalliyoor local). The genotype A22 (Madhur local) showed the highest mean values for stem girth, number of branches, length of leaf lamina, leaf to stem ratio, membrane integrity, relative water content, proline content of leaves, vitamin A and lowest oxalate content. The character Vitamin A content registered the highest GCV (41.22%) and PCV (41.25%). High heritability coupled with high genetic advance was observed for leaf width, number of branches, yield plant-1, protein content, fibre content and vitamin A. The yield plant-1was found to be significantly and positively correlated with leaf width, number of branches, yield plot-1, membrane integrity, proline content of leaves and vitamin A both at genotypic and phenotypic levels. Petiole length and percentage leachate were found to be negatively correlated with yield plant-1. Path analysis revealed that number of branches, yield plot-1and proline content of leaves had the maximum positive direct effect on yield plant-1. The results of the present study showed that genotype A22 (Madhur local) was superior in yield performance under water stress condition followed by the genotype A9 (Kazhakkuttom local), genotype A20 (Poonkulam local) and the genotype A2 (Palakkadu local). The genotype A22 (Madhur local) also recorded the maximum stem girth, number of branches, length of leaf lamina, leaf to stem ratio, membrane integrity, relative water content and proline content of leaves with high Vitamin A and low oxalate content. Presence of proline in the leaves might be considered as an important water stress tolerance mechanism.ThesisItem Open Access Weed management in upland rice (Oryza sativa L.) intercropped in coconut(Department of Agronomy, College of Agriculture, Vellayani, 2018) Ravikiran; KAU; Elizebeth Syriac, KThe investigation entitled “Weed management in upland rice (Oryza sativa L.) intercropped in coconut” was undertaken during the period, 2017 - 2018 at College of Agriculture, Vellayani, Thiruvananthapuram to standardise an eco-friendly and economic weed management strategy for upland rice intercropped in coconut. The field experiment was carried out at Coconut Research Station, Balaramapuram, Thiruvananthapuram district during the period from June to October 2017. The variety used was Prathyasa (MO-21) released from Rice Research Station, Moncompu. The experiment was laid out in randomized block design (factorial) with sixteen treatment combinations and three replications. The treatments consisted of two stale seedbed methods viz., stale seedbed with mechanical removal of weeds (s1) and no stale seedbed (s2) and eight weed management methods i.e., penoxsulam @ 20 g ha-1 at 10-15 days after sowing (DAS) fb hand weeding (HW) at 35-40 DAS (m1), penoxsulam @ 25 g ha-1 at 10-15 DAS fb HW at 35-40 DAS (m2), penoxsulam @ 30 g ha-1 at 10-15 DAS fb HW at 35-40 DAS (m3), penoxsulam @ 20 g ha-1 at 10-15 DAS fb metsulfuron methyl + chlorimuron ethyl (MM+CE) @ 4 g ha-1 at 35-40 DAS (m4), penoxsulam @ 25 g ha-1 at 10-15 DAS fb MM+CE @ 4 g ha-1 at 35-40 DAS (m5), penoxsulam @ 30 g ha-1 at 10-15 DAS fb MM+CE @ 4 g ha-1 at 35-40 DAS (m6), HW twice at 15 and 35 DAS (m7) and weedy check (m8). Study of the weed flora of experimental area indicated the dominance of broad leaved weeds (eight spp.) followed by grasses (six spp.) and sedges (two spp.). Stale seedbed method (s1) recorded significantly lower weed density at all stages of observations (15, 30 and 60 DAS), weed dry weight at 15 and 30 DAS and higher weed control efficiency (WCE) at 30 and 60 DAS compared to no stale seedbed. Among the weed management methods, at 60 DAS, all the penoxsulam doses i.e., 20, 25 and 30 g ha-1 at 10-15 DAS fb either HW at 35-40 DAS or MM+CE at 35-40 DAS (m 6, m4, m3, m5, m1 and m2) were on par in their effect on total weed dry weight and WCE. Stale seedbed method (s1) registered significantly higher plant height at 60 DAS, number of tillers m-2 at 30 DAS and at harvest, dry matter production at harvest and LAI at 60 DAS compared to no stale seedbed (s2). Penoxsulam at different doses fb HW treatments (m 1, m2 and m3) recorded higher plant height at 30 and 60 DAS and at harvest, number of tillers m-2 at 30 DAS, DMP at harvest and LAI at 30 DAS. None of the herbicide treated plots showed any phytotoxicity symptom on rice crop. The yield attributes viz., number of spikelets panicle-1, per cent filled grains and thousand grain weight were significantly higher for stale seedbed, compared to no stale seedbed (s2). Weed management methods also significantly improved yield attributes viz., panilces m-2, spikelets panicles-1, per cent filled grains and thousand grain weight compared to weedy check treatment. Penoxsulam @ 25 g ha-1 at 10-15 DAS fb HW at 35-40 DAS (m2) registered the highest values for per cent filled grains and thousand grain weight. Stale seedbed method (s1) recorded significantly higher grain yield, straw yield and lower weed index compared to no stale seedbed method (s 2). Though penoxsulam @ 25 g ha-1 at 10-15 DAS fb HW at 35-40 DAS (m2) registered the highest grain yield (3.23 t ha-1), it was on par with the other penoxsulam fb HW treatments viz., m1 and m3. None of the s x m interactions were found statistically significant. Regarding weed index also m2 registered the lowest value and it was significantly superior to all other weed management methods. Higher net income (₹ 27, 848/-) and B:C ratio (1.48) were obtained with stale seedbed method (s1), compared to no stale seedbed (s2). Application of penoxsulam @ 25 g ha-1 at 10-15 DAS fb HW at 35-40 DAS (m2) registered the highest net income (₹ 36,090/-) and B: C ratio (1.63). The s1m2 registered the highest net income (₹ 44,433/-) and B: C ratio (1.77), among the interactions. The results on soil microbial population revealed that population of fungi, bacteria and actinomycetes increased in all the herbicidal treatments compared to pre-treatment population. Activity of dehydrogenase enzyme also increased significantly in the herbicide applied plots implying the safety of the tested chemicals on soil health. Based on grain yield, weed index and economic analysis, integration of stale seedbed method with the broad spectrum herbicide penoxsulam @ 25 g ha-1 at 10-15 DAS fb HW at 35-40 DAS can be recommended as an eco-friendly and economic weed management practice in upland rice.ThesisItem Open Access Novel bioformulations of entomopathogenic fungi and their efficacy aganist banana weevils(Department of Agricultural Entomology, College of Agriculture, Vellayani, 2018) Remya, S; KAU; Reji Rani, O PThe study entitled ‘Novel bioformulations of entomopathogenic fungi and their efficacy against banana weevils’ was carried out at Dept. of Agrl. Entomology, College of Agriculture, Vellayani during 2016-2018, with the objective to develop novel formulations of entomopathogenic fungi, Metarhizium anisopliae (Metch.) Sorokin, Beauveria bassiana (Bals.) Vuillemin and to evaluate their efficacy in managing banana weevils. It was intended to develop capsule and gel formulations. Experiment to standardize a coating material for developing capsules revealed that Hard Gelatin Transparent (HGT), Hard Gelatin Coloured (HGC) and Hydroxy Propyl Methyl Cellulose (HPMC), were equally stable under ambient conditions of storage (26 -33°C and RH 60-80%). On testing their ability to disintegrate under field conditions, it was noted that chitosan filled HGT capsules easily disintegrated at 20% soil moisture after 36 h and after 24 h at 30 % soil moisture. It took 144 h for all capsules to disintegrate in sheath or leaf axil and 24 h in bore holes. Talc and chitosan were superior, in maintaining storage stability as well as degradability. A trial conducted to determine the moisture content of capsules, revealed that 10 % was the ideal moisture level content of the ingredient, to maintain storage stability as well as viability of the formulation. Viability noticed after three months was 2.29 × 107 cfu mL-1 and 2.27 × 107 cfu mL-1 in chitosan based capsules of M. anisopliae and B. bassiana and 2.10 × 107 cfu mL-1 and 0.76 × 107 cfu mL-1 in talc based capsules. Therefore, capsules were developed with HGT coating, with chitosan / talc as carrier at 10 % moisture content. Shelf life studies revealed that chitosan was the best carrier material compared to talc in retaining viability of Metarhizium capsules, with a mean cfu of 2.51 × 107 mL-1 and 1.77× 107 mL-1. During a period of three months of storage, there was no decline in the number of viable colonies, cfu ranging from 1.72 × 107 mL-1 to 2.79 × 107 mL-1. . Storage under refrigeration had better retention of viability (2.63 × 107 cfu mL-1) than at room temperature (1.64× 107 cfu mL-1). In Beauveria capsules, the viability did not differ in both chitosan and talc based capsules. Here also, the number of viable colonies did not decline statistically, till three months of storage, with mean cfu values ranging from 1.85 × 107 mL-1 to 2.36 × 107 mL-1. In general, shelf life of capsules could be extended by two months when chitosan was used as the carrier material. Low temperature storage could also improve the shelf life by two months. Shelf life studies of gel formulations indicated that both Metarhizium and Beauveria gels exhibited high viability in chitosan at room temperature and under refrigeration. The mean number of viable colonies observed was 2.39 × 106 cfu mL-1 in chitosan based gels of Metarhizium and 2.17 × 106 cfu mL-1 in Beauveria gels. The number of viable colonies of Metarhizium and Beauveria observed throughout the experimental period did not vary significantly over three months of storage. The mean number of cfu being 2.26 × 107 mL-1 on the 15th day and 1.41 x 106 mL-1 on the 90th day . It was also inferred that chitosan when used for formulating gels could extend the shelf life of both organisms by one month. Pathogenicity test disclosed the affinity of M. anisopliae to rhizome weevil and B. bassiana to pseudostem weevil. Pot culture studies to evaluate chitosan and talc based capsules of B. bassiana revealed that both the capsules of Beauveria were effective as chlorpyriphos 20 EC 0.05% for pseudostem weevil, in prophylactic and curative methods. The damage index (DI) was one each and reduction in pest population was 91.67 % in prophylactic control and 91.67-100 % in curative control. Metarhizium capsules reduced the damage caused by rhizome significantly, but the reduction in pest population was only to the tune of 47-55 % in prophylactic and 50- 58 % in curative control. Chitosan based gel of Metarhizium tested against rhizome weevil, reduced the damage significantly (DI 46.67 and 30, in prophylactic and curative methods). The pest population was reduced by 61.11 % in prophylactic and 36.11 % in curative methods. The study could standardize the protocol for capsule and gel formulations of entomopathogenic fungi, retaining the viability and infectivity upto three months of storage. It is concluded that placement of Beauveria capsules in leaf axils prophylactically and curatively can effectively control pseudostem weevil and Metarhizium capsules and gels placed in the rhizosphere could manage the rhizome weevil moderately.ThesisItem Open Access Status and habitat preferences of White-rumped vulture (Gyps bengalensis ) population of Wayand wildlife sanctuary, Kerala(Department of Wildlife Sciences, College of Forestry, Vellanikkara, 2018) Vishnu, M; KAU; Nameer, P OThe research work entitled "Status and habitat preferences of White-rumped Vulture (Gyps bengalensis) population of Wayanad Wildlife Sanctuary, Kerala" was studied during August 2017 to April 2018. It was carried out in four study locations viz., Kurichiat, Muthanga, Sulthan Bathery and Tholpetty. The main objective of the study was to find out the status, nesting characteristics, feeding observations and interview of various stakeholders. Three species of vultures included in the family Accipitridae were reported from the current study in Wayanad WLS viz., Gyps bengalens is, Gyps indicus, and Sarcogyps calvus. Total of 4 active nesting trees were identified from two different colonies this year. All the 4 nest were present in Haldina cardifolia. Out of the 4 nests, 3 nests belongs to Gyps bengalensis located in Kazukankolli and 1 nest belongs to Sarcogyps calvus which was the 1st report of nesting of this species in the sanctuary located in Karamukku, Kurichiyad. Apart from this, 9 nests of the previous years on 6 different tree species were also studied. Other nesting tree species were Terminalia elliptica, Lagerstroemea microcarpa, Dalbergia latifolia, Stereospermum chelenoides and Tectona grandis. Thirty species of trees were identified belonging to 17 different families from the 44 circular plots surveyed in the nesting colony of Wayanad Wildlife Sanctuary. Most of the nesting are having height between 25 m - 35 m and girth more than 300 em. Mostly height of the nests are in between 23 m - 26 m of the nesting trees. IVI of the nesting trees are comparatively higher than the other tree species got from the plots. The vegetation parameters that influence the nesting habitat of vultures are the girth of the tree, height of the tree and the tree species diversity in the area. The camera trapping has been found to be a useful technique for the population estimation of the vultures at the vulture restaurants in Wayanad. The camera trap success rate of vultures observed in Kakkappadam is 34.09%. The high success rate is because it is a dumping ground of carcass. Carcass monitored was of Elephant, Sambar Deer and Spotted deer. The most preferred feeding hours by the vultures were 7-8 hours and 12-13. hours.From the survey conducted among the veterinarians and the medical shops it was found that the harmful NSAID's such as Ketoprofen are being used to treat livestock, which could be a problem for the long-term conservation of the vultures. The use of the veterinary diclofenac was nil at Wayanad District. However, some of the medical practitioners were found using the human diclofenac for treating livestock.ThesisItem Open Access Comparative evaluation of tools for gene regulatory network prediction and network reconstructioon using genomic data(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2018) Reshma Bhasker, T; KAU; Sreekumar, JDeveloping regulatory network of genes controlling traits which are of importance economically and commercially are gaining much significance in present times. GRN’s provide an insight into the transcriptional mechanisms that regulate the robust and stochastic gene expression and their relationship with the phenotypic variability that can be utilized for better crop improvement strategies. The former approaches for Gene Regulatory Network construction mainly rely on using gene expression data as input, but the time consumption and high cost of expression analysis paved way for developing new methodologies that make GRN prediction easier. The integration of genomic information along with gene expression data, could make the process of Gene Regulatory Network (GRN) construction more reliable than using expression data alone as input source. Using this approach, we have tried to develop the regulatory network of genes controlling immunity in cassava with special context to Bacterial blight resistance. Initially the immunity related genes in cassava were identified by protein domain search and analysis using HMMER. Cassava specific genes were further filtered for high competency, mapped and annotated to determine its biological role and function. A set of 1919 immunity related genes in cassava were identified, out of which 22 of them were specifically conferring virus resistance, 727 of them were screened for bacterial blight resistance by microarray data integration and a network was created using they predicted interactions identified from 324 genes using STRING. The networks obtained was visualized using Cytoscape and cross validated with simulated dataset generated from SynTReN. The generated network if immunity related genes in cassava could give more insight into the defence mechanism in cassava that can help in adapting better crop improvement and management strategies. A comparison of various approaches used for GRN prediction like probabilistic method, mutual information-based method, correlation-based approaches etc was also done and various tool like ARACNE, WGCNA etc were evaluated. Networks with different sizes, 50, 100 and 150 was generated and the network parameters like clustering coefficient, network density etc were compared. Clustering coefficient does not seem to vary with increase in network size but network heterogeneity and density were observed to increase. The statistical analysis of the performance of different methods resulted into a conclusion that the mutual information based approaches are better tools for Gene Regulatory Network construction than the other methods and it performed with a specificity of 75.7% and a sensitivity of 79.4%.ThesisItem Open Access Assessment of antiinflammatory and antioxidant properties of chlorophytum laxum R.Br.(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2018) Arundhathy, G B; KAU; Suja, S RThe thesis entitled ‘Assessment of antiinflammatory and antioxidant properties of Chlorophytum laxum R. Br.’ was carried out in the Ethnomedicine and Ethnopharmacology Division of Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram during the academic year 2017-2018. The objective of the study was to scientifically evaluate antiinflammatory and antioxidant properties of an ethnomedicinal plant Chlorophytum laxum R. Br. (Neeruvatti). Chlorophytum laxum R. Br. (Neeruvatti), herbaceous plant of family Liliaceae is one of the important medicinal plant seen in grasslands. The tubers of Chlorophytum laxum R. Br. were collected from the hills of Western Ghats and maintained at JNTBGRI to conduct the pharmacological studies. Extraction procedures were carried to prepare the drugs of different doses for the study. Acute oral toxicity studies in mice and antiinflammatory studies in rats, were done as pharmacological analysis. The preliminary phytochemical investigation, tubers of Chlorophytum laxum has shown the presence of secondary metabolites like carbohydrates, phenols, alkaloids, proteins, steroids, tannin, saponins and glycosides that may be responsible for its medicinal properties. The content of total phenols in the ethanolic extract of Chlorophytum laxum expressed as gallic acid equivalents per gram of dry extract is 5.12 mg GA/g of extract. Toxicity studies of tuber extract were investigated in Swiss albino mice for 14 days by the administration of 4 doses 5, 50, 300 and 2000 mg/kg body weight and no symptoms of toxicity were seen in the animals even up to the highest dose. In detailed pharmacological studies, antiinflammatory potential of tuber extract was investigated in vivo by carrageenan induced paw oedema and formalin induced paw oedema and in vitro by HRBC membrane stabilization assay. The extract were administered at doses of 50, 150 and 450 mg/kg body weight orally in adult wistar rats and the maximum percentage inhibition of paw oedema in the right hind limb was shown by ECL 450 mg/kg in both the methods. ECL at higher concentration protect significantly the hypotonicity induced haemolysis of HRBC by in vitro antiinflammatory analysis. The antioxidant effect of ethanolic extract of C. laxum showed IC50 of 36.62 μg/mL in hydroxyl radical scavenging, 68.91 μg/mL in nitric oxide radical scavenging and 135.67 μg/mL in antilipid peroxidation assay. Total antioxidant capacity of ethanolic extract of tubers of C. laxum was found to be 90.04 μg AAE/g of dry extract. The antioxidant potential of C. laxum was compared with a standard and the results obtained gives the significant effect and almost equal effect. The results of current study will help to develop a monograph of the drug for reference. These results substantiate the traditional claim of the plant for its medicinal use.
