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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Characteization and evaluation of nutmeg (Myristica fragrans Houtt.) accessions(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 2016) Vikram, H C; KAU; Mini Raj, NNutmeg (Myristica fragrans Houtt.) is an introduced crop to India. There exists tremendous variability in the nutmeg population in Kerala, which is the major nutmeg growing state in the country. Assessment of the existing variability is a prerequisite for taking up successful crop improvement programmes, which is very much limited in this tree spice. In this context, the present study entitled “Characterization and evaluation of nutmeg (Myristica fragrans Houtt.) accessions” was taken up exclusively with the specific objectives to characterize nutmeg accessions based on morphological, biochemical and molecular parameters so as to scale the variability in a multidimensional way. Select fifty nutmeg accessions from a core germplasm collected and maintained in a private plantation in the Chalakudy river basin, belonging to age of fifteen years, formed the material for the study. Among the select fifty accessions, forty two were females, four monoecious and four males. In the morphological characterization, 51 qualitative and 38 quantitative characters were recorded from two trees per accession. Biochemical characterization was done in the select seventeen distinct accessions. GC-MS profiling was done in kernel and mace oils. Biochemical constituents of fresh pericarp were estimated. Isozyme profiling was done for peroxidase and polyphenol oxidase enzymes. The molecular characterization was attempted with 21 RAPD and 12 ISSR primers after screening. A key for identification of an elite nutmeg tree was developed. A descriptor for nutmeg with a set of 51 qualitative and 38 quantitative parameters and descriptor states for each of these characters was developed as the first step. This is the first study of its kind to develop a minimal descriptor for nutmeg. The descriptor developed from the present study was simultaneously utilised for morphological characterization and evaluation of the accessions. Wide variability was noticed among the accessions for 47 out of 51 qualitative characters. Four characters viz., leaf margin, fruit pubescence, grooves on nut and nature of fruit dehiscence were noted as non variable characters and hence, these were not included for further analysis. Based on the qualitative characters, accessions were classified into 11 clusters at 66 per cent similarity level. Accessions differed significantly for all the quantitative characters except shelling percentage. Performance evaluation of the accessions brought out the superiority of accession 8, 9 and 22 for yield. The accessions showed high GCV, PCV, h2 and genetic gain for most of the characters. Number of fruits per tree, fruit set percentage, number of fruits per m2, fresh and dry weight of mace, mace volume as well ratio of nut to mace exhibited high genetic gain. Hence, selection programme based on these characters will be very effective in improving the base populations. Based on Mahalanobis D2 analysis, accessions were grouped into 10 clusters. Wide range of variation was observed in contents of volatile oil, oleoresin and fixed oil of kernel and mace. Based on these constituents accessions were grouped into 26 clusters, which indicated their distinct quality. Based on the results of the morphological characterization, seventeen distinct accessions were selected for further biochemical and molecular analysis. GC-MS analysis of kernel and mace oils exhibited 20 and 24 constituents respectively. Volatile oil composition exhibited wide variability for the major constituents viz., myristicin, elemicin, safrole and sabinene apart from the presence of some unique compounds. Grouping of the accessions was done based on the per cent content of these important compounds. Two accessions recorded high contents of both myristicin and elemicin whereas another two accessions were in the complimentary; belonging to low myristicin group. High sabinene combined with low myristicin was the intrinsic quality attribute of one of the accessions. Change, as well as addition/deletion of specific constituents was also noticed in the volatile oils after storage for one year. Accessions exhibited wide range of variation in the biochemical constituents of pericarp, a valuable information for the value addition of pericarp. Total phenol and tannins exhibited high variation. The accessions were ranked based on the content of biochemical constituents. Isozyme profiling using peroxidase enzyme produced four bands and that based on polyphenol oxidase exhibited three bands. Molecular markers could assess the variability among the accessions. The selected 21 RAPD primers produced a total of 164 amplicons of which 63.21 per cent were polymorphic. The 12 ISSR primers selected produced a total of 87 amplicons of which 69.44 per cent were polymorphic. Few unique bands were detected for specific characters. Inter cluster association of each of the qualitative clusters with other clustering patterns was worked out. The results indicated the differences as well as similarities of the qualitative clusters with other clustering patterns. Finally, key quantitative characters were identified based on their direct and indirect effect on yield as also economic importance. The statistical key thus developed using 13 key quantitative characters will serve as a preliminary tool for identification of an elite nutmeg tree.ThesisItem Open Access Efficiency of vermiconversion and decomposition of farm residues on soil health, yield and quality of banana (Musa spp.)(Department of Science and Agricultural Chemistry, College of Horticulture,Vellanikkara, 2016) Mayadevi, M R; KAU; SushamaAn investigation entitled “Efficiency of vermiconversion and decomposition of farm residues on soil health, yield and quality of banana (Musa spp.)” was undertaken at College of Horticulture and Banana Research Station, Kannara during 2013-2015. The objectives of the study were to compare the efficiency of native and exotic earthworms on vermiconversion of farm residues and the effects of different modes of vermicomposting on soil health, yield and quality of banana and to evaluate the in situ decomposition of banana crop residues An experiment with seven treatments and three replications was laid out in randomized block design to assess the composting efficiency of native and exotic earthworms. Seven treatments included absolute control as soil (S), ex-situ compost in silpaulin vermibed without earthworms (Ex-C), ex-situ vermicompost in silpaulin vermibed using Perionyx excavatus (Ex-P), ex-situ vermicompost in silpaulin vermibed using Eisenia foetida (Ex-E), in-situ vermicompost in banana planting pits using Perionyx excavatus (In-P), in-situ vermicompost in banana planting pits using Eisenia foetida (In-E), in-situ compost in banana planting pits without earthworms (In-C). In the next study, the prepared composts were tested along with recommended doses of fertilizers and FYM to assess their effects on growth, yield and crop quality of banana var. Nendran in RBD with seven treatments replicated thrice. The treatments were absolute control (S), 300:115:450g N:P2O5:K2O plant-1 + FYM at the rate of 20 kg plant-1 (POP) , exsitu vermicompost in silpaulin vermibeds with Perionyx excavatus at the rate of 20 kg plant -1 (Ex–P), ex-situ vermicompost in silpaulin vermibeds with Eisenia foetida at the rate of 20 kg plant -1 (Ex–E) , in-situ vermicompost in crop pits with Perionyx excavatus at the rate of 20 kg plant -1 (In-P), in-situ vermicompost in crop pits with Eisenia foetida at the rate of 20 kg plant -1 (In-E), in -situ compost at the rate of 20 kg plant -1 (In-C). After the harvest of the crop, the entire residues from each plant was incorporated in the respective crop pits and the insitu degradation of the banana residues was monitored. The salient findings are summarized as follows In the first trial, the composting efficiency of native and exotic earthworms under different modes of composting was compared. Chemical properties of compost like pH, electrical conductivity, and content of primary nutrients were significantly affected by modes of composting. Exotic earthworms like Eisenia foetida produced higher auxin content in the compost whereas Perionyx excavatus registered higher dehydrogenase activity under ex-situ mode of composting. The total microbial load was recorded in the compost prepared without earthworms under both modes of composting. Compost yield was more in ex-situ composting methods and Eisenia foetida was more efficient in composting as compared to Perionyx excavatus. Humic acid characterization by Fourier Transform Infra-Red spectroscopy revealed a similar spectra from different treatments and were found to be characterized by aromatic rings and triple bonded skeleton. Presence of relatively higher quantity of polysaccharide like substances was observed in insitu mode of composting. The spectra of humic acids for ex-situ composts prepared using Perionyx excavatus revealed a nitrate band of characteristic shape appearing prominently. Perionyx excavatus produced humic acids with high aromaticity both under ex-situ and in-situ mode of composting. Scanning electron microscopy revealed neo-formed CaCO3 nodules embedded in humic acids in the ex-situ vermicompost produced by Eisenia foetida. Transmission Electron Microscopy of humic acids indicated a globular structure for vermicompost prepared by Perionyx excavatus and a lamellar structure with voids for vermicompost prepared using Eisenia foetida. On field experimentation with different treatments using Nendran banana as the test crop, the highest yield was obtained for combined application of fertilizers and farm yard manure as per POP. Number of fingers per hand and number of functional leaves were found to influence the yield, irrespective of treatments. The number of days to bunching and harvest was significantly reduced for banana under in-situ composting. Higher content of total, reducing and non-reducing sugars were observed for in-situ composting treatments. On the contrary, higher titratable acidity was recorded for the control treatment. Both vitamin C content and sensory parameters were complimentary for POP treatment. Increased absorption and translocation of nutrients such as N, P, K and Mg in fruits were observed under organic nutrition using Perionyx excavatus. For the next study, the residues of banana were added to the respective pits for monitoring the carbon and nitrogen mineralization. The carbon mineralization with respect to time was fitted to single pool exponential model to derive the rate of mineralization and half-life. It revealed crop residues pre-treated with vermicompost using exotic earthworms had a higher rate of C cumulative mineralization. Nitrogen addition by both exotic and native species of earthworms were almost similar under in-situ conditions. The banana residue decomposition by Eisenia foetida was about 3.5 times higher than that of Perionyx excavatus. Calcium content of Perionyx excavatus pre-treated residues added the maximum calcium to the soil.ThesisItem Open Access Silicon availability of tropical soils with respect to rice nutrition(Department of Soil Science and Agricultrural Chemistry, College of Horticulture,Vellanikkara, 2016) Arya Lekshmi, v; KAU; Jayasree Sankar, sSilicon (Si) is the second most abundant element in soil. The amount of silicon in soil depends on parent material, soil type, pedogenic process and landscape. In soil solution, Si is present as monosilicic acid which is the only form that the plant can absorb from soil. The productivity of rice is comparatively low in soils of Kerala. As a ̳Si – accumulator‘, rice can benefit from Si nutrition. The application of Si can enhance growth and yield of rice. With this background, studies were conducted to categorize major rice growing soils of Kerala according to plant available silicon and to evaluate the efficacy of different sources of silicon including rice straw in wetland rice. The release of silicon from different soils added with various silicon sources under different water regimes was also monitored. Soil samples were collected from five different locations representing major rice growing regions of Kerala viz., Kuttanad, Kole land, Pokkali, sandy and lateritic to categorize them according to plant available silicon. The available Si ranged from 7.70 mg kg -1 (sandy soil) to 34.91 mg kg -1 (Kole land soil) in the order Kole land > Pokkali > lateritic > Kuttanad > sandy soil. All the soils under study were categorized as low in available Si. The available Si had positive correlation with organic carbon, available N, Ca, Mg, Fe, Mn, Zn, exchangeable K, Ca, Mg and CEC and negative correlation with available boron, AEC and silica-sesquioxide ratio. These soils were subjected to fractionation of silicon. The major fractions of Si were mobile, adsorbed, organic, occluded, amorphous and residual Si. The percentage distribution of fractions of Si in these soils were in the order; residual Si > amorphous Si > occluded Si > organic Si > mobile Si > adsorbed Si. Quantity – intensity relationship of five major rice growing soils at two temperatures viz. 25 0 C and 40 0 C were studied. The highest buffer power was indicated by Kuttanad soil followed by Pokkali and sandy soils at 25 0 C. It clearly indicated that these soils have a higher power to retain Si on solid phase and replenish its concentration in soil solution as and when it is depleted through plant uptake or leaching. The equilibrium Si concentration and the amount of Si adsorbed by each soil were used to test the fitness of data to the adsorption isotherms viz., Langmuir, Freundlich and Temkin. The data obtained from the adsorption experiments fitted into Freundlich and Temkin equations, but not to Langmuir equation at 25 0 C. At 40 0 C no adsorption equations were obtained for any soil.An incubation study was conducted to know the extent of release of Si on addition of different sources of silicon such as rice husk ash, biodecomposed rice husk, calcium silicate and sodium silicate in five rice growing soils under submerged water regime (SWR) and field capacity water regime (FCWR). Addition of Si significantly increased the release of available Si in all soils except Kole land soil after a month. Kole land soil showed higher release of available Si after two months. The highest release of available Si was at SWR in case of Kole land and Kuttanad soil, where as Pokkali, sandy and lateritic soils showed more release of available Si at FCWR. Irrespective of soils, treatment with sodium silicate showed higher release of available Si. Total Si showed a decreasing trend over the period of incubation for three months in all the soils. A field experiment was conducted at Agronomic Research Station, Chalakudy to evaluate the efficacy of different sources of silicon including rice straw in wetland rice. Rice husk ash, biodecomposed rice husk, calcium silicate and sodium silicate were used as source of Si along with fertilizers as per package of practice recommendation (NPK alone). The maximum number of panicles per hill, number of spikelets per panicle, thousand grain weights and minimum number of unfilled grains per panicle were recorded in treatment with calcium silicate application. The maximum grain yield of 6.90 t ha -1 was recorded in treatment T 5 (T 2 + Calcium silicate) and significantly superior (fig.54) over all other treatments. This increase in yield may be due to the effect of application of Si on soil fertility, nutrient uptake, and plant growth. The direct effect of Si fertilization on increased number panicle per hill, number of spikelets per panicle, and thousand grain weight and decreased number of unfilled grains per panicle might be the reason for increased grain and straw yield in treatment with calcium silicate. The treatment with POP + sodium silicate showed the highest uptake of Si by grain and straw of rice. The sources of Si had no residual effect on grain and straw yield of succeeding rice crop. In general, sandy soil low in available Si had a high response to applied Si in achieving higher grain yield.ThesisItem Open Access Integrated management of foliar fungal disease of culinary melon (Cucumis meloL. var. acidulus Naudin)(Department of Plant Pathology, College of Agriculture, Vellayani, 2016) Narmadhavathy, S; KAU; Kamala NayarThe project entitled “Integrated management of foliar fungal disease of culinary melon (Cucumis melo L. var. acidulus Naudin)” was undertaken with the objective of making a comparative evaluation of the efficacy of foliar application of fertilizers, micronutrients, bio-control agents and newer fungicide for the management of Colletotrichum leaf spot (Colletotrichum sp.) disease of culinary melon. Surveys conducted during September 2013 to December 2013, in ten culinary melon fields located at Instructional Farm (IF), College of Agriculture (CoA), Vellayani as well as in farmers’ fields near, CoA, Vellayani, in order to assess the prevalence of major diseases such as Colletotrichum leaf spot and downy mildew disease affecting the crop. Highest disease incidence (DI) and percentage disease index (PDI) of Colletotrichum leaf spot were observed, 75 days after sowing, at Chavadinada (70.00 per cent and 64.44 per cent respectively). Incidence and index of downy mildew disease were recorded in four out of the ten locations surveyed (Palapoor, Papanchani, Kalliyoor and Punjakari). Maximum disease incidence and percentage disease index of downy mildew disease (36 per cent and 33.33 per cent respectively) were observed at Papanchani. The most virulent isolate of anthracnose leaf spot pathogen (IF, Vellayani isolate), obtained during the survey was identified as Colletotrichum fructicola by molecular characterization. The treatment NPK 19:19:19 (0.5 per cent) combined with the fungicide mancozeb (0.4 per cent) and adjuvant was most effective in inhibiting the mycelia growth of the pathogen C. fructicola, in vitro, (100 per cent) over control as well as in suppressing artificially induced anthracnose disease and improving the growth parameters of the plants, in the two greenhouse experiments conducted at the CoA, Vellayani during March to June 2014 and August to October, 2014. Results of two field trials conducted at CoA, Vellayani, during January to March, 2015 and April to June, 2015 for testing four most effective treatments screened from the greenhouse experiments, indicated that NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15 ml/l) + adjuvant (DI 40.00 and PDI 13.05 respectively) and NPK 19:19:19 (0.5 per cent) + mancozeb (0.4 per cent) + adjuvant (DI 40.00 and PDI 13.47 respectively) were most effective in managing the disease and also increasing total yield of plants, when compared to the remaining treatments. Trials were conducted in farmers’ fields at three locations (Venganoor, Vavamoola and Venjaramoodu) for confirming the efficacy of the two most effective treatments screened from the field trials conducted at CoA, Vellayani and pooled analysis of the results indicated that the lowest PDI (12.22) and DI (28.50) were obtained in plants treated with NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15ml/l) + adjuvant, which was significantly superior to the other treatments. Results of the microbial studies indicated that there was decline in fungal flora of the plants treated with foliar fertilizer NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15 ml/l) + adjuvant, days after application of treatments whereas bacterial population was higher in plants applied with the same treatment when compared to the application of combination of foliar fertilizer NPK 19:19:19 (0.5 per cent) + mancozeb (0.4 per cent) + adjuvant. There was indication of higher induction of systemic resistance in plants treated with NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15 ml/l) + adjuvant due to the higher activity of defense related enzymes, such as phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), β-1,3glucanase, super oxide dismutase (SOD) and the compound phenol, all of which, reached maximum level on the 15th day after treatment. Leaf samples obtained from plants treated with foliar fertilizer NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15 ml/l) + adjuvant indicated highest nutrient use efficiency in all three locations of the confirmation trials while highest pigment status due to this treatment was observed in the trial conducted at Venganoor. Relative water content was generally high in leaf samples collected from all plants irrespective of the treatments, although it was comparatively low, in leaf samples obtained from plants of absolute control plot. Epicuticular wax content was slightly lower in the plants treated with combination of the foliar fertilizer NPK 19:19:19 (0.5 per cent) and fungicides, either azoxystrobin (0.15 ml/l) or mancozeb (0.4 per cent) + adjuvant. Stomatal frequency on the upper and lower surfaces of leaves was not much affected by application of foliar fertilizer NPK 19:19:19 (0.5 per cent) combined with the fungicides. B:C estimated ratio revealed that the highest returns were obtained from the plants treated with foliar spray of NPK 19:19:19 (0.5 per cent) + azoxystrobin (0.15 ml/l) + adjuvant, in all three locations of the farmers’ field trials. This study presents the first report of the pathogen Colletotrichum fructicola causing anthracnose leaf spot disease of culinary melon in India. In field conditions, combination of the foliar fertilizer NPK 19:19:19 (0.5%) and azoxystrobin (0.15 ml/l) along with adjuvant applied twice at 15 days’ interval was most effective in controlling anthracnose leaf spot disease of culinary melon and also increasing the yield of the crop.ThesisItem Open Access Herbicide mixtures for weed management(Department of Agronomy,College of Agriculture, Vellayani, 2016) Sheeja, K Raj; KAU; Elizabeth, K SyriacThe experiment entitled "Herbicide mixtures for weed management in direct seeded puddled rice Oryza saliva L." was carried out at College of Agriculture, Vellayani, during the period from 2013 - 2016, to assess the bioefficacy of two post emergence herbicide mixtures viz., bispyribac sodium + metamifop and penoxsulam + cyhalofop butyl in direct seeded rice; to study the residual effect in soil; to investigate the impact on microbial and earth worm population, soil enzyme activity and weed seed bank and also to assess the in vitro sensitivity to soil borne pathogen, Rhizoctonia solani, bio control agents and bio fertilizer organisms. Field experiment was undertaken during first and second crop seasons of 2014-15 at Nemom block at farmer's field. The experiment was laid out in RED with 12 treatments and three replications. Bispyribac sodium + metamifop @ 60, 70, 80 and 90 g ha"*,penoxsulam + cyhalofop butyl @ 120, 125, 130 and 135 g ha"*, bispyribac sodium @ 25 g ha"', penoxsulam @ 22.5 g ha"', hand weeding twice and weedy check constituted the treatments. The higher three tested doses of penoxsulam + cyhalofop butyl v/z.,125, 130 and 135 g ha ' and the highest tested dose of bispyribac sodium + metamifop viz., 90 g ha"' were better than other weed management treatments in improving the growth and yield attributes of rice. Pooled analysis indicated the superiority of penoxsulam + cyhalofop butyl @ 135 g ha"' which recorded the highest grain yield (8463 kg ha ' ) and it was statistically on par with its lower doses (130 and 125 g ha*'). Straw yield was not significantly influenced by the weed control treatments. Penoxsulam + c^lofop butyl @ 135 g ha"' recorded the highest net returns (93744 ha *) and B: C ratio (2.43), which was on par with its lower dose (130 g ha"'). Penoxsulam + cyhalofop butyl @ 135 g ha"' was more effective in reducing the total density of weeds. With regard to the control of sedges and BLW, penoxsulam + cyhalofop butyl was more effective, but with respect to grasses, both the herbicide mixtures were more or less similar in effectiveness. Penoxsulam + cyhalofop butyl @ 135 and 136 registered higher weed control efficiency and lower weed index than other treatments. Uncontrolled weed growth caused a yield reduction of 50.38 per cent. Herbicide treatments significantly reduced the nutrient uptake by weeds and enhanced the nutrient uptake by crop over weedy check. The higher doses of penoxsulam + cyhalofop butyl (125,130 and 135 g ha"') and bispyribac sodium + metamifop (70, 80 and 90 g ha*') were more effective in maintaining higher nutrient content in soil. Dynamics of soil microbial population, earthworm population, soil enzyme status and organic carbon content of soil consequent to the application of herbicide mixtures revealed that, both penoxsulam + cyhalofop butyl and bispyribac sodium + metamifop at their tested doses did not have any inhibitory effect. Results of screening trial revealed that maize was the most sensitive indicator plant for both the herbicide mixtures. Dry weight and fresh weight of maize shoot were adjudged as the best parameters for assessing the residual effect of bispyribac sodium + metamifop and penoxsulam + c>^Iofop butyl in soil respectively. Pot culture bioassay studies revealed that the tested herbicide mixtures and their doses did not have any residual effect. In vitro sensitivity studies of bispyribac sodium + metamifop and penoxsulam + cyhalofop butyl to Rhizoctonia solani pointed out that both the herbicide mixtures inhibit the growth of Rhizoctonia solani. Studies on the in vitro effect of herbicide mixtures to Trichoderma viride indicated that bispyribac sodium + metamifop was safe to the fungus, whereas penoxsulam + cyhalofop butyl was moderately toxic. Compatibility studies revealed that both the herbicide mixtures were highly compatible with Psetidomonas fluorescenSy Azospirillum lipoferum and Azotobacter chroococciim. Weed seed bank assay results indicated the effectiveness of the higher doses of penoxsulam + cylialofop butyl (135, 130 and 125 g ha'') in depleting the seed bank compared to other treatments. The present study revealed the superiority of heibicide mixtures over individual herbicides in the management of weeds in wet seeded rice. Application of penoxsulam + cyhalofop butyl @ 135 or 130 g ha*', at 15 DAS could be adjudged as the most economic and ecofriendly weed management practice for wet seeded rice as evidenced by high weed control efficiency, net returns and B: C ratio; environmental safety, high inhibitory effect to Rhizoctonia solani and good compatibility with bio control agents and bio fertilizer organisms.ThesisItem Open Access Indicators of sustainable agricultural development: a multi-variable analysis among self help groups of "kudumbashree mission" in Thiruvananthapuram district.(Department of Agricultural Extension, College of Agriculture, vellayani, 2016) Chinchu, V S; KAU; Padmanabhan, V BThe study entitled “Indicators of sustainable agricultural development: A multi-variate analysis among self-help groups of “Kudumbashree Mission” in Thiruvananthapuram district” was undertaken with the specific objective of critically analyzing the extent of attainment of the three pillars of sustainable development, namely, economic development, social develop¬ment and environmental protection by the agricultural activities of the self-help groups under Kudumbashree Mission in the study area and to identify the constraints and formulate a strategy for increasing the effectiveness of the programme. Exploratory or Formulative Research Design was employed for the study. The sustainability was studied among 40 agriculturally active SHGs. Profile characteristics of 200 women farmers were also studied. The study was undertaken in the Thiruvananthapuram district of Kerala state. Out of the 12 development blocks in the district, five blocks were randomly selected, namely, Parassala, Perumkadavila, Nemom, Nedumangad and Vellanad. From each of the blocks, one agriculturally active Community Development Society (CDS) was purposively selected, namely, Kunnathukal, Karode, Malayinkeezhu, Karakulam and Kattakkada. There were two categories of respondents for the study. Two hundred number of SHG-based farm women formed the first category of respondents and 40 number of agriculturally active SHGs formed the second category of respondents. From each of the Community Development Society, 40 number of agriculturally active and SHG-based farm women were randomly selected. These added upto 200 individual respondents. Eight agriculturally active SHGs were also randomly selected from each of the five Community Development Societies and these added upto 40 SHGs. Fourteen variables were used to study the individual SHG members. ANOVA was done where ever possible to find out if there existed any difference between each of the five sample CDSs for any particular variable. Seventeen independent variables related to SHGs were also studied. An index namely, Sustainable Agricultural Development Index (SADI) was developed as part of the study for exclusively measuring the sustainability of group farming by the women groups. The index covered eighteen variables related to the economic, social and environmental aspects of group farming. Principal Component Analysis (PCA) was also done using the software SAS 9.3 to find out the relative importance of each of the indicators and sub-indicators towards the sustainability of group farming. The strengths, weaknesses, potentials and challenges of SHG-based farming were also found out using SWPC analysis. The constraints faced by both individual SHG members as well as the groups as such were found out and ranked. Finally the suggestions for improvement of SHG-based farming and suggested lines of future research were also spelt out based on the results of the study and the on-field experiences of the researcher. The study could find out that majority of the sample SHG-based women were of middle aged and were having nuclear families and secondary education. They were engaged in some income generating activities other than agriculture. They had good experience in both SHG based activities and in agricultural activities. Krishibhavan was found to be the most prominent information source for those women. The SHG-based women were found to possess a good level of self confidence, innovativeness, scientific orientation, achievement motivation, economic motivation and risk orientation. It was observed that as they grew older, both SHG experience and farming experience increased but their level of innovativeness went on decreasing. Regarding the SHGs, the study revealed that their mean size was 4.33 and had a mixed population of BPL members, APL members, general category members, OBC members and SC/ST members. Invariably all the sample SHGs were cultivating banana and 70 per cent of the groups were cultivating two or more crops. Also 70 per cent of the groups had availed loans with a mean amount of Rs. 1,40,714. As high as 95 per cent of the groups used to hire external labour especially for initial land preparation. The mean area cultivated by a sample SHG was found to be 190.53 cents. On economic front, all the groups were found to have a high degree of economic motivation. The BC ratio of majority of the groups was found to be medium. Eighty per cent of the groups showed an increase in their income over the previous year and also could generate a good number of day’s employment in agricultural sector. As the area cultivated had increased, addition in the income and employment generation were also found to increase. The study elucidated area cultivated (AC) and benefit-cost ratio (BCR) as the most important sub-indicators of economic development. On social front, the study revealed that majority of the groups were possessing a high transparency, equity, leadership, cohesiveness, accountability, team spirit and co-operation with ‘team spirit’ followed by ‘equity’ as the most important sub-indicators of social sustainability of Kudumbashree SHGs. Regarding environmental protection, ‘adoption of soil conservation measures’ and ‘avoidance of chemical fertilizers’ were found to be the most important sub-indicators affecting environmental sustainability. A quarter of the Kudumbashree groups were found to follow organic farming, around 20 per cent followed strict chemical-based modern agriculture and the rest followed a middle path between organic farming and modern agriculture. The Sustainable Agricultural Development Index (SADI) for the studied sample was found to be 0.69. It could be concluded that though the agriculturally active Kudumbashree SHGs in the study area had reasonable economic sustainability and social sustainability, their performance in environmental protection was a bit lower and only if they strengthen this weak link, they can become truly sustainable. Crop loss due to pests, diseases, wild animals and other climatic factors was the foremost constraint expressed by the respondents of the study. The study recommends establishment of ‘Woman Farmer Producer Companies’ (WFPC) at taluk level under the direct control of the respective District Missions or adoption of ‘Anand Pattern Co-operative Method’ as two viable strategies for improving the sustainability of agricultural activities performed under Kudumbashree mission.ThesisItem Open Access Strain evaluation and production technology of shittake mushroom ( Lentinula edodes ( Berk. ) pegler)(Department of Plant Pathology, College of Agriculture, Vellayani, 2016) Deepa Rani, C V; KAU; Lulu DasThe present investigation on "Strain evaluation and production technology of Shiitake mushroom (Lentinula edodes (Berk.) Pegler' was conducted at Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram during the period 2012-2015. The aim of the experiment was to exploit various strains of Lentinula spp. for novel production technology and their phylogeny analysis through physiological and molecular studies. Surveys were collected during pre and post monsoon periods of May to December from different parts of Thiruvananthapuram, Kollam, Wayanad, Idukki, Pathanamthitta, Kannur and Kasargode districts. Six isolates of sp. (VLYN- 1 to VLYN-13) obtained during the survey were identified and compared with procured reference strains of Lentinula edodes (LE-1 to LE-5 from GB Pant University of Agricultural and Technology, Pantnagar, Uttarakhand) and LE-6 strain (Maharana Pratap University of Agriculture and Technology, Udaipur) . Morphologically the native isolates of Lentinus spp. had concave, funnel and convex pileus with varying colors and were leathery in nature.L. edodes strains in contrast had convex pileus with chocolate brown and golden yellow sporocarps which were fleshy and edible. Phylogenetic analysis of all six strains of L. edodes using RAPD markers confirmed the variability between the strains. Maximum similarity coefficient of 74.10 per cent was observed between LE-2 and LE-6 strains while LE-2 and LE-4 strains showed a minimum similarity coefficient of 35.70 per cent. Further studies by ITS sequencing showed that all the L. edodes strains tested in the study showed 99- 100 per cent similarity with the known sequences off L. edodes available in NCBI database while that of native isolates showed 99- 100 per cent similarity to Lentinus tuber-regium and Lentinus connatus thus confirming the variability between Lentinus and Lentinula sp. All the six strains of L. edodes, showed maximum mycelial growth in malt extract peptone dextrose agar in solid and oat meal broth in liquid medium. L. edodes strains preferred temperature of 20 °C with an acidic pH of 6. Dark and ambient light conditions favored maximum mycelial growth and biomass production for L. edodes culture. Although a minimum period of 16.33 days was required for full mycelial run in maize grains but due to comparatively less contamination rate in paddy grains which took 18.33 days for completion of mycelial run were selected as best substrate for further studies. Different substrates were evaluated for the development of a cultivation package for shiitake mushroom. Results showed that LE-1 strain took minimum of 71.00 days for initiation of sporocarp in sawdust supplemented with 20 per cent wheat bran. Hard wood sawdust especially of teakwood was used in the study. The substrate based on paddy straw and banana pseudo stem were not found effective for pinhead initiation and thus failed to produce sporocarps. LE-1 produced maximum sporocarp (11.33) in sawdust + 20 per cent wheat bran which was followed by LE-3 (10.63) in sawdust + 20 per cent rice bran. Maximum yield of 290.66 g/ 500 g substrate was obtained in sawdust + 20 per cent wheat bran by LE-6 strain. Maximum biological efficiency of 58.13 per cent was also recorded in LE-6 in sawdust supplemented with 20 per cent wheat bran substrate. Substrates like paddy straw and sawdust amended with 20 per cent wheat bran substrates were evaluated for the development of native isolates of Lentinus tuberregium and Lentinus connatus . Results showed that maximum biological efficiency of 58.00 per cent was obtained by Lentinus tuber-regium whereas 36.60per cent biological efficiency by Lentinus connatus in sawdust amended with 20 per cent wheat bran substrate. Nutrient analysis of all the six strains showed that carbohydrate content ranged between 35.29 per cent to 40.23 per cent, protein 18.33 per cent to 21.66 per cent, crude fibre 22.33 per cent to 27.33 per cent, Vitamin- C 2.53 per cent to 3.50 per cent, ash 2.70 per cent to 4.40 per cent and lipid 2.46 per cent to 3.60 per cent. Mineral content of L. edodes included Ca (11.00 mg to 19.00 mg/ 100 g), Mg (0.46 to 1.10 mg/ 100 g), Fe (1.36 mg to 1.80 mg/ 100 g), Mn (1.53 mg to 2.63 mg), P (1.65 mg to 2.87 mg), K (16.33 mg to 25.20 mg), Na (13.00 mg to 23.66 mg) and Zn (19.66 mg to 28.33 mg/ 100 g). Sensory evaluation of mushroom products made from L. edodes was carried out by a panel of judges for various characters of which mushroom masala scored maximum for texture, taste, flavor and overall acceptability when compared to other recipes like mushroom cutlet, scramble, soup, baji and biscuit. As part of the study, paddy grain was found to be the most suitable substrate for spawn production of L. edodes and teakwood sawdust amended with 20 per cent wheat bran was the most efficient bed substrate. LE-6 strain was superior in terms of yield and biological efficiency. Therefore findings of the above investigation recommends the adoption of a suitable cultivation package for shiitake mushroom by using low cost substrates (hardwood sawdust) available in Kerala in plains and hilly regions.ThesisItem Open Access Development of small interfering RNA (siRNA) mediated resistance in banana against banana bract mosaic virus(Department of Plant Biotechnology, College of Agriculture, Vellayani, 2016) Lekshmi, R S; KAU; Soni, K BThe present study entitled “Development of small interfering RNA (siRNA) mediated resistance in banana against Banana bract mosaic virus (BBrMV)” was carried out during 2012-2016 in the Department of Plant Biotechnology, College of Agriculture, Vellayani. The study was carried out with an objective to develop siRNA mediated technology for the development of banana plants resistant to Banana Bract Mosaic Virus (BBrMV). The study was conducted in banana cv. Nendran. A protocol for somatic embryogenesis in banana cv. Nendran was standardized by using immature male flowers as explants. Pale white friable callus with rich cytoplasm was initiated in Murashige and Skooge (MS) medium supplemented with BA (0.1 – 0.5 mgL-1) and picloram (0.5 – 2 mgL-1) incubated in dark with a maximum explant response of 30 per cent. For embryogenesis, the developed embryogenic calli were transferred to semisolid MS medium supplemented with BA 2 mgL-1 and IAA 0.5 mgL-1 which resulted in a maximum of 10 per cent embryogenesis. The glassy elongated monocot embryos were germinated in half strength semisolid MS medium (0.3 per cent Gelrite) supplemented with BA 2 mg L-1 and IAA 0.5 mg L-1 and incubated in dark. A maximum germination rate of 80 per cent was obtained in this medium. The germinated embryos were transferred to MS medium with BA 2 mg L-1 and NAA 1 mg L-1 resulted in 100 per cent Plant regeneration. The plantlets were transferred to coirpith compost in pot trays in mist chamber for one month for hardening and then transferred to polybags with soil and cowdung (1:1) mixture. To develop siRNA technology to silence the replicase gene of BBrMV, an intron hairpin RNA (ihpRNA) construct was developed. For this a partial mRNA sequence of replicase gene was isolated from BBrMV banana plants. Gene specific primers designed based on the whole genome sequence information retrieved from the GenBank, NCBI. Total RNA from infected banana leaves was isolated and cDNA was prepared using RT-PCR. The partial gene fragment isolated was sequenced and analysed using the bioinformatics tool BLAST. The sequence was subjected to miRNA target prediction and restriction mapping to select suitable region for the construct and further processing. Based on this information a fragment of 400 bp towards the 5’ end was amplified by designing a set of primers with anchored restriction sites. The primers anchored with BamHI and PacI sites were used for the amplification of sense strand and primers anchored with KpnI and SpeI sites were used for antisense strand amplification. The sense and antisense fragments amplified were cloned to pTZ57R/T cloning vector. In the next step the inserts were released from pTZ57R/T using the corresponding restriction enzymes and were integrated in pSTARLING (primary vector), on either side of the cre intron which facilitated the formation of the hairpin (ihpRNA) construct. Presence of the inserts was confirmed by restriction digestion and electrophoresis. The ihpRNA construct in pSTARLING now contained ubiquitin promoter, ubiquitin intron, sense strand of replicase gene, cre intron, antisense strand of replicase and termination sequence in the order with the NotI restriction sites. This construct was released from pSTARLING and ligated to the digested NotI site in the lacZ gene of the binary vector pART27 containing antibiotic resistance marker nptII and spec. The binary vector was confirmed for the insert by transferring to DH5α and colony selection by blue-white screening. The binary vector with the insert isolated from the white colony, was transferred to Agrobacterium tumefaciens strain LBA 4404 via freeze-thaw method. Transformed colonies were picked up and confirmed the presence of the vector and the ihpRNA insert by PCR. Somatic embryos were transformed with LBA 4404 carrying the ihpRNA construct the ihpRNA construct and the transformed embryos were selected with antibiotic pressure (Kanamycin 100 mg L-1). Transformed embryos were subjected to regeneration. A maximum regeneration of 25 per cent was obtained after transformation. The regenerants were confirmed for the presence of ihpRNA construct using PCR with specific primers for sense-intron-antisense fragment, npt II and cre intron. The study was successful in developing a siRNA construct for resistance against BBrMV and obtaining transformed Nendran banana plantlets.ThesisItem Open Access Standardisation of two line heterosis breeding in rice ( Oryza sativa L.) for Kerala(Department of Plant Breeding and Genetics, College of Horticulture, Vellanikkara, 2016) Rajesh, T; KAU; Radhakrishnan, V VThe present investigation entitled “Standardisation of two line heterosis breeding in rice(Oryza sativa L.) for Kerala” was conducted in the Department of Plant Breeding and Genetics, College of Horticulture, Kerala Agricultural University during 2010-2013.The objectives of the study were to identify the promising TGMS lines of rice, for the production of two line hybrids suited to Kerala and to standardize the two line heterosis breeding programme. The experimental materials consisted of TGMS lines collected from Tamil Nadu Agricultural University and high yielding varieties of rice from different research stations of Kerala. The primary investigation was carried out at College of Horticulture, Vellanikkara to study the sterility/fertility behavior of TGMS lines .It was found that April and May seasons can be considered as the ideal period of exploiting 100 per cent sterility in rice for the hybrid seed production.Experiments at CRS Pampadumpara was the suitable location for TGMS line seed multiplication. The present study concluded that CST(Critical Sterility Temperature) ranged from 34.2 to 38.6 0 Cand CFT (Critical Fertility Temperature) ranged from 23.6 to 25.4 0 C. Natural pollination with suitable pollinator parents proved to be a viable method for the production of two line hybrid seeds.Aiswarya, Makom, Kanchana and Samyuktha were good general combiners whereas Kairali was a poor combiner . Per se performance and gca effects were not correlated for most of the characters evaluated. The two line hybrids TGMS 91S x Makom, TGMS82S x Aiswarya , TGMS 81S x Kairali, TGMS 81S x Kanchana, TGMS 91S x Kairali exhibited superior SCA effects for various characters in COH Vellanikkara . TGMS 82S x Samyuktha , TGMS 74S x Samyuktha, TGMS 81S x Aiswarya, TGMS 91S x Samyuktha, TGMS 74S x Kairali, TGMS 74S x Makom showed superior SCA effects for the characters studied at RARS Pattambi.Molecular analysis revealed that the number of alleles per locus ranged from 2 to 3. Polymorphic information content ranged from 0.291 (RM2) to 0.648 (RM132) with an average of 0.448. RM11 markers showed more number of alleles with PIC value more than 0.50 indicate the efficiency of this primer in detecting the most heterogenous accession. Cooking quality analysis of promising hybrids revealed intermediate amylose content and intermediate alkali spreading value for TGMS 91S x Makom , TGMS 91S x Kanchana, TGMS 81S x Makom and TGMS 81S x Aiswarya. TGMS 81S x MattaTriveni recorded highest grain and straw yield at COH Vellanikkara. TGMS 91S x Samyuktha was the best performer at RARS Pattambi.
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