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Kerala Agricultural University, Thrissur

The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively. Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively. On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs. In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc. During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU). Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.

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Subject: Plant Pathology × Author: Radhika, N S × End date: 2018 × Start date: 2018 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Integrated management of viral diseases of bittergourd (momordica charantia L.)
    (Department of Plant Pathology, College of Agriculture, Vellayani, 2018) Radhika, N S; KAU; Umamaheswaran, K
    The present research work entitled ‘Integrated management of viral diseases of bitter gourd (Momordica charantia L.) was carried out in the College of Agriculture, Vellayani during 2014-2017, with the objectives to study the occurrence and distribution of viruses in bitter gourd in Thiruvananthapuram, Idukki and Palakkad, immunomolecular characterization of the viruses, and screening of antiviral chemicals, antiviral principles of animal, plant and microbial origin for the management of the disease. In the suvey conducted at five locations in Thiruvanaanthapuram district, Pappanchani area recorded highest incidence of viral disease (60%) while highest Vulnerability Index (V.I) was recorded from Vellayani (56.00). In Idukki district, six major bitter gourd cultivating areas were surveyed among which Rajakumary area recorded the highest disease incidence (100%) and V.I (82.00). In Palakkad district, five locations were surveyed, among which panackatri and Thekkepotta recorded highest disease incidence of 88% and highest V.I (69.00). The major insects associated with the crop were whitefly (Bemisia tabaci (Genadius) with an incidence of 10-25%, aphids (Aphis gossypii glover) with an incidence of 10-40%, Jassids (Empoasca (Empoasca) motti Pruthi) with an incidence of 10-30% and mites with an incidence of 10-50%. Phyllody and little leaf symtoms (20% incidence) were also recorded in bittetgourd form Rajakumary and Rajakkad areas in Idukki. Flat limb and multiple proliferation of shoot tip were observed at many fields in Idukki. Symptoms associated with the disease include yellow mottle, mosaic,blistering, leaf curl and reduction in leaf size. Yellow mosaic and blistering is seen in severe infection finally leading to stunting of the plant, reduced flowering an fruiting and hairyness on stem. Mechanical transmission of the virus on Datura stramonium produced yellow lacal lesions indicating the presenceof Bean Golden mosaic virus (Begomo) in the infected leaf extract. This leaf extract also produced local lesions on othe indicator hosts like Chenopodium amaranticolor and Gomphrena globosa indicating the presence of Cucumber mosaic virus (CMV) or Potato virus Y (PVY). The viruses were transmitted by whiteflies (20%) and aphids (30%) from infected bittetgourd plants to healthy seedlings. Whiteflies (Bemisia tabaci Gennadius)) and aphids (Aphis gossypii Glover) are the vectors of the respective viruses Wedge grafting diseases scion on to 3-5 leaf stage healthy seedling of bittergourd produced symptoms of infection within ten days. KAU varieties Preethi and Priyanka were found to be susceptible to infection with preethi expressing a V.I of 70.80 and Priyanka expressing a V.I of 62.50 respectively. Ensyme linked immunosorbent assay (ELISA) and Dot immunobinding assay (DIBA) revealed the presence of three viruses belonging to Begomo, CMV and PVY group causing an mixed infection in bittergourd. The presence of all the three viruses were also confirmed in electron micrograph, Begomovirus as twin particles of size 18-20 X 30nm,CMVas single particles of 18nm and PVY as lonog flexuous rod of size 750nm. PCR amplification of coat protein gene (cp gene) of virus isolates from all the three districts yielded an amplicon of size approximately equal to 570 bp. Idukki and Palakkad isolates showed 94% identity to Tomato leaf Curl Virus isolate TNUDU BGI Coat Protein (AVI) gene while Trivandrum isolate showed 95% identity to Tomato leaf Curl Virus isolate TNPDU BG4 Coat Protein (AV1) gene . Phylogenetic tree constructed using multiple sequence alignment programme showed close relation between Begomo viruses identified in bittergourd from different districts. Studies on defense related enzymes such as peroxidase (PO), polyphenol oxidase (PPO) and phenyl alanine ammonialyase PAL) showed significant activity of PO and PPO in diseased plants than in healthy plants and the activity was on par in healthy and diseased for PAL. Protien profile of healthy and diseased at different days after virus inoculation through grafting indicated the production of novel proteins in diseased. There was no difference in the native profile of peroxidase in healthy and diseased at 15 days after virus inoculation. An additional isozyme band with a Rm value of 0.5 was observed in diseased at 45 days after virus inoculation. Management of the disease with antiviral chemicals and antiviral principles of plant, animal and microbial origin was undertaken as pot culture studies with pre and post inoculation of treatments. Twelve treatments with three replications each were laid out in completely randomized design for the evaluation. The treatments included Aspirin at two levels of 100 and 150 ppm, Salicylic acid (SA) at two levels of 100 and 150 ppm and Acibenzolar S methyl (ASM) at 50 and 75 ppm concentration, and two commercial formulations viz., Perfect and virus –Ex at 0.5 and 1.0 ml concentrations. The treatments were applied three times at 10 days interval. Pre application of thrice sprapying of Acibenzolar S methyl (ASM), 75 ppm concentration (V.I-35.00) at ten days interval was statistically significant over other treatments followed by ASM-50 ppm (V.I-41.33). Post application of antiviral chemicals also showed a statistically significant effect of three times spraying ASM-50 ppm(V.I-25.00) at ten days interval followed by spraying of Virus Ex 1ml L-1 (VThe best eight treatments with control was laid out as Randomised Block Design at the Instructional Farm, College of Agriculture, Vellayani during February to May 2017 as a field trial to study the effect of treatments on natural incidence of the viruses in the susceptible variety Preethi. The treatment, three sprays of ASM-50 ppm (V.I-28.33) at ten days interval ws on par with buttermilk (Three times dilution of curd) (V.I-39.16). Yield was also significantly high in ASM-50 ppm (437g plant-1) followed by Pseudomonas fluorescens talc based formulation (2%) (233 g plant-1)among the treatments.