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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Phytochemical analysis and antioxidant potential of banana (Musa spp)(Department of Home Science, College of Agriculture, Vellayani, 2016) Siji, S; KAU; Nandini, P VThe research project entitled “Phytochemical analysis and antioxidant potential of banana (Musa spp.)” was conducted at College of Agriculture, Vellayani, Trivandrum during 2014-2016 with objectives to study the phytochemical, nutrient, chemical composition and antioxidant potential of the selected banana varieties and to assess its therapeutic value. Eight varieties of banana viz., Palayankodan, Rasakadali, Poovan, Robusta, Red banana, Nendran, Kadali and Padatti were selected for the study. The banana varieties were procured (when the characteristic fruit colour develops) from Instructional Farm, Vellayani or from local markets of Trivandrum. The chemical and nutrient composition, phytochemical and antioxidant activity, sensory evaluation and therapeutic value of the selected banana varieties were ascertained. Results of chemical and nutrient composition revealed that TSS of banana varieties was ranged between 17.830 Brix to 23.900 Brix. TSS was found to be more in Kadali (23.900 Brix) followed by Rasakadali (23.830 Brix) and Nendran (220 Brix). Significant differences were noticed in the acidity of banana varieties studied. The varieties Kadali (0.46%) and Robusta (0.47%) were found to be less acidic. Maximum Acidity was noticed in Poovan (1.28%). The varieties Kadali (0.46%) and Robusta (0.47%) were found to be less acidic. The variety Nendran exhibited highest carbohydrate content (41.33g/100g) where as protein content was found to be higher in variety Poovan (1.37g/100g). Total mineral content of banana varieties ranged between 0.17g- 0.70g/100g and varieties such as Rasakadali (260 mg/100g) and Nendran ( 546.66 mg/100g) exhibited highest content of Na and K respectively. The calcium content of the selected banana varieties ranged between 0.35-1.35 mg/ 100g. Quantitative estimation of phytochemicals revealed that total alkaloid content of the banana varieties was observed to range between 0.84 – 3.76 per cent. The varieties Nendran (3.76%) and Padatti (3.72 %) were found to be having maximum alkaloid content. Whereas variety Rasakadali exhibited highest flavonoid content (9.49 mg/100). Saponin content of banana varieties was found to be ranged between 0.22 -0.82 per cent. The saponin content was found to be maximum in variety Kadali (0.82 per cent) and minimum in variety Padatti (0.22 per cent). Total phenol content was found to be more in varieties Kadali (11.6 mg/ 100g), Poovan (7.19 mg /100g), and Rasakadali (6.76 mg/100 g). The variety Palayankodan (4.28mg/100g) showed maximum tannin content and was significantly different from the other varieties. Antioxidant activity in the present study revealed that variety Red banana showed highest β carotene and (8.53 μg/100g). Ascorbic acid content of banana varieties ranged between 1.52 - 5.35 mg/100g. Highest ascorbic acid content was noticed in Red banana (5.35 mg). Highest dopamine content was exhibited in variety Robusta (13.3 mg/100g) and lowest was found in variety Rasakadali (3.2mg/100g). Free radical scavenging activity of banana varieties were studied by DPPH radical assay and total antioxidant activity using different solvents such as petroleum ether, methanol and water. The results revealed that variety Robusta had the highest DPPH activity with an IC50 value of 43.6 μg/ ml in petroleum ether solvent. With regard to total antioxidant activity, variety Padatti exhibited highest activity with an IC50 value of 41.2 μg/ ml in petroleum ether while variety Rasakadali (48.4) and Poovan (48.4) showed maximum activity in methanol followed by variety Red banana with an IC50 value of 44.4 μg/ ml in methanol. Sensory evaluation of eight banana varieties was done using a 5 point score card and 9 point hedonic rating scale. The results revealed that varieties Rasakadali and Red banana were most consumer acceptable varieties compared to other varieties. The therapeutic value of the banana varieties were studied by determining glycemic index. The lowest glycemic index was noticed in variety Padatti (60.70) followed by Poovan (61.00). Findings of the present study revealed that banana is rich in various health beneficial chemical components and nutrients, bioactive compounds such as ascorbic acid, beta carotene, dopamine, tannins, saponins, alkaloids and flavonoids having potent antioxidant activities and/ or free radical scavenging activity.ThesisItem Open Access Profiling bioactive compounds and nutrients in jackfruit (artocarpus heterophyllus Lam.) and developing a jackfruit based textured vegetable protein(Department of Community Science, College of Agriculture, Vellayani, 2018) Anila, H L; KAU; Suma DivakarThe investigation entitled “Profiling bioactive compounds and nutrients in Jackfruit (Artocarpus heterophyllus Lam.) and developing a jackfruit based textured vegetable protein‟‟, was carried out at College of Agriculture, Vellayani, Thiruvananthapuram during the period of 2015-2018. The objectives of the study were to ascertain the bioactive compounds and nutrients present in different jackfruit types. The study also envisaged the improvement of digestive quality of jackfruit through reduction of oligosaccharides and developing a jackfruit based textured vegetable protein. The study was conducted in three experiments; viz. analysis of nutrient and chemical profile along with antioxidant activity of the selected types of jackfruit; analysis of measures for reducing antinutrients in raw jackfruit and development of raw jackfruit based textured vegetable protein. For the component wise analysis, five types of jackfruits viz Muttom varikka, Then varikka, Sindoor, Chempikalom varikka and Local cv Koozha were selected; their raw and ripe stages as well as both bulbs and seeds were analysed separately. Analysis of proximate composition, vitamins, minerals, bioactive compounds, antinutrients and antioxidant activity were covered in the first experiment. The results showed that nutrient wise, raw seeds of Chempikalom and ripe bulbs of Sindoor scored higher than the rest of treatments while with respect to antioxidant activity, ripe seeds of Koozha were better than other treatments. The profiling of nutrients and bioactive compounds in the each part of the experiment is an eye opener on specific features of the common jackfruit type that can be exploited according to varying needs. The delicacy factor for non acceptability of jackfruit, despite its useful composition is that, it has a flatulence factor. This study has thrown light in to the measures to reduce these causative factors. For this purpose in the second part of the experiment, one treatment is with enzyme α galactosidase and another treatment with Saccharomyces cerevisiae was carried out on milled raw jackfruit bulbs and seeds of cv koozha to reduce the level of anti nutrients. Enzyme α galactosidase was premixed with the dry flour of jackfruit seed and jackfruit bulb separately in the ratio 1:100 and the moisture level was varied from 25 – 200% (dough to batter stage). The hydrolysis was carried out for 90 minutes in both jackfruit bulb flour and seed flour. The products were evaluated for the breakdown of oligosaccharides (Raffinose) using HPTLC method. The results showed that the level of Raffinose after treatment with bulb flour and seed flour was seen to decrease with increase in moisture content (25-100%). Thereafter the content slightly staggered and then reduced (125%, 150%, 175%, 200%). However the level of oligosaccharide decreased in comparison to levels in control (0.97 μg g-1). When flours were to be made into batter and subjected to fermentation with Saccharomyces cerevisiae @ 5g/kg for 6 hrs, 8 hrs and 12 hrs and analyzed through HPTLC assay; raffinose content in jackfruit bulb flour reduced from 0.75 μg g-1 to 0.63 μg g-1, 0.58 μg g-1 and 0.74 μg g-1 after 6, 8 and 12 hours respectively. Raffinose content in jackfruit seed flour reduced from 1.28 μg g-1 to 0.42 μg g-1, 0.31 μg g-1 and 0.62 μg g-1 respectively after 6, 8 and 12 hours of fermentation. Considering the reduction of raffinose content and sensory evaluation of the treated flour, eight hour fermentation (F2) was selected as the best treatment. The demand for convenience foods among the literate consumers is on the rise around the globe. It has been argued that convenience is a barrier to achieving proper nutrition using adequate servings. In order to incorporate the fruit based nutritional benefits, it has become important to develop newer and novel foods that could reach the consumers‟ acceptance. With this background an attempt was visualized to develop jackfruit based textured vegetable protein (TVP) to make jackfruit more popular among the health conscious people. In the third experiment, Jackfruit based TVP was formulated by using the ingredients - jackfruit bulb flour and seed flour along with gluten, yeast and soya flour to form chunks using standardized methods. Totally eleven combinations of TVP were worked out. All the eleven treatments were cooked and evaluated by a panel of ten members. Based on the sensory attributes such as appearance, colour, flavour, texture, taste and overall acceptability, the highest score was obtained for T7 which was followed by T8 and these two treatments were seen to be on par. The lowest score was obtained by treatment T4; T7 was taken up for quality analysis, it had a protein content of 61.50g, carbohydrate content of 34.97g and lesser cooking time (7.33 minutes). The physico chemical and textural qualities were on par with soyachunks available in the market. Processed TVP showed good storage stability up to three months. The third part of the study concluded with a positive note on scope of commercializing this jackfruit based protein concentrate. The product is both novel and healthy, raising its popularity for sailing up. Thus, the study finds that there is variation in jackfruit types with respect to nutrients, chemical and bioactive compounds. The efficacy of enzyme α galactosidase and Saccharomyces cerevisiae to reduce oligosaccharide levels in jackfruit flour is feasible. With high acceptable food like meat analogue was also standardized based on jackfruit flours, which can be recommended for commercialization.ThesisItem Open Access Nutritional profile of preschool children belonging to tribal families in Thrissur district(Department of Home Science, College of Horticulture, Vellanikkara, 2016) Vidya, T A; KAU; Seeja Thomachan, PanjikkaranThe study entitled “Nutritional profile of preschool children belonging to tribal families in Thrissur district” was conducted to know the socio-economic status and food consumption pattern of the tribal families. The study also assessed the dietary habits and nutritional status of preschool children belonging to the age group of 4 to 6 years. Sample consisted of 75 preschool children of 4 to 6 years in the tribal areas of Thrissur district. The subset of tribal and non tribal families constituting 15 tribals and 25 non tribals were used for comparative evaluation of anthropometric measurements, clinical examination, food weighment and haemoglobin estimation. Nuclear family system was found in most of the families. All the families were literate. All the families lived in their own houses and majority had 2 rooms (68.00 %) with mud flooring (58.67 %) and terraced roofing (70.67%). Majority of the families earned a monthly income between Rs. 1000 - 2000. Majority of the children had low birth weight of less than 2.5 kg. Sixty four per cent of the children suffered from various types of diseases during the last one year. Immunization of the children was ascertained. Food consumption survey revealed that all of them were habitual non vegetarians with rice as their staple food. Pulses, roots and tubers and other vegetables were included frequently in their daily diet and the use of green leafy vegetables, milk and milk products and fruits were occasional. Different grades of malnutrition were identified among the children using anthropometric indicators. The mean height and weight of the preschool children were lower than the ICMR standards and the deviations were statistically significant. It was also found that the boys were taller and heavier than the girls. Mean head circumference, chest circumference and MU AC in comparison with NFI standards were significantly lower in boys as well as girls. Height for age which reflects the past nutritional status was normal only in 35.70 per cent boys and 27.70 per cent girls. The present nutritional status of the child represented by the weight for age showed mild and moderate malnourishment in 78.60 per cent boys and 87.20 per cent girls. The Quetelet index also showed prevalence of malnutrition in 60.71 per cent boys and 59.57 per cent girls. The nutritional status of the tribal preschool children was compared with the non tribal children and the tribal children were found to be more malnourished than the non tribal children. The mean height, weight, head circumference, chest circumference and MUAC were lower in all the children, with the tribes having lower measurements than the non tribals. The height for age, weight for age and Quetelet index showed higher level of malnutrition in tribal children as against the non tribal children. Food weighment survey was also carried out to know the actual food and nutrient intake. The actual food and nutrient intake asserted that all the children had an intake lesser than the RDA. Gross deficit in the intake of certain food groups such as milk and fruits and nutrients such as iron, vitamin A and vitamin C were noticed among all the preschool children. The food and nutrient intake of tribal children were lower than that of the non tribal children. Mild anaemia was noticed among 66.67 per cent of tribal and 40 per cent of non tribal children. Food security among the tribal families recorded as per USDA module revealed 64 per cent of the families as food secure and 32 per cent of the families as food insecure without hunger and 4 per cent as food insecure with moderate hunger. The present study thus assure the fact that malnourishment is still a major concern among the tribal preschool children.ThesisItem Open Access Quality assessment and development of product from annona(Annona spp.)(Department of Home Science, College of Agriculture, Vellayani, 2016) Aswathy, K M; KAU; Nirmala, CThe research project entitled “Quality assessment and development of product from Annona (Annonaspp.)” was conducted at College of Agriculture, Vellayani, Thiruvananthapuram during 2014-2016 with the objective to analyze the physico chemical and nutritional composition of Annonaceous fruits and to develop a value added product. Three spp. of fruits viz., Bullocks heart (Annona reticulata), Sweetsop (Annona squamosa)andSoursop (Annona muricata)were selected for the study. The quality of fresh fruits was assessed in terms of physical characteristics, sensory acceptability, nutrient analysis, chemical components and shelf stability. The Total Antioxidant Activity (TAA) was also studied. Fruit nectar was standardized from the extracted pulp at different proportions of 20, 25, 30, 35 per cent level and the best proportion was identified based on sensory scores. Physical characteristics such as fruit weight, pulp weight, seed weight and weight of inedible portion were noted. Annona squamosa was smaller in size and 4-5 fruits weighed 1 kg, while 3-4 fruits weighed 1 kg in case of Annona reticulata. Annona muricatawas comparatively large in size, 2 fruits weighed 1 - 1½ kg. Results of quality analysis of fresh fruits revealed that pulp weight, ranged between 344 g to 611 g/kg fruit. Highest pulp weight was observed in Annona muricata (611g/kg). Weight of seed (113.81g/kg) and weight of inedible portions (542.80g/kg)were higher in Annona reticulata.Sensory acceptability of the fruits were evaluated and the overall mean score for the selected fruits were found to range from 6.2 to 8.8. Maximum mean score was secured for Annona squamosa(8.8) followed by Annona muricata(8.6) and Annona reticulata (6.2). Keeping quality of the annonaceous fruits were assessed by noting their organoleptic parameters on alternate days until spoilage. It was found that all the three fruits had maximum keeping quality of seven days. Quantitative estimation of major nutrients revealed that highest content of carbohydrate, energy and fat were noticed in Annona reticulata (25.12 g, 108.40 kcal, 0.61mg/100g respectively). Whereas Annona muricata exhibited highest moisture content (82.46 %). Protein content was found to be higher in Annona squamosa (2.05 mg/100g). TSS content of the selected fruits ranged from 18 – 250 Brix. The fruits were rich in potassium, calcium, magnesium, phosphorus and iron.The fruits exhibited very low fat content. Annona reticulatawas composed of 65.09μg of β caroteneper 100 gram and Annona squamosarecorded a vitamin C content of 36.20 mg/100g.Oxalates and total phenol content was higher in Annona muricata (3.36 mg/100g, 21.72 mg/100g respectively). Acidity of the selected fruits ranged from 0.27 to 1.07 per cent. Total antioxidant activity of selected fruits were studied by using different solvents such as petroleum ether, methanol and aqueous medium. The results revealed that Annona squamosa had highest antioxidant activity with IC50 value of 32.00 μg/ ml in petroleum ether. Fruit nectar was standardized from fruit pulp. Four treatment with differentproportions of fruit pulp (per cent)20 (T1), 25 (T2), 30 (T3) and 35 (T4) were developed and initial sensory analysis was done to identify the best proportion for further storage study.Selected best proportions of the fruit nectarfromAnnona reticulata (T3), Annona squamosa (T3), Annona muricata (T4) were stored at room temperature for monitoring the shelf stability in terms of changes in sensory parameters, acidity and microbial contamination periodically. The samples were analysed at weekly interval during the storage period. The stored fruit nectar was analysed for bacteria, fungi and actinomycets. No bacteria, yeast, actinomycets growth was found during the storage periodupto three weeks.An increase in acidity was noted during storage.Shelf stability of the developed product fruit nectar was found to be three weeks at ambient temperature. Annonaceous fruits possess great nutritional and health benefits through the supply of natural antioxidants and other bioactive components. Among the three spp. Annona squamosa was observed with higher antioxidant activity.Annonaceous fruits keeps well for 7 days at ambient temperature. Acceptable fruit nectarwas developed from the fruits with three weeks shelf stability at ambient temperature. Hence promoting the cultivation in large scale and scaling up the production will help to improve the health status and economic prosperity.ThesisItem Open Access Quality analysis and culinary utilization of cashew apple (Anacardium occidentale L.)(Department of Home Science, College of Agriculture, Vellayani, 2016) Neethu, Mohan; KAU; Nirmala, CThe present investigation entitled “Quality analysis and culinary utilization of cashew apple (Anacardium occidentale L.)” was conducted at the Department of Home Science, College of Agriculture, Vellayani, during the period October 2015 – May 2016. The objective of the study was to analyze the qualities of cashew apple and to evaluate its use in culinary preparations. Cashew apple needed for the study was procured from State Seed Farm, Kottarakara, Instructional Farm, College of Agriculture, Vellayani and also from local sources. The collected Cashew apple was pre-treated to remove the astringent content by steaming for 5 minutes under pressure (T1), boiling in 2 per cent salt solution for 5 minutes (T2), immersing fruit in rice gruel extract for 30 minutes (T3), immersing fruit in 0.55 per cent gelatin solution for 10 minutes (T4) and immersing fruit in 5 per cent salt solution for 3 days with the change of salt solution daily (T5). Fresh cashew apple served as control (T6). After the pre - treatment process, samples were subjected to physical, chemical and nutrient analysis. Based on the analysis of samples the treatment with least content in astringent principles (polyphenol and tannin) and with highest sensory scores were selected and stored in glass containers under refrigeration for storage study. Physical appearance and colour, fruit flavour, texture, taste and overall acceptability of treated and fresh cashew apples were evaluated by a panel comprising of 10 members using a 5 point score card. Based on the mean scores, the best treatment was identified which was T2 (Cashew apple treated in 2 per cent salt solution for 5 minutes). Chemical analysis of fresh and treated Cashew apple observed that a reduction in the chemical constituents in treated Cashew apples compared to T6 (Fresh cashew apple). It was found thatT2was in least content in polyphenol (0.11 per cent) and tannin (0.07per cent) hence selected for further in depth study. Nutrient analysis showed that the values obtained for different nutrients were lower for treated cashew apple. T2 sample got comparatively higher values than other treatments in carbohydrate (11.30g) vitamin C (180.00mg) and potassium (75.20 mg) and was also on par with control (T6). Treated cashew apple sample T2 and control T6 were stored in glass containers under refrigeration for storage study. The study showed no significant change in moisture content of the stored samples. With respect to acidity,T6 showed significant increase but not in T2. Microbial growth was seen in T2 sample on the 5th day and in T6 sample on the 7th day of storage. Cashew apple powder was also prepared from selected treatment T2 and fresh cashew apple T6 and denoted as T2P and T6P which was also used in culinary preparations. Four types of culinary preparations such as Cashew apple gravy preparation (Masala curry), Cashew apple salad, Cashew apple sweet chutney and Cashew apple sweet meat product (payasam) were made from T2andT6. Sensory acceptability of these preparations was tested by 10 panel members using 5 point score card and preference was assessed using 9 point hedonic rating scale. These four preparations were also made using cashew apple powderT2P and T6P and evaluated. Results of the organoleptic evaluation of culinary preparations from T2, T6, T2P and T6Psecured highest mean preference score for cashew apple salad (8.8/9) prepared from T2 followed by Cashew apple payasam (8.7/9) from T6 and Cashew apple chutney (8.7/9) from T2P. Cashew apple masala curry got (8.6/9) from T2. From the above findings, it can be concluded that Cashew apple treated with 2 per cent salt solution (T2) lower the astringent principle and was found to be most suitable for culinary preparations such as sweet meat products, gravy preparations, chutneys and fresh salads.ThesisItem Open Access Assessment of nutritional status and life style diseases among different income groups(Department of Home Science, College of Agriculture, Vellayani, 2016) Priya, P; KAU; Rari, John KThe study entitled “Assessment of nutritional status and lifestyle diseases among different income groups” was carried out at the Department of Home Science, College of Agriculture, Vellayani during the period, 2014-2016. The main objective of the study was to assess food habits, nutritional status and life style diseases among different income groups and impart counselling for a healthy life. Ninety employees (male and female) 30 members in high income group (HIG), 30 members in middle income group (MIG) and 30 members in low income group (LIG) belonging to the age group of 30 to 45 years was selected randomly. The classification of respondents in to different income groups was done according to NSSO report, 2007-2008. Evaluations of the socio-economic characters of the respondents found that majority of the respondents in all groups were females. They were mostly Hindus and majority of them were married. Educational status revealed that43% of LIG respondents were studied up to high school, 40 % of MIG were PGs and 50 % of HIG got Doctorate degree. On evaluating the food habits of the respondents it was found that majority of the respondents in all the three groups were non vegetarian, majority of the respondents(57 % of LIG, 50% of MIG and 77% of HIG) had regularity in their meal timings. Whereas 57% of LIG, 54% of MIG and 27% of HIG respondents had a habit of meal skipping. Thirteen per cent of both LIG as well as MIG and 16% of HIG respondents regularly consumed food from outside. On considering the meal patterns majority of the respondents had cereal, pulse and vegetable combination for their breakfast, lunch and dinner with the inclusion of non vegetarian foods like egg and fish. The food consumption pattern indicates that consumption of fast food was very low. The nutrient intake study revealed that among the three groups energy, protein and calcium intake was below Recommended Dietary Allowance (RDA). The weight for height values showed that majority of the male and female respondents were having a value higher than the standard value. In the case of BMI also it was found that only 3% of males, and 27% female in LIG, 17% of males and 37% female in MIG and 7% of males and 30% of female respondents in HIG had normal values. The WHR of the respondents also indicated higher values than normal. The clinical status of the respondents revealed that symptoms of malnutrition in hair, skin and tongue were observed; thyroid gland enlargement and anaemia were observed. The biochemical assessment conducted by a clinical expert revealed that majority of the respondents in all groups of males (7%, 13%, 10% for LIG, MIG & HIG respectively) were having mild anaemia. Were in the case of females majority of the respondents (40%, 47%, and 30%) were observed in normal level. The blood sugar level of the respondents revealed that (83%) of the respondents in LIG & MIG and 90% of the respondents in HIG were having the normal blood sugar level. Lipid levels also showed similar trends. With regarding the blood pressure values 63 per cent of LIG, 57 per cent of MIG and 60 per cent of the HIG respondents had a normal value. When the health and morbidity pattern of the respondents were assessed, it was found that majority of the respondents in LIG (56%) were not having the habit of exercise and 50% of the respondents in both MIG and HIG were exercising irregularly. The stress pattern of the respondents revealed that majority of the respondents in LIG (27%) were experienced stress due to financial problems, in the case of MIG respondents majority of them expressed the reason for stress as both financial and family problems (20%). In the case of HIG the major reason for the stress was due to occupational problems. The sleeping pattern revealed that majority of the respondents in all groups were having an adequate amount of sleep and there was no relation between stress and sleeping pattern. The lifestyle habits of the respondents like alcoholism, smoking and pan chewing was also assessed and it was found that majority of them did not have these habits in their day today life. The morbidity pattern of the respondents indicates that majority of the respondents had occupational health problems like joint pain, back pain, muscle pain and head ache. The energy intake and energy expenditure of the respondents were assessed and it was found that although energy intake was less from the standards, energy deficit was not observed because the energy expenditure was less than the energy intake and due to the same reason the prevalence of obesity among the respondents was found to be high. An education class on the occurrence, prevalence and importance of lifestyle diseases was conducted for the respondents for a healthy life. A counselling class regarding healthy life was also imparted to the respondents. It was found that the knowledge of the respondents about lifestyle diseases had improved significantly after the conduct of the class. Hence, it can be concluded that although the prevalence of lifestyle diseases among the respondents was not obvious, the chances for their occurrence is very high, because of the hidden risk factors like grade I obesity, hyperlipidimia, prehypertension, hereditary factors and stress related lifestyle problems.ThesisItem Open Access Exploring the health potential of honey and development of a value added nutraceutical drink(Department of Home Science, College of Agriculture, Vellayani, 2015) Krishnasree, V; KAU; Mary, Ukkuru PThe present investigation entitled “Exploring the health potential of honey and development of a value added nutraceutical drink” was carried out to analyze the quality of different bee honeys in the raw as well as processed form available in Kerala and to develop a nutraceutical drink utilizing the Apis cerana bee honey, major bee honey in Kerala with an idea of value addition. The major bee honeys included in the study were Apis cerana indica, Apis mellifera, Apis dorsata, Apis florae and Trigona iridipennis. The physicochemical parameters, nutrient analysis and health promoting functions of the honeys were analyzed. The moisture content of the honeys ranged from 10.03% to 19.79% with lowest being in raw Apis dorsata honey and highest in raw Trigona iridipennis honey. The electrical conductivity of the honeys ranged from 0.26 to 0.26 mS/cm among raw honeys and from 0.13 to 0.34 mS/cm among processed honeys. Significant difference (p < 0.025) was observed in the electrical conductivity of raw honeys. The ash content of the honeys was found to be 0.16% to 1.07% among the raw honeys and from 0.06 to 0.16% among the processed honeys. Significant reduction of ash content was noticed in Apis dorsata honey. The reducing sugars percentage among the raw honeys ranged from 33.03% to 67.6% and from 54.34% to 62.9%. The raw Apis florea honey varied significantly from all the other honeys in the reducing sugars. Significant difference (p < 0.025) was also noted among the processed Apis mellifera and Apis dorsata honeys. The sucrose content among the honeys varies from 5.31% to 19.73% and significant difference was observed among the raw Apis cerana and Apis dorsata honeys at p < 0.025 level. The fructose glucose ratio among the raw honeys ranged from 1.05 to 1.66 and from 1.15 to 1.71 among the processed honeys. The free acidity of the honeys ranged from 0.15% to 0.68% and all the honeys were significantly different (p < 0.025) in acidity. The pH of the honeys ranged from 3.49 to 4.45 and all the honeys under the study varied significantly (p < 0.025). The specific gravity of the raw honeys ranged from 1.28 to 1.40 which varied significantly at p < 0.025 level, while there was no significant difference among the processed Apis cerana, Apis dorsata and Apis mellifera honeys. The major quality criteria utilized to analyze the extent of processing did to the raw honey was estimated in terms of the amount of hydroxymethyl furfural content and diastase activity. The hydroxymethyl furfural content of the raw honeys ranged from 3.4 to 30.01mg and from 6.36 to 51.87mg among the processed honeys. Significant increase in the HMF content was noticed among the processed honeys. The diastase activity of the raw honeys ranged from 36.7 to 57.5 DN and from 27.32 to 54.26 DN among the processed honeys. Significant difference in the diastase activity among the raw and processed honeys at p < 0.025 level and also between the raw and processed form (at 1%) of each honey was observed. Physico chemical parameters of honeys studied was compared with the cutoff limits as quality criteria set by European Union (EU) council. Moisture and electrical conductivity were within the limits as specified by EU, while slight elevation was noticed in honeys viz., Apis mellifera and Apis florea in their ash content, sucrose content and acidity. Hydroxymethyl furfural content and diastase activity of the honeys were within the levels of EU directive. The colour intensity of the honeys were determined in terms of the chromatic components and revealed that Apis florea, raw Apis Trigona, processed Apis mellifera, raw and processed Apis dorsata honeys had lower luminance values exhibiting amber colour while all the other honeys had lighter colour showing the prominence of other tones in them. With regards to the ‘a’ and ‘b’ values all the honeys were having reddish tone and yellowish chroma. Visco elastic properties of the honeys depicted that raw and processed Apis cerana, Apis mellifera, Apis dorsata and Trigona had viscous property with high loss modulus (G'') values and Apis florea honey exhibited slight elastic property with high storage modulus (G') value. Apart from the physico chemical characteristics the microbial contamination of the honeys were also analyzed with aim to analyze the quality of the honey. The microbial analysis indicated that none of the honeys analyzed were infested with pathogenic coliforms. The sensory evaluation of the raw and processed honeys revealed that raw Apis dorsata was highly acceptable (7.35) and processed Apis dorsata was least acceptable (1.05). The most acceptable colour was for raw Apis mellifera honey (6.50) and texture for processed Apis cerana honey (5.95). Significant difference (at 5%) was noted in the sensory parameters among all the honeys analyzed. The free choice profiling technique was utilized to know the distinctive character of each sensory attribute exhibited by the honeys. The presence of amber colour was noted in Apis florea, raw Apis mellifera, raw Trigona and processed Trigona. Fruity aroma was detected in raw and processed Apis cerana, while medicinal aroma was prominent in Apis florea and all other honeys had floral aroma with an exemption to processed Apis dorsata which yielded a fermented aroma. The nutrients in the honeys were analyzed and the energy content of the honeys ranged from 328 to 353kcal and carbohydrate content from 80.25g to 88g per 100g of the honey sample. The protein content of the honeys was negligible and the major amino acid proline was detected only in raw and processed Apis dorsata (4.5mg each) and Trigona iridipennis (8mg and 3mg) honeys. The vitamin C was found to be negligible in the honeys, while vitamin A and E were not detected. With respect to the mineral content potassium was detected at higher levels among all the honeys, which ranged from 30.5 to 52mg/100g among the raw honeys and from 28 to 47.5mg /100g among the processed honeys. The iron content was noted to be from 0.54 to 1.4mg/100g among the raw honeys and from 0.24 to 1.3mg/100g among the processed honeys, while the calcium, sodium and phosphorous were also recorded to be very less. The trace elements analyzed were zinc (0.04 to 5mg/100g), copper (0.002 to 0.13mg/100g) and manganese (0.001 to 0.35mg/100g) and it was noticed that all the level of three elements were in traces in all the honeys analyzed, but they are involved in umpteen biochemical functions in the body as metalloenzymes. Hence the presence of trace elements even in marginal levels might exert therapeutic properties to the honeys. The health promoting functions of the honeys were analyzed which includes the phytochemical profiling, antioxidant activity, antidiabetic activity and antimicrobial activity. The phytochemicals analyzed were polyphenols, flavonoids and flavonols. Among the raw honeys polyphenols were high in Apis dorsata (1168mg/kg) and low in Apis mellifera (905.25mg/kg); flavonoids were high in Apis dorsata (545.75mg/kg) and low in Apis cerana (185mg/kg) and flavonols were high in Trigona iridipennis honey (17.6mg/kg) and low in Apis mellifera (4.64mg/kg) and Apis florea (4.6mg/kg) honeys. Significant difference was noticed among all the honeys analyzed with respect to the phytochemicals and significant reduction in the phytochemicals was also observed on processing among all the honeys. The total antioxidant activity of the honeys was found to be highest in raw and processed Trigona (99.22%, 93.73% at 500µg/ml) honey followed by Apis cerana (97.04% at 500µg/ml) honey among the raw honeys and Apis mellifera (93.4% at 500µg/ml) among the processed honeys respectively, while moderate level of activity was noted in raw and processed Apis dorsata with 73.64% and 64.84% of antioxidant activity at at 500µg/ml. The DPPH radical scavenging activity was found to be highest in raw and processed Trigona honey (97.21%, 92.56% at 500µg/ml) followed by raw Apis cerana (95.55% at 500µg/ml) and processed Apis mellifera (88.18% at 500µg/ml) honeys, whereas comparatively lesser scavenging activity was viewed in processed Apis dorsata honey (48.17% at 500µg/ml). Average scavenging activities were noted in raw Apis dorsata and Apis florea. Significant differences (p < 0.025) in the radical scavenging activity were observed among all the raw honeys analyzed. On contrary to the above discussed antioxidant activities, non linearity was observed among the honeys in the nitric oxide scavenging activity. The highest nitric oxide scavenging activity was observed in raw Apis mellifera (77.19% at 500µg/ml) followed by Apis dorsata (68.22% at 500µg/ml) and Apis florea (65.35% at 500µg/ml) honeys. The antidiabetic activity determined in terms of alpha amylase inhibition capacity showed highest inhibition in raw and processed Trigona (77.61% and 64.84% at 500µg/ml) honeys followed by raw Apis dorsata (44.34% at 500µg/ml) honey. Average enzyme inhibition was observed among raw Apis mellifera (41.32% at 500µg/ml) and Apis florea (41.30% at 500µg/ml) honeys correspondingly. Higher rate of alpha glucosidase was also observed among the honeys ranged from 76.14% to 80.46% among the raw honeys and from 69.17% to 78.29% among the processed honeys. Significant (at 5%) reduction in the percentage inhibition was noticed in processed honeys. The glycemic index (≤ 67) and glycemic load (≤ 13.54) of the honeys revealed that all the honeys belong to intermediate GI foods category. The antimicrobial activity of the honeys analyzed revealed that raw honeys had higher antimicrobial activity than the processed form with highest in raw Trigona followed by Apis mellifera and Apis cerana honeys. From the elaborative analysis on the health promoting function of the bee honeys it could be concluded that raw honeys have higher health boosting properties when compared to the processed honeys. The screening index in terms of the therapeutic potencies showed that Trigona iridipennis honey had the highest therapeutic potency followed by Apis mellifera honey, while comparatively moderate potency was observed in Apis dorsata and Apis cerana honeys. A value added nutraceutical drink was developed utilizing the Apis cerana bee honey, the most abundantly available honey in Kerala. The drink was formulated by working out different proportions of honey in combination with aloe vera gel. The bitterness of the aloe gel was removed by trying out different pre treatment combinations. The physico chemical characteristics of the drink revealed that the drink is mildly acidic in nature with 61% moisture, total soluble solids (35°Brix), acidity (0.34%) and pH (4.88). The total and reducing sugars of the nutraceutical drink were 29.69% and 18.62% respectively. The fructose to glucose ratio of the nutraceutical drink was observed to be 0.99. The drink was found to have high therapeutic potency with 1060 (mg gallic acid/kg drink) of polyphenols, 60 (mg quercitin/Kg drink) of flavonoids and 5 (mg catechin/Kg drink) of flavonols respectively. Remarkable in vitro total antioxidant capapcity (72.1µgAAE/mg at 500µg/ml), DPPH radical scavenging activity (58.4% at 500µg/ml) and alpha amylase inhibition capacity (43% at 500µg/ml) with a medium glycemic index of 65 was also determined. Even though, the drink exhibited a slight increase in the acidity and TSS on storage the drink was noticed to be free from microbial proliferation and was acceptable till the end of the storage period, indicating its fitness for consumption even after two months of storage. The cost of the drink was found to be Rs 22.00 per 200ml and yield ratio was 1.49. The clinical efficacy of the drink was determined through a supplementation study which inferred that the platelet count increased significantly (at 5%) in the subjects from 2.56± 0.02 lakh/cumm to 3.05 ± 0.04 lakh/cumm. The supplementation had resulted in improved haemoglobin levels of the respondents from 10.83±0.06 gm% prior the study to 12.01± 0.02gm% at the end of the study. Significant improvement was also recorded in the hematological indices viz., WBC (from 7310± 114.3 to 8330 ± 43.7 cells/cumm), RBC (from 4.16 ± 0.05 to4.48±0.03 millions/ cumm), MCV (88.06 fL to 92.36 fL) and PCV (36.24±0.54% to 39.78± 0.42%) under the study. Encouraging results were also obtained from the total antioxidant capacity of the serum of the subjects under investigation. Findings of the present study strongly recommend that bee honeys are indeed the elixir of life with admissible physico chemical characteristics and superior health promoting functions. Among the honeys analyzed the raw honeys (Apis cerana, Apis mellifera, Apis dorsata, Apis florea and Trigona iridipennis) were found to be better in terms of quality as well as therapeutic potency when compared with the processed honeys. Among the raw honeys Trigona iridipennis honey was found to the best in medicinal value. In vitro and in vivo studies prove the efficacy of the developed drink to be a novel option in the market of nutraceuticals with superior health boosting properties and being natural by all means.ThesisItem Open Access Quality evaluation of germinated rice and rice products(Department of Home Science, College of Horticulture, Vellanikkara, 2012) Neethu Sathyan, T; KAU; Suman, K TThe study entitled “Quality evaluation of germinated rice and rice products” was undertaken to evaluate the physical, cooking, biochemical, nutritional, organoleptic and keeping qualities of germinated rice. The study also aimed to evaluate the physical and keeping qualities of roasted rice flour and to assess the acceptability of the products prepared with rice flour. The rice variety Jyothi was selected for the study. After soaking for 12hours, it was germinated under laboratory conditions. The treatments were T0 – Ungerminated rice, T1- Rice- soaked for 12 hours, T2 – Rice- soaked and kept for germination for 3 days and T3 – Rice- soaked and kept for germination for 6 days. Milling recovery was found to be very low in germinated rice which in turn resulted in higher milling loss. Total milled rice and head rice recovery of germinated rice decreased significantly during storage when compared to control. Significant variation among treatments was observed with respect to thousand grain weight and thousand grain volume. To obtain optimum cooked rice, a cooking time of 22.33 to 29.33 minutes was noticed among different treatments. A positive correlation between water uptake, volume expansion and grain elongation was noticed in all the treatments. Germination decreased the amylose content thereby affecting the gel consistency. Intermediate gelatinisation temperature index was noticed in all the treatments. Moisture content of rice from four treatments varied from 10.51 to 12.67 per cent which increased significantly during storage. A significant increase in total and reducing sugar was observed as a result of starch degradation during germination. Variations were noticed in the protein, fat and fibre content among treatments. Progressive increase in the thiamine content was observed with advancement in days of germination. Compared to control, calcium, iron and phosphorus content of germinated rice were significantly low. During germination, in vitro starch digestibility of rice from different treatments increased by 5 to 17 per cent. Significant increase in in vitro mineral availability was also noticed. Bulk density of roasted rice flour from different treatments varied from 0.78 to 0.84 g per ml. Compared to control, lower water absorption index and higher water solubility index was noticed in roasted rice flour from germinated rice. The evaluation of retrogradation property in roasted rice flour from different treatments revealed that syneresis per cent increased with advancement in days of observation. Maximum syneresis was noticed in germinated rice. The products prepared using rice (cooked rice, unniappam and kozhukatta) and rice flour (iddiappam and puttu) from germinated paddy had mean scores above 6.0 for different quality attributes. Germinated rice was found to be more suitable for the preparation of unniappam and puttu. Mean scores for various quality attributes increased when the products were prepared from stored rice and rice flour. The rice and roasted rice flour were evaluated for the presence of bacteria, fungi and yeast initially and after three months of storage. Presence of bacteria was detected in both periods under study. Fungal growth was not noticed in any of the rice and rice flour samples initially but after three months of storage fungal growth was detected in roasted rice flour of T0 and T1. Yeast count was not detected in rice and rice flour initially and after three months of storage. Insect infestation was not noticed in rice and rice flour from different treatments throughout the storage period.ThesisItem Open Access Quality evaluation of newly released KAU rice (Oryza sativa L.) varieties and their suitability for traditional food products(Department of Home Science, College of Horticulture, Vellanikkara, 2015) Chandhni, A A; KAU; Aneena, E RThe present study entitled „Quality evaluation of newly released KAU rice (Oryza sativa L.) varieties and their suitability for traditional food products‟ was conducted to evaluate the physical, biochemical, nutritional, cooking and organoleptic qualities of newly released KAU rice varieties. The study also aimed to assess the suitability of these rice varieties for the preparation of selected traditional food products. Milling per cent among rice varieties varied from 64.07 per cent in Prathyasha (MO-21) to 77.83 per cent in Ezhome-2. Vyttila-8 obtained the highest head rice recovery of 57.45 per cent while it was lowest in control variety Jyothi (41.08 per cent). The highest thousand grain weight (20.68 g) and volume weight (14.6 mm3) was observed in Vaishak (PTB-60). Based on the L/B ratio, the grain shape of Ezhome-1, Vyttila-8, Vaishak (PTB-60) and Uma were classified as bold medium. Ezhome-2, Prathyasha (MO-21) and Jyothi varieties were categorised as slender long grains. For most of the physical quality parameters, raw rice of newly released varieties were found to be superior over the control varieties Jyothi and Uma. Newly released rice varieties obtained lower total index for organoleptic qualities than control varieties. Ezhome-2 obtained highest total index for table rice among newly released varieties. Among rice varieties, lowest cooking time of 18.51(Prathyasha (MO-21)) to highest of 24.75 (Ezhome-2) minutes was noticed for obtaining optimum cooked rice. Highest water uptake of 5.74 ml/ g was observed in Ezhome-2 and lowest of 3.3 ml/ g in Jyothi. Control varieties Jyothi and Uma obtained volume expansion ratio of 5.62 and 6.07 respectively, which was higher than that of the newly released rice varieties. Among rice varieties, amylose content varied from 10.20 per cent in Prathyasha (MO-21) to 23.32 per cent in control variety Uma. Gel consistency of different rice varieties was determined and it varied from 27.50 to 145 mm. Cooking qualities of the newly released rice varieties were found to be comparable with that of the control varieties. Among newly released rice varieties, higher grain elongation ratio of 0.90 was noticed in both Ezhome-2 and Prathyasha (MO-21). Maximum keeping quality up to 12.60 hours was noticed in Vaishak (PTB-60) and minimum of 11.24 hours in Prathyasha (MO-21). The moisture content of different rice varieties ranged from 8.5 per cent in Ezhome-2 to 11.6 per cent in Ezhome -1 and Prathyasa (MO-21). The highest total carbohydrate, starch, energy, protein, zinc and phosphorous content was noticed in newly released rice variety Ezhome-2. Fat content in different rice varieties ranged from 0.30 per cent in Ezhome-2 to 0.71 per cent in Vaishak (PTB-60). The highest fibre content of 0.7 g/ 100g was noticed in Ezhome-1. Maximum thiamine content of 0.07 mg/ 100g was noticed in Vaishak (PTB-60). The highest calcium and iron content was noticed in Jyothi and Prathyasha (MO-21) respectively. Newly released rice varieties were found to be superior to control varieties in nutritional qualities. In the case of unroasted rice flour, highest bulk density of 0.73 g per ml was noticed in Ezhome-2 and the lowest of 0.63 g per ml in Jyothi. In roasted rice flour, the highest bulk density of 0.62 g per ml was observed in control variety Uma. The highest water absorption index of 25.46 was observed in unroasted rice flour of variety Jyothi (control) and 25.11in roasted rice flour of Uma (control). Highest water solubility index of 0.78 was observed in unroasted rice flour of Vyttila-8 and the lowest of 0.34 in Ezhome-1. In roasted rice flour the highest water solubility index of 0.81 was noticed in Ezhome-2 and the lowest in Vyttila-8 (0.28). At the end of the 12th day of study the highest synerisis per cent in unroasted rice flour was noticed in Vyttila-8 and that of roasted rice flour in Ezhome-1. Physical properties of rice flour of newly released rice varieties were comparable with that of the control varieties. Evaluation of microbial qualities indicated that at the end of 6th month of storage bacterial count and fungal growth was observed in raw and roasted flour of different rice variety. Yeast was not observed in any of the raw rice and roasted rice flour of different varieties till 5th month of study. Vyttila-8 was found to be the most suitable rice variety for the preparation of rice flakes and unniyappam. The most suitable variety for the preparation of cooked rice and iddli was control varieties Jyothi and Uma respectively. Prathyasha (MO-21) was found to be the most suitable variety for the preparation of appam and ada and is also suited for idli. Vaishak (PTB-60) was the most suitable rice variety for puttu. In the present study, It was observed that newly released red rice varieties were suitable for the preparation of different traditional food products.
