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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Bacteriological quality of goat milk(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1985) Chakhesang, Kevesiei; KAU; Sukumaran, M VA total of 376 aseptically – drawn milk and 46 farm pooled milk samples were collected from the AICRP on Goats for Milk, Mannuthy and subjected to various tests to determine the bacteriological quality. An increase of over 59 and 188 – fold in bacterial number from aseptically – drawn milk to production – and milk for the morning and the evening milkings respectively was noticed. In aseptically – drawn milk samples 61.17% and 64.83% gave a SPC of below 100/ml in the morning and evening respectively. In farm pooled milk samples the SPC exceeded 105/ml in 39.13% of the morning and 65.22% of the evening samples. No psychrophilic bacteria was detected in any of the aseptically – drawn or farm pooled milk samples. The mean thermophilic counts of aseptically – drawn as well as farm pooled milk samples was less than one/ml. Though the mean coliform count of aseptically – drawn milk was found to be less than one/ml, the farm pooled milk samples showed 3,413 and 2734/ml for the morning and the evening samples respectively. The mean MBRT of the morning and the evening samples was 14.91 and 8.93 h for aseptically – drawn milk and 4.57 and 3.83 h for farm pooled milk samples respectively. The correlation coefficient between MBR time and SPC of farm pooled milk of the morning and evening samples was not significant (- 0.21 and - 0.27). The keeping quality of the morning and the evening milk samples (280 C) obtained was respectively 50.48 and 44.70 h for aseptically – drawn milk and 12.87 and 12.04 h for farm pooled milk samples respectively. The correlation coefficient between SPC and keeping quality of farm pooled milk samples was significant (- 0.46) for morning sample while that of evening milk was not significant (+ 0.28). The correlation coefficient between MBRT and keeping quality of farm pooled milk samples was also not significant in both the morning and the evening (+ 0.31 and + 0.13).ThesisItem Open Access Qualitative changes of yoghurt prepared from milk preserved by different methods(Department of Dairy Science, College of Veterinary and Animal Sciences,Mannuthy, 1990) Prasad, V; KAU; Sukumaran, M VAn experiment was conducted to study the qualitative changes of yoghurt prepared from milk preserved by different methods. An attempt was also made to study the efficiency of LP system in controlling psychrotrops in refrigerated milk thereby extending the keeping quality of such stored milk. The efficacy of the system was compared with pasteurized milk and raw milk stored under similar conditions. An exhaustive review of literature has been presented on the use of various preservation methods employed in milk, quality of yoghurt and other related aspects. The methods of analyses of some important components of milk and yoghurt has been detailed. The milk after collection were divided into three portions and used for various treatments A (raw milk as such), B (laboratory pasteurized) and C )LP activated). Samples in each treatment were again divided into four parts and stored under refrigeration for 0, 24, 48 and 72 hours for further analysis and yoghurt preparation. When raw milk was stored under refrigeration, acidity developed to a significantly high level (P < 0.05) at the end of 72 hours of storage whereas no significant increase in activity was noticed in treatments B and C. This result was corroborated by a decrease in both psychrotrophic and total bacterial counts in milk under treatments B and C. So it was concluded that the development of acidity in treatment A could be due to bultiplication of microbes particularly psychrotrophs. In B and C its growth was arrested by the treatments. Hydrogen peroxide and thiocyanate were detected in all raw milk samples at a level of 3.78 and 7.33 ppm respectively. The level of H2 O2 and SCN – used for the activation of LP system for the study were found to be adequate, since the psychrotrophic count in milk at the end of 72 hours in treatment C was less than that found in treatment B. The residual H2 O2 and SCN – contents at the end of 72 hours were found to be within the normal level found in milk thus having no health hazards, by this method of preservation. When yoghurt samples were prepared from stored milk no significant differences between treatments were noticed in the values of fermentation period, acidity, pH, total proteins and total solids. A significant increase in NPN and tyrosine values were noticed in yoghurt prepared from milk under treatment A indicating extensive proteolysis in milk during storage under this treatment. However, no difference was noticed between treatments B and C. Diacetyl and acetaldehyde were produced at a desirable level in yoghurt samples under all the three treatments. A proper ratio of Str. Thermophiles – 6 and L. bulgaricus – 4 was found to be maintained in all yoghurt samples. On organoleptic evaluation the yoghurt prepared from milk under treatment C could not be distinguished from other two treatments. In fact, yoghurt under C got a higher total score on organoleptic evaluation than A and B. Low score for body and texture, and falvour was observed under treatment A. This may be attributed to the effect of growth of psychrotrophs in raw milk during refrigeration. From the results, it was concluded that good quality yoghurt can be prepared from milk stored under refrigeration following pasteurization or LP activation. Yoghurt samples prepared from milk stored under treatment A was found to be of inferior quality when compared to B and C. The result also confirmed that LP system can be recommended as a safe preservative for extending storage life of refrigerated milk. When such stored milk was used for yoghurt production, no significant difference in the quality was noticed when compared to yoghurt under treatment B. Suggesting that LP activated milk can be conveniently and economically used for the production of fermented milk products like yoghurt without any apparent harmful effect.ThesisItem Open Access Preparation of mozzarella cheese using skim milk filled with coconut milk(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1994) Gnana Selva, Johnson; KAU; Mukundan, MA detailed study was carried out to determine the quality of coconut fat filled milk for the preparation of Mozzarella cheese and why drinks. Literatures based on filled milk products has been reviewed, apart from the preparation of cheese and why drinks. The control samples of Mozzarella cheese and whey drinks were prepared using cow’s milk. Experiment I products were prepared from milk in which 50 per cent of milk fat was replaced with coconut fat. Experiment II products were prepared from cheese milk in which 100 per cent of milk fat was replaced with coconut fat. All the samples of milk were standardized to 4 per cent fat. A total of 6 trials were carried out to obtain reliable data for statistical analysis. The acidity, pH, stretchbility and FDM content were found to be similar in control, experiment I and II Mozzarella cheese. Eventhough, the control Mozzarella cheese were found to have slightly higher yield protein, fat and lower moisture content, the experimental I and II. Mozzarella cheese also satisfied the requirements for good quality Mozzarella cheese. The control Mozzarella cheese got maximum score on sensory evaluation than the experiment I and II Mozzarella cheese. Pineapple and Lemon falvoured control, experiment I and II whey drinks were found to be equally acceptable with nodifference on storage studies ar 5 + loC. Total bacterial count on whey drinks were also made. The studies revealed that the cow milk in which the milk fat replaced to the extend of 50 per cent and 100 per cent with coconut fat can be effectively utilised for preparation of Mozzarella cheese. The quality of such cheese is comparable with that made from cow milk.ThesisItem Open Access Studies on the influence of tannins on nucleic acid and protein syntheses in ruminants(Faculty of Dairy and Animal Husbandry, Haryana, 1976) Sadanandan, K P; KAU; Arora, S PA study was conducted to elucidate the influence of tannins on synthesis of nucleic acids and protein in liver of rats. In vitro and in vivo studies in buffaloes were also conducted to ascertain the effect of tannins on rumen metabolism. In experiment 1, 30 weanling rats were distributed into three groups of 10 each in a randomized block design. The influence of addition of 0% (group A), 2.5% (group B) and 5% (group C) tennins in the feed on feed consumption, growth rate, nitrogen and dry matter digestibility was investigated. Further RNA, DNA and protein in liver were estimated to asses liver function. The feed consumed daily on DM basis (g); weight gain per three day interval (g); and gram feed per gram weight gain, respectively for groups A, B and C were : 20.05 + 7.6, 7.87 + 0.41, 2.73 +0.05; 16.66 + 6.0, 5.69 + 0.35, 3.14 + 0.07 and 16.11 + 5.4, 4.53 + 0.21, 3.80 + 0.11. The DM and N digestibility (%), respectively for groups A, B and C were: 78.56 + 0.44, 78.28 + 0.56; 78.62 + 0.64, 73.50 + 0.86 and 78.82 + 0.52, 69.97 +0.75. Feed consumption in group A was significantly (P <0.01) higher than in group B and C. The difference in feed consumption between groups B and C was not significant. Significant differences were found amongst all treatment groups in weight gain (P< 0.05) and food : gain ratios (P < 0.01). DH digestibility did not reveal any significant difference between groups whereas the differences in N-digestibility were significant (P<0.01). The addition of tennis in the diet significantly depressed feed consumption, weight gain, and N – digestibility which resulted in widened feed : gain ratios. The average liver weights (g): total protein (mg) ; RNA (mg) and DNA (mg), respectively for groups A, B and C were : 3.61 + 0.21, 717.3 + 4.76, 21.42 + 1.41, 5.36 + 0.41, 2.85 + 0.23, 569.0 + 4.31, 16.40 + 1.60, 4.29 + 0.45 and 2.44 + 0.01, 507.9 + 2.55, 13.79 + 0.58, 3.34 + 0.16. The liver weight in group A was significatly (P<0.05) higher than in group C. The total protein content in group A was significatly (P<0.01) higher than in group B and C. But the difference between groups B and C was not significant. RNA and DNA contents differed significantly (P<0.01) amongst the three groups. The average protein (mg), RNA (mg) and DNA (mg), respectively for the groups A, B and C were : 198.69 + 3.31, 5.93 + 0.18, 1.48 + 0.05, 199.89 + 5.14, 5.68 + 0.16, 1.49 + 0.06 and 208.16 + 2.32, 5.66 + 0.03, 1.38 + 0.04 per gram of tissue. There were no significant differences in the parameters studied amongst the three groups. The body weight : liver weight ratios, protein : RNA ratios and protein : DNA ratios, respectively for groups A, B and C were : 29.0 + 2.23, 33.2 + 1.01, 134.9 + 5.04, 30.3 + 2.45, 35.4 + 1.22, 136.7 + 5.75 and 31.0 + 2.66, 36.9 + 1.01, 153.7 + 4.17. There were no significant differences amongst the ratios except that protein : DNA ratio in group C was significantly (P<0.05) wider than in group A and B indicating probable hypertrophy of liver cells in that group. It was apparent that tannins exerted their harmful effects by affecting protein digestibility in the gastro-intestinal tract and thereby adversely affected liver size and growth rate. In experiment 2, in vitro trials were conducted by taking buffalo rumen liquor through a rumen fistula on a control ration without tannic acid. For N solubility and DH digestibility studies, the substrate used was : Maize, 50 parts ; grount nut cake, 21 parts and wheat bran 26 parts, ground into 40 mesh size. To study the influence of tannins on protein synthesis, nucleic acid synthesis and production of VFA, the substrates used were : cellulose 0.75 g. starch 0.25 g and ammonium sulphate 151 mg. McDougall’s artificial saliva was used as buffer (PH 6.8) for 32 P uptake by rumen microbes, the substrate was prepared from glucose 600 mg and ammonium sulphate 85mg. A mineral solution containing cysterine – HCL described by Bucholts and Bergan (1973) was used as a buffer. The levels of tannic acid, respectively in groups 1,2,3,4 and 5 were : 0, 1.25, 2.5, 5.0 and 7.5% in all the experiments. The E solubility and DM digestibility (%) respectively, for treatments 1,2,3,4 and 5 were : 36.72 + 0.425, 43.80 + 2.63 : 24.48 + 0.311, 37.60 + 2.14, 20.81 + 0.589, 30.27 + 1.85 : 17.55 + 0.312, 21.89 + 1.93 and 15.30 + 0.473, 13.20 + 1.15. Addition of tannins depressed N solubility and DM digestibility. The protein – N (mg) ; RNA – N (mg): DNA-N (mg) and TVFA (meq) (all per 100 ml) respectively for treatments 1,2,3,4 and 5 were : 30.19 + 1.274, 2.156 +0.107, 0.795 + 0.054, 15.46 + 0.315, 23.84 + 1.021, 1.565 + 0.101, 0.561 + 0.025, 12.34 + 0.194, 18.59 + 0.582, 1.185 + 0.046, 0.426 +0.021, 9.38 + 0.425, 16.27 + 1.318, 1.00 + 0.042; 0.337 + 0.013, 7.29 + 0.359 and 14.61 + 0.271, 0.865 + 0.034, 0.290 + 0.006, 5.49 + 0.235. Addition of tannins significantly (p<0.01) depressed all the parameters studied and in treatment 5, the levels were more or less the same as in 0 hour control indicating complete inhibition of microbial multiplication at 7.5% tannic acid level. The RNA –N : protein – N, DNA – N: protein – N and total nucleic acid – N: protein – N ratios respectively for treatments 1,2,3,4 and 5 were : 0.072 + 0.0018, 0.026+ 0.0013, 0.098 + 0.0015, 0.066 + 0.0018, 0.023 + 0.0045, 0.089 + 0.0022; 0.064 + 0.0018, 0.023 +0.00084, 0.087 + 0.0024, 0.062 + 0.0016, 0.021 + 0.00077, 0.082 +0.0020 and 0.058 + 0.0013, 0.020 + 0.00055, 0.078 + 0.0016. The ratios were narrower in control group when compared to tannic acid groups. With regards to 32P uptake by rumen microbes, a progressive decrease was observed with increase in tannin concentration. 32p uptake (mg) per 100 ml respectively for groups 1,2,3 and 4 were : 2.640, 1.835, 1.202 and 0.52. In group 5 there was no 32P uptake. Tannins depressed microbial multiplication indirectly by making the protein source not available due to its precipitation. Direct harmful effect was also possible on microbes, especially, at higher concentrations of tannins in the media without any protein source. In experiment 3, four adult fistulated female buffaloes were randomly distributed in a Latin square design. The treatments I, II, III and IV respectively contained 0, 1.25, 2.5 and 5% tannins made available from 0, 14, 28 and 40% salseed meal in the ration. In treatment IV, 1.436% pure tannic acid was also added to get 5% total tannins. The DCP and TDN contents were approximately 14 and 72% in all the rations. The effect of tannins in feeds was determined through the levels of protein –N, RNA – N, DNA –N and TVFA. The protein –N, RNA – N and DNA-N levels ( all in mg per 100 ml of SRL) and TVFA levels ( meq/100 ml of SRL), respectively for treatments I,II,III and IV were : 43.73 + 1.813, 3.86 + 0.134, 1.63 + 0.053, 9.59 + 0.205; 49.087 + 1.912, 3.75 +0.115, 1.59 + 0.057, 0.43 + 0.215, 54.86 + 1.850, 3.62 + 0.089, 1.50 +0.041, 9.20 +0.188 and 61.89 + 2.050, 3.26 + 0.097, 1.37 + 0.046, 8.48 + 0.283. Protein - N level in treatment L was significantly (P< 0.05) lessthan in ratios II, III and IV and there was a progressive and significant (P< 0.05) increase in order of treatments. RNA – N and TVFA levels in treatment I were significantly higher (P< 0.01) than in treatment IV. DNA levels were significantly lesser (P<0.05) in treatment III than in treatment I and again lower in treatment IV than in treatment III. Nucleic acid - N : protein – N ratios is SRL respectively for treatments I, II, III and IV were : 0.125 + 0.0012, 0.108 + 0.0020, 0.093 + 0.0026 and 0.072 + 0.0011. The ratio in treatment L was significantly higher than in treatments II, III and IV. The differences amongst the four treatments were significant (P<0.01). Addition of tannins in the rations resulted in an increase in protein - N, but progressively depressed the RNA – N and DNA – N levels with less production of TVFA. Further in experiment 3, the protein - N, RNA – N and DNA – N contents of bacteria separated from SRL were also determined to ascertain the effect of tannins on RNA – N: protein – N; DNA – N : protein – N and total nucleic acid : protein - N ratios. Protein – N (mg), RNA – N (mg); and DNA –N (mg) in bacteria separated from 100 ml SRL respectively for treatments I, II,III and IV were : 23.93 + 0.571, 2.385 + 0.87, 1.204 + 0.036, 23.71 + 0.627, 2.296 +0.062, 1.180 + 0.019, 22.79 +0.590, 2.230 +0.044, 1.111 + 0.059 and 20.91 + 0.544, 205 + 0.046, 1.010 + 0.053. Protein -N, RNA –N and DNA – N levels decreased as levels of tannins in rations increased. But the differences were significant (P<0.01) only between treatment I and IV. RNA – N : protein - N, and total nucleic acid – N : protein – N ratios respectively for treatments I, II, III and IV were : 0.099 + 0.0030, 0.150 +0.0027; 0.097 + 0.0023, 0.147 + 0.0029; 0.098 + 0.0024, 0.147 + 0.0017 and 0.098 + 0.0020, 0.146 +0.0025. The differences in the ratios amongst the different treatments were not statistically significant. The addition of tannins at the levels tried had no significant influence on the nucleic acid – N : protein – N ratios in the bacteria. From the value obtained for nucleic acid – N and nucleic acid – N : protein – N ratios in separated bacteria, the microbial contribution of protein - N to the tungestic acid precipitate of SRL was calculated. The values obtained were : 83.67, 74.01, 63.58 and 51.14 % for treatments I,II,III and IV respectively. The tannins present is the feed partially protected the proteins from microbial attack and hence the contribution of dietary protein - N in the SRL increased. Simultaneously the quantity of microbial protein synthesis decreased due to the limitations imposed by tannins on microbial multiplication.ThesisItem Open Access Determination of solids content of milk by specific gravity lactometer(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1980) Babu, Mathew; KAU; Subramaniam, MThe methods of estimating total solids content of milk having high percentage by Richmond’s formula (TS = 0.25 L + 1.2 F + 0.14) using Quevenne’s specific gravity lactometer, modified Richmond’s formula (TS = 0.25 L + 1.2 F + 0.50) using Zeal specific gravity lactometer, ISI formula (TS = 0.25 DH + 1.22 F + 0.72) and Ling formula (TS = 0.25 DH + 1.21 F + 0.66) using the density hydrometer, along with the percentage of fat estimated by Gerber method were compared with the value obtained by gravimetric method using 307 samples of milk. In all the milk samples analysed the calculated values of total solids by the formula methods were both higher and lower than the gravimetric values. Since the formulae methods did not give a true estimate of the total solids in milk, modifications to the existing formulae were required by applying a correction factor. It was observed that depending up on the percentage of fat in milk samples, different correction factors were needed to be applied to the different formulae for getting the values close to the gravimetric method. Addition of a correction factor of 0.15 and 0.18 was necessary to the Richmond’s formula for milk samples containing 5.1 to 6.0 and 6.1 to 7.0 per cent fat respectively. For the modified Richmond’s formula, addition of 0.57, 0.53, 0.66 and 0.75 was necessary for samples containing 3.0 to 4.0, 4.1 to 5.0 and 5.1 to 6.0 and 6.1 to 7.0 per cent fat. The addition of a correction factor of 0.16 to the ISI formula was essential for samples containing 4.1 to 5.0 milk fat to get the values in agreement with the gravimetric method. No correction factor was necessary, if Ling formula was used. The order of preference for using various formulae will be (1) Ling formula (2) ISI formula (3) Richmond’s formula (4) modified Richmond’s formula. Ling formula can be used without any correction factor for determination of total solids content of milk for varying percentages of fat from 3..0 to 9.0.ThesisItem Open Access Detergent potential of enzymes of dairy microflora and their effect on the shelf life of milk products(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 2010) Beena, A K; KAU; Geevarghese, P IA study was conducted to assess the detergent potential of a spoilage protease enzyme obtained from the microflora of dairy plant environment. An attempt was also made to study the impact of selected enzyme producers on the shelf life of curd (dahi) and sterilised skim milk. A total of 71 bacterial isolates obtained from dairy environment were screened for their ability to produce spoilage enzymes like proteases lipases and lecithinases. Based on the spoilage potential, Pseudomonas aeruginosa (P12) isolated from pasteurised milk and Bacillus cereus (S4) isolated from sterilized skim milk were selected for further work. The influence of spoilage enzymes on selected physico-chemical characteristics of curd (dahi) and sterilized skim milk was evaluated by preparing the products from milk precultured with isolate P12 and S4. In general, proteolysis of milk was found to have an adverse effect on the quality of products. The stimulatory effect of proteolytic products of P12 and S4 on curd starters was evident from the higher values of acidity, firmness and syneresis in treated curd. The spoilage enzymes adversely affected the overall quality and shelf life of curd. In treated sterilised milk, tyrosine and NPN values were highly elevated. A linear correlation was found to exist between off-flavour and proteolysis. Curd and sterilised skim milk prepared from milk precultured with proteolytic organism were significantly different from that of control. The possibility of exploiting an alkaline protease from spoilage organism in dairy plant sanitation was also looked into. Environmental conditions for the production of alkaline protease by a psychrotrophic strain of Bacillus cereus (S4) was optimised in whey based medium. The protease used in this trial preferred an alkaline medium to remain stable. The enzyme was found to be stable over a wide temperature range of -10°C to 80°C and a pH range of 7.0 to 12.0. The metal ions Ca++, Mg++, Zn++ and Hg++ enhanced the enzyme activity. Lack of inhibition by Hg++ suggested lack of disulphide bonds in the active site of enzyme. Significant inhibition of activity by serine inhibitors indicated an essential serine residue in the active site of enzyme. The deleterious effect of EDTA on enzyme activity showed the supportive role of divalent cations. Marked residual activity on treatment with β-mercaptoethanol indicated the absence of cysteine residue for the enzyme. Enhancement of protease activity in the presence of surfactants and stability in the presence of H2O2 signified its potential to be used as detergent additive. Qualitative assessment of cleaning efficiency of inbuilt formulation substantiated the superiority of enzyme based formulations. Ammonium sulphate fractionation, dialysis and gel filtation using seralose 4B and Seralose 6B were effective in purifying the protease preparation by 141.31 fold. The purified protease was found to be a homogenous preparation of molecular weight of 50.5 kDa as determined by SDS PAGE.ThesisItem Open Access Development and evaluation of different vaccines against duck pasteurellosis(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 2010) Jesto, George; KAU; Krishnan NairThis study was undertaken to develop biofilm vaccines against duck pasteurellosis using oil, saponin and aluminium hydroxide as adjuvants and to experimentally evaluate their immunogenicity in ducks. Identity of P. multocida serotype A: 1 (DP1) used for study was confirmed by biochemical tests and by PM-PCR and pathogenicity was established in Swiss albino mice before vaccine production. The LD50 (11 w) of the DP1 isolate determined was 10 CFU / bird and MDT was 23.75 h in 11 week old ducklings when a high dose of 3 X 10 6 CFU of P. multocida per bird was given. At 11 Weeks age MDT gradually increased as the dose of inoculum decreased. In 21 week old ducks, the LD50 (21w) of the isolate DP1 was estimated to be 3 ×108 CFU of P. multocida and it showed that the Kuttanad duck had decreased susceptibility to pasteurellosis with age. On light microscopic studies planktonic cells appeared to be Gram negative coccobacillary, while biofilm cells were Gram negative and pleomorphic. Electron microscopic studies revealed that P. multocida could form well differentiated classic biofilm and 0.32 per cent TSB media supplemented 0.5 per cent chitin seemed to be excellent medium for biofilm formation by P. multocida. Four different vaccines viz. OV, OBV, SV and SAV were prepared and all of them were found to be sterile and safe. The oil adjuvanted vaccines (OV and OBV) offered better protection compared to saponin and aluminium hydroxide adjuvanted vaccine groups (SV and SAV) following primary vaccination, up to seven weeks. The modified oil adjuvanted vaccine prepared was not only found to be homogenous, but also more efficient in stimulating a humoral immune response and hence may be recommended. The oil adjuvanation gave better protection than saponin and aluminium hydroxide adjuvanation. The SAV gave better protection than SV which might be due to the presence of aluminium hydroxide which potentiated the immunostimulating ability of saponin. The combined vaccine (SAV) although was found to be better than single vaccine (SV) they cannot be used as a substitute to oil adjuvanted vaccines. The booster vaccination was found to have added advantageous effect on protection and is a must, to prevent losses. Pasteurella biofilms although found to be weak in inducing a primary immune response had the potency to evoke a more powerful secondary response compared to planktonic cells. Vaccination done at six weeks age followed by booster vaccination at 16 weeks age seemed to be a better modification of existing schedule and may be recommended. In histopathological studies, lymphoid hyperplasia was observed in spleen in survived control birds and in SV and SAV vaccine groups that did not survive challenge test, which indicated the persistence of Pasteurella organisms through mild infection in them following experimental challenge. Lymphoid depletion was observed in caecal tonsil in experimental pasteurellosis as in spleen. As the survived vaccinated birds following challenge test showed normal intact caecal tonsil, the course of disease and lesions might be less prominent in vaccinated birds during infection process. The well developed bursa observed in OV and OBV birds that survived challenge test indicated that the humoral immune response was well induced in them compared to other groups. The designed primers E1 and E2 amplified the gene E and hence, this pair of primers could be used for the production of amplified Gene E sequences for further studies on recombinant ghost system. In conclusion, 0.32 per cent TSB media supplemented 0.5 per cent chitin seemed to be an excellent medium that support classical biofilm formation by P. multocida. Booster vaccination definitely had added advantageous effect on protection. Immunization at 6 weeks of age with OV followed by booster vaccination at 16 weeks age with OBV seemed to be a better modification of existing schedule and may be recommended. In histopathological studies, the lesions were less prominent in vaccinated birds than control birds which indicated that the vaccines were effective.ThesisItem Open Access Evaluation of coffee husk for milk production in cows(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1979) Geevarghese, P I; KAU; Subramaniam, MAn investigation was carried out to find out the feeding value of coffee husk for milk production in cows using a total of nine Sindhi x Jersey cross bred cows, divided into three groups of three animals each. The experiment was for a period of 90 days. Coffee husk was included in the concentrate mixture at 0, 10 and 20 per cent levels. A switch – over design was used for the experiment. Coffee husk fed at 10 and 20 per cent levels in the concentrate ration did not significantly influence the body weight of animals. The total milk production of the animals getting coffee husk in the ration did not significantly differ from that of the animals on the control diet. The percentage of fat in milk, the total quantity of milk fat produced, the amount of four per cent fat – corrected milk, the percentage of total solids, the amount of total solids in milk, percentage of solids – not –fat, total quantity of solids – not – fat and the amount of solids – corrected milk remained the same for all the three groups of animals included for the study and no significant differences were noticed due to treatments. The physiological status of the cows in all the groups was normal and satisfactory. No significant difference due to treatments was noticed in some of the physical and chemical constants of butter fat. The dairy merit (percentage) based on efficiency of feed conversion was less of animals getting ten per cent coffee husk due to the reduced milk production and the greater feed consumption. The total cost of feed for producing one kg milk was Rs.1.42, 1.38 and 1.33 for animals getting 0, 10 and 20 per cent coffee husk in the concentrate mixture respectively. It was concluded that coffee husk upto 20 per cent level can profitably be incorporated in the concentrate mixture of dairy cows.ThesisItem Open Access Study of the calf starter with locally available feed ingredients(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1978) Francis, U T; KAU; Subramanyam, MAn investigation was carried out to compare the physiological status and performance of the calves fed two different kinds of calf starters. The calves fed with whole milk and concentrates were used as the control. A total of 18 crossbred calves of the University Livestock Farm, Mannuthy, immediately after birth were assigned at random to one of the following three groups. Group I (control), group II (fed with calf starter I) and group III (fed with calf starter II). There were one male and five female calves in each group. The two calf starters used for the experiment contained 24 per cent protein and 68 per cent TDN. The calves on the experimental group started getting calf starter on the eighth day onwards and the milk was completely withdrawn at the beginning of the fifth week of age. The calves of the control group were fed with concentrates at the beginning of the fifth week when the quantity of the milk was reduced. At 12 weeks of age milk was completely withdrawn. The feeding trial was a period of 24 weeks from the birth of the calves. The statistical analyses of the data collected revealed that the growth rate of calves fed calf starter I was significantly higher as compared to the calves fed calf starter II. But the growth rate of calves in Groups I and II was almost the same. The total gain in body weight was 44.17 kg in a period of 24 weeks for the calves getting calf starter I as compared to the value of 43.08 and 30.91 for groups I and III respectively. Eventhough there was no significant difference with regard to other body measurements in the three groups, the calves that received calf starter I had a higher paunch girth in comparison to the calves on calf starter II. The physiological status of the calves in all the groups as revealed by the study of the blood value was normal and satisfactory. Eventhough all the calves showed a positive nitrogen balance at the termination of the experiment the valves fed calf starter I had a greater nitrogen balance. Calf starter I was found to be beneficial in terms of general condition, physiological status and weight gain of the calves. By incorporating calf starter I in the feeding schedule of calves, a quantity of 141.4 kg whole milk could be made available for human consumption in addition to a saving of Rs. 186.83 in the cost of feeding a calf during the first 24 weeks of age.
