Thumbnail Image

Kerala Agricultural University, Thrissur

The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively. Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively. On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs. In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc. During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU). Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.

Browse

20071
Reset filters
Subject: Crop Physiology × Author: KAU × Author: Lissamma, Joseph × End date: 2016 × Type: Thesis × Thesis Type: Ph.D ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Genetic transformation of black pepper (piper nigrum L) for phytophthora foot rot resistance/tolerance
    (Department of plantation crops and spices, College of horticulture, Vellanikkara, 2007) Lissamma, Joseph; KAU; Valsala, P A
    Investigations on "Genetic transformation of black pepper (Piper nigrum L.) for Phytophthora foot rot resistance/tolerance" were carried out at the Department of Plantation Crops and Spices, and Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara during 2001-2006. Three selected black pepper varieties Panniyur 1, 4 and 6 were utilized for the study. Axenic cultures of the selected varieties were raised from nodal segments as well as ripe seeds for embryogenesis and transformation studies. Among the varieties average number of multiple shoot/explant was high for variety P­6 in ½ MS medium with BA and IAA 1.0 mg l-1. Somatic embryogenesis was induced on ripe seeds of the variety P6 at the micropylar region in ½ MS medium with inositol 1.0 g l-1. Further development of the somatic embryo observed in liquid shaking cultures of SH basal. Growth regulators like BA,2,4-D ,dicamba and thidiazuron did not give rise to embryogenesis in the different explants of black pepper. Multiple shoot induction from cotyledonary nodal explants and zygotic embryo explants were observed in all the varieties in ½ MS with 1.0 mg l-1 BA and IAA. Direct regeneration from leaf segments was also observed in the same media combination. Agrobacterium tumefaciens strains EHA105, AGL.1.1303, GV2260 and LBA4404 were used for the transformation work. Strain EHA105 contains the plasmid p35SGUSINT with gus A gene and npt II gene. The AGL.1.1303 contains the plasmid harbouring antibiotic resistant selectable marker genes (npt II and hpt IV) and GUS and GFP reporter genes. The GV2260 contain the plasmid pGV2260 with osmotin gene and npt II gene. The LBA4404 contains the plasmid pBZ100 containing alfalfa glucanase gene, rice chitinase gene and npt II gene. Sensitivity studies of black pepper tissues to various antibiotics resulted in selecting the optimum threshold level of antibiotic to be used in the screening medium. Kanamycin 25 mg l-1, 50 mg l-1, and 100 mg l- were selected as the cut off level for the selection of transformants from zygotic embryo, leaf segments and nodal segments respectively. Cefotaxime at 250 mg l-1 was selected for the effective elimination of Agrobacterium after infection. Genetic transformation was standardized with Agrobacterium strain EHA105 using leaf disc and zygotic embryo explants. Tentative protocol for transformation in black pepper include Agrobacterium inoculum density 0.9, infection time 10 min and co-cultivation period of 48 h. Acetosyringone at 50-100 µM favoured transformation. Transient gus assay revealed faint blue staining on the infected leaf explants. Explants, leaf segment, cotyledonary node and zygotic embryo were used for transformation with Agrobacterium strains AGL.1.1303, GV2260 and LBA4404. There was explant specificity for the different Agrobacterium strains used. With LBA4404 zygotic embryo explants gave maximum survival in the screening medium containing 50 mg l-1 kanamycin and 250 mg-1 cefotaxime. Direct gene transfer using gene gun attempted with pBZ100 and cotyledonary node explants. Bombarded explants were found surviving in the screening medium with kanamycin for four months. However molecular analysis of selected transformants through PCR revealed that npt II gene integration has not happened in the tissues subjected to PCR analysis.