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Acharya N G Ranga Agricultural University, Guntur

The Andhra Pradesh Agricultural University (APAU) was established on 12th June 1964 at Hyderabad. The University was formally inaugurated on 20th March 1965 by Late Shri. Lal Bahadur Shastri, the then Hon`ble Prime Minister of India. Another significant milestone was the inauguration of the building programme of the university by Late Smt. Indira Gandhi,the then Hon`ble Prime Minister of India on 23rd June 1966. The University was renamed as Acharya N. G. Ranga Agricultural University on 7th November 1996 in honour and memory of an outstanding parliamentarian Acharya Nayukulu Gogineni Ranga, who rendered remarkable selfless service for the cause of farmers and is regarded as an outstanding educationist, kisan leader and freedom fighter. HISTORICAL MILESTONE Acharya N. G. Ranga Agricultural University (ANGRAU) was established under the name of Andhra Pradesh Agricultural University (APAU) on the 12th of June 1964 through the APAU Act 1963. Later, it was renamed as Acharya N. G. Ranga Agricultural University on the 7th of November, 1996 in honour and memory of the noted Parliamentarian and Kisan Leader, Acharya N. G. Ranga. At the verge of completion of Golden Jubilee Year of the ANGRAU, it has given birth to a new State Agricultural University namely Prof. Jayashankar Telangana State Agricultural University with the bifurcation of the state of Andhra Pradesh as per the Andhra Pradesh Reorganization Act 2014. The ANGRAU at LAM, Guntur is serving the students and the farmers of 13 districts of new State of Andhra Pradesh with renewed interest and dedication. Genesis of ANGRAU in service of the farmers 1926: The Royal Commission emphasized the need for a strong research base for agricultural development in the country... 1949: The Radhakrishnan Commission (1949) on University Education led to the establishment of Rural Universities for the overall development of agriculture and rural life in the country... 1955: First Joint Indo-American Team studied the status and future needs of agricultural education in the country... 1960: Second Joint Indo-American Team (1960) headed by Dr. M. S. Randhawa, the then Vice-President of Indian Council of Agricultural Research recommended specifically the establishment of Farm Universities and spelt out the basic objectives of these Universities as Institutional Autonomy, inclusion of Agriculture, Veterinary / Animal Husbandry and Home Science, Integration of Teaching, Research and Extension... 1963: The Andhra Pradesh Agricultural University (APAU) Act enacted... June 12th 1964: Andhra Pradesh Agricultural University (APAU) was established at Hyderabad with Shri. O. Pulla Reddi, I.C.S. (Retired) was the first founder Vice-Chancellor of the University... June 1964: Re-affilitation of Colleges of Agriculture and Veterinary Science, Hyderabad (estt. in 1961, affiliated to Osmania University), Agricultural College, Bapatla (estt. in 1945, affiliated to Andhra University), Sri Venkateswara Agricultural College, Tirupati and Andhra Veterinary College, Tirupati (estt. in 1961, affiliated to Sri Venkateswara University)... 20th March 1965: Formal inauguration of APAU by Late Shri. Lal Bahadur Shastri, the then Hon`ble Prime Minister of India... 1964-66: The report of the Second National Education Commission headed by Dr. D.S. Kothari, Chairman of the University Grants Commission stressed the need for establishing at least one Agricultural University in each Indian State... 23, June 1966: Inauguration of the Administrative building of the university by Late Smt. Indira Gandhi, the then Hon`ble Prime Minister of India... July, 1966: Transfer of 41 Agricultural Research Stations, functioning under the Department of Agriculture... May, 1967: Transfer of Four Research Stations of the Animal Husbandry Department... 7th November 1996: Renaming of University as Acharya N. G. Ranga Agricultural University in honour and memory of an outstanding parliamentarian Acharya Nayukulu Gogineni Ranga... 15th July 2005: Establishment of Sri Venkateswara Veterinary University (SVVU) bifurcating ANGRAU by Act 18 of 2005... 26th June 2007: Establishment of Andhra Pradesh Horticultural University (APHU) bifurcating ANGRAU by the Act 30 of 2007... 2nd June 2014 As per the Andhra Pradesh Reorganization Act 2014, ANGRAU is now... serving the students and the farmers of 13 districts of new State of Andhra Pradesh with renewed interest and dedication...

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2016 - 201912020 - 20211
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Subject: Plant Pathology × Author: CHIRANJEEVI, N × Start date: 2000 ×
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    ThesisItemOpen Access
    INVESTIGATIONS INTO ANTAGONISTIC BASIS OF ROOT ENDOPHYTIC BACTERIA IN THE CONTROL OF CHICKPEA DRY ROOT ROT PATHOGEN (Rhizoctonia bataticola (Taub.) Butler)
    (ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, GUNTUR, 2021-12-21) CHIRANJEEVI, N; REDDI KUMAR, M
    A survey was conducted during January, 2017 Rabi season in five major chickpea growing districts of Andhra Pradesh viz., Kurnool, Y.S.R Kadapa, Anantapuramu, Prakasam and Guntur to assess the status of dry root rot incidence under field conditions. Mean maximum dry root rot incidence was observed in Anantapuramu (11.50 %) followed by Kurnool (9.61 %), Y.S.R Kadapa (7.92 %) and Prakasam (7.50 %) and the least was observed in Guntur (6.00 %). Disease occurrence was observed irrespective of cropping system, soil types and cultivars. The disease incidence was low in the irrigated fields compared to rainfed fields. A total of 22 isolates of R. bataticola were obtained and purified by single sclerotial isolation technique. Variability in the cultural and morphological characters of 22 isolates of R. bataticola were studied by growing on potato dextrose agar medium. Among all the isolates CRb 10 taken significantly least time (2.50 days) to occupy the full plate and higher radial growth at 42 hrs was observed with CRb 10 isolate (75.78 mm). However, fluffy colonies were produced by CRb 2 and CRb 15 isolates while, velvety colony texture was observed with CRb 13 and remaining all the isolates showed appressed colony texture. Similarly, out of 22, jet black, light black and grey colour mycelia was observed in total of seventeen, four and one isolates respectively. Among all the isolates, significantly maximum time was taken by CRb 18 isolate for sclerotial initiation and least time was taken by CRb 16 (1.83 days). However, among the isolates higher number of sclerotia per 10 x microscopic field and sclerotial size was observed with CRb 16 (33.33) and CRb 6 (119.32 µm) respectively. Similarly, among 22 isolates, irregular, ovoid and round shaped sclerotia were observed in total of 3, 9 and 10 isolates respectively. Pathogenicity of R. bataticola isolates was proved by soil inoculation method. When observations were collected on disease incidence (%), among the isolates tested maximum disease incidence (100 %) was observed in CRb 9 and it was considered as a most virulent pathogen isolate and used for further studies. A total of 40 antagonistic endophytic bacteria were isolated from healthy roots of different chickpea varieties and the antagonistic efficiency was tested using dual culture technique. Among the isolates CREB 37 showed maximum antifungal efficiency with 74.04 % inhibition against virulent R. bataticola isolate CRb 9. Based on the results it was considered as a potential isolate and used for further studies. Mannitol @ 2 %, soytone @ 2 % pH 7 and temperature 30oC were found to be optimum for cell growth and antibiotic production. Similarly, significant improvement in the cell growth and antibiotic production was observed with the modified Luria broth than the basal medium (Luria broth). Crude antibiotic substances were extracted and tested their bio-efficacy against R. bataticola by agar well diffusion assay. Among the different concentrations tested significantly higher inhibition was recorded at 100 µl (77.23 %). The microscopical examination of the crude antibiotic extract treated mycelia of the R. bataticola revealed its effect on the fungal morphology. Light microscopic analysis revealed that severe mycelial destruction and the SEM images clearly explained the disruption of the fungal hyphae due to the mechanistic effect of antibiotic compounds on fungal cell wall. Among the 14 potential isolates, Gram negative (7) and Gram positive (7) were recorded in equal proportion and all were rod shaped bacteria. Out of the 14 isolates, a total of 9, 7, 2, 1, 2, 3, 3, 13, 6, 3 and 0 showed positive for amylolytic activity (starch hydrolysis), proteolytic activity (Gelatin hydrolysis), lactose utilization, indole production, methyl red test, Voges-Proskeur test, citrate utilization, presence of catalase activity, ammonia production, HCN production and siderophore production respectively. 16S r DNA sequence analysis was used to identify the potential bacterial isolates such as CREB 9, CREB 21 and CREB 37 and which were exhibited close similarity of 97.75, 98.48 and 93.28 per cent with Enterobacter cloacae, Pseudomonas aeruginosa and Bacillus subtilis respectively. Talc based formulation of potential endophytic bacterial isolate B. subtilis (CREB 37) was prepared and tested the bio-efficacy of formulation and crude antibiotic extract in pot culture. Under glasshouse conditions treatment T7 (seed treatment with potential bacterial antagonist (talc formulation) @ 10 g kg-1 + soil application with talc based formulation of potential bacterial antagonist @ 100 g pot-1) was found to be superior as it recorded maximum germination per cent, plant height, root length, dry weight and least per cent disease incidence compared to other treatments.
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    ThesisItemOpen Access
    “MICROBIAL SYNTHESIS AND CHARACTERIZATION OF NANO SCALE SILVER MATERIAL AND EVALUATION OF THEIR BIO EFFICACY AGAINST RICE SHEATH BLIGHT PATHOGEN Rhizoctonia solani (Kuhn)
    (Acharya N.G. Ranga Agricultural University, Guntur, 2016) CHIRANJEEVI, N; ANIL KUMAR, P
    In detached leaf technique inoculation with two days old culture disc of R. solani initiated sheath blight disease of rice swiftly than sclerotial body inoculation. In rolled towel paper method, with seed inoculation of R. solani, 16.7% germination, 38.9% shoot length, 48.2% root length and 52.7% vigour index was reduced. Two of the eight Pseudomonas fluorescens isolates tested in dual culture against R. solani, viz., PF-2 and PF-5 were found potentially antagonistic in vitro sustaining inhibitory effect. Two of the six Trichoderma isolates viz., ET-1 and RT-4 were found potentially antagonistic with complete overgrowth on R. solani under in vitro. All the four test antagonistic isolates and the pathogen could convert silver in to bionano silver with absorbance peaks around 400 to 450 nm. Exponential growth of the organism was found efficient in nano conversion, especially in case of P. fluorescens. Cellulose among the five carbon sources and casein among the five nitrogen sources used as nutrients were found to support better in nanoconversion by Trichoderma ET-1. Ethyl acetate and Diethyl ether extracts of ET-1 culture filtrate were found to contain nanoconversion principle with 0.0298% and 0.0154% as dilution end points respectively. Zeta potential of P. fluorescens PF-2 based bionano silver particles prepared from 5 day old culture filtrate was -34.4 mV, R. solani based nano particles prepared from 5 day old culture filtrate was -36.0 mV and Trichoderma ET-1 based nano particles from 5 day old culture filtrate was -0.3mV indicating rapid coagulation and flocculation property of ET-1 based nano preparation. Dynamic light Scattering spectroscopy revealed that bionano silver particle size ranged from 80 to 137.4nm (98.2±28.7nm) with PF-2 based nano and 98.8 to 223.7nm (160.9±62.1nm) with R. solani based nano indicating more of polydisperse in nature. In case of bionano silver prepared from ET-1 culture filtrate, the particle size ranged from 6.3 to 6.9nm (6.7±0.3nm) indicating its monodisperse nature. Results of SEM analysis was also in line with DLS analysis. Bionano preparations, irrespective of the isolate source used, were found effective in decreasing the sheath blight incidence in vitro though variation existed in terms of quantum of disease reduction. Satisfactory reduction in disease development at 100% and 50% concentrations of bionano silver was obtained compared to 10%. Bionano preparations were effective against all the three selected test isolates of R. solani, Trichoderma and P. fluorescens though the inhibition was much pronounced on bacterial culture. Increased germination of rice seeds, shoot length, root length, S:R ratio, fresh weight, dry weight and vigour index of rice seedlings with 10% silver bionano preparations applied as seed treatment (wet seed treatment for 10 minutes) was observed. Plant defense related enzymes viz., peroxidases, polyphenol oxidase, phenyl alanine ammonia lyase and tyrosinase were substantially accumulated in treated rice seedlings indicating induction of resistance mechanism in rice seedlings.