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Acharya Narendra Deva University of Agriculture & Technology, Ayodhya
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ThesisItem Open Access Studies on genetic variability for morpho-economical traits in tomato (Solanum lycopersicon (Mill.) Wettsd.)(ANDUAT, Kumarganj, Ayodhya, 2016-07-05) Bajpai, Rohit Kumar; Yadav, G.C.The present investigation entitled “Studies on genetic variability for morpho-economical traits in tomato (Solanum lycopersicon (Mill.) Wettsd.)” was executed at Main Experiment Station, Department of Vegetable Science, Narendra Deva University of Agriculture and Technology, Narendra Nagar (Kumarganj), Faizabad. during Rabi, 2014-2015, to assess variability, heritability and genetic advance for quantitative characters, to estimate correlation coefficients among the important economic traits, to find out the direct and indirect effect of yield components on yield by path coefficient analysis and to judge the genetic divergence in the available genotypes of tomato. Experimental material for the study consisted of 46 genotypes including 3 checks (DVRT-2, H-24, H-86). The experiment was conducted in Randomized Complete Block Design with three replications. Each treatments consisted of 20 plants in two row, having spacing of 60 cm X 45 cm. Observations were recorded on thirteen quantitative characters viz. days to 50 per cent flowering, plant height (cm.), primary branches per plant, number of fruits per plant, days to first fruit harvest, average fruit weight (g.), fruit circumference (cm), pericarp thickness (cm), number of locules per plant, TSS (%), fruit length (cm), harvesting duration and fruit yield per plant (kg). The analysis of variance revealed that the mean sum of squares due to treatments was found highly significant for all the characters. The higher magnitude of coefficient of variation at phenotypic as well as genotypic levels was observed for number of locules per fruit and plant height (cm). While low GCV and PCV were observed for days to 50 per cent flowering and days to first fruit harvest. High heritability coupled with high genetic advance in per cent of mean was recorded for number of fruits per plant, plant height, primary branches per plant, fruit circumference and number of locules per fruit indicating that these traits were less influenced by environment. The most important trait yield per plant had exhibited highly significant and positive phenotypic correlation with number of fruits per plant, plant height (cm), primary branches per plant and fruit length (cm). The highest positive direct effect on fruit yield per plant was exerted by number of fruit per plant followed by average fruit weight (g), pericarp thickness (cm), plant height (cm), number of locules per fruit, fruit circumference (cm) and harvest duration (days).ThesisItem Open Access Tissue culture of Grand Naine banana (Musa paradisiacal L.) And its evaluation in the field(ANDUAT, Kumarganj, Ayodhya, 2016-07-11) Singh, Avinash kumar; Singh, K.N.Banana is the most popular fresh fruit all over the world and its name comes from the Arabic word 'banan', which means finger. The scientific name of banana is Musa acuminate and Musa balbisiana. But the old scientific names of banana are Musa sapientum and Musa paradisiacal L. Bananas are rich source of carbohydrates and potassium. The present study reports a very simple one step protocol using MS with BAP for initiation, multiplication and elongation of shoot and MS with IAA for rooting of banana. The protocol raised in the present attempt could be used for the massive in vitro production of the plantlets of the banana. In the beginning, Sucker was taken as explant and inoculated in sterilized MS media.Theblackening of rhizomes during sterilization and culture were observed. The pretreatment of explants with bavistin 1gm carbendazim systemic fungicides 1gm and almox 10 mg used for 5 suckers treatment for a period of one hour before sterilization with thorough washing in running tap water and surface sterilization. Rhizome were cut into small size and treated with different reagents.Shoot initiations were observed after 3-5 days of inoculation. Single leaf development in shoot was observed in media containing 2mg/l BAP. In media containing 3.0 and 4.0mg/l BAP sprouting were observed better. Increase in concentration of BAP to 5.0mg/l along with 0.1mg/l of kinetin and IAA were growth inhibitory to number of shoot, number of leaves and length of leaves.The root initiation was activated on basal medium without any growth regulators.Rooting was stimulated when shoots were transferred to basal medium of 0.1mg/l IAA, Kinetin and activated charcoal.Rooting and shooting elongation was initiated after 20-30 days. Hardening was successfully carried out in in-vitro raised plantlets by transferring the plants in hormone free medium for period of 20 days under controlled environment condition. The tissue culture raised plantlets were transferred to soil in pre autoclaved soil mixture.Flowering started 9 to 10 months after planting. 130 fingers\bunches are found in banana cv. Grand Naine.ThesisItem Open Access Growth models for sugarcane production in uttar pradsh(ANDUAT, Kumaraganj, Ayodhya, 2013-07-06) Rao, Vishwajeet Pratap; Sisodia, Dr. B.V.S.The present investigation entitled “growth models for sugarcane production in Uttar Pradesh.” consist five chapters including summary and conclusion. The purpose of the study is to find trend and growth rate of sugarcane area, production and productivity in different three last decades in Uttar Pradesh. The Chapter –I, introduces the subject of investigation. The main objectives are also outlined in this chapter. The review of relevant literature is presented in Chapter-II. The material and methods are described in Chapter-III. The time series data on sugarcane area, production and productivity in Uttar Pradesh, pertaining to periods 1980-81 to 2009-10, were used for investigation of trend and growth rate of sugarcane area, production and productivity. The relevant statistical tools & technology like regression analysis etc. have been used for the purpose of investigation mentioned above. The results are presented in chapter-IV. On the overall, the results show that the growth rate of sugarcane area, production and productivity has increased during all the decades and during the entire period of the study.ThesisItem Open Access Comparative studies of susceptible and tolerance varieties of pigeon pea (Cajanus cajan) using protein profiling and RAPD markers with response to Pod borer (Helicoverpa armigera) resistance(ANDUAT, Kumarganj, Ayodhya, 2016-07-11) Maurya, Rajesh Kumar; Khan, N.A.Pigeonpea seeds are important source of proteins, energy and other nutrients in the diets of large population groups around the world, forming and excellent source of lysine, methionine and tryptophan and other water soluble vitamins (riboflavin, niacin and folacin) and of the minerals phosphorus, iron and magnesium (Ramcharan and Walker, 1985). The present study involved evaluation of ten genotypes of pigeonpea including five susceptible and five resistant varieties viz., NDA-1, NDA-2, Bahar, NDA-14-6, NDA-13-6, NDA-3, UPAS-120, MAL-6, MAL-13, Cajanus scarabaeoides (wild). The work was done with 10 %, 12 %, 15 % protein profiling, 12 % native gel analysis, DNA isolation and RAPD analysis. The result obtained on above characters was analyzed for protein profiling and RAPD analysis. Gel electrophoresis of protein of pigeonpea varieties show novel band obtain at the upper portion of the 10 % acrylamide gel , these band only seen 0.455 (RM value) is pod borer tolerant and regarded as pod borer resistant responsible protein. RAPD primer was initially tested for the PCR amplification of the genomic DNA for 10varieties, but they did not amplify genomic DNA. Genomic DNA was amplified with RAPD specific primer i.e OPA-15, OPU-11 and OPA-16, but no result was found. DNA and RAPD analysis was the best tools for identification of resistant and susceptible gene for podborer (Helicoverpa armigera).ThesisItem Open Access Efficiency of selection based on genetic variability, path coefficient and genetic divergence in ridge gourd[Luffa acutangula L.(Roxb.)](ANDUAT, Kumarganj, Ayodhya, 2016-06-16) Bhargava, Amit Kumar; Singh, V.B.The experiment of the present research work entitled “Efficiency of selection based on genetic variability, path coefficient and genetic divergence in ridge gourd [Luffa acutangula L. (Roxb.)]” was conducted during zaid 2015. In this experiment twenty nine germplasm were planted on 20th March, 2015 at Main Experiment Station, Department of Vegetable Science, at Narendra Deva University of Agriculture & Technology, Narendra Nagar (Kumarganj), Faizabad (U.P.). The crop continued till 16th July 2015. The experiment was conducted in Randomized Block Design with three replications. The material used in the experiment comprised of twenty two selected variable and pure germplasm of ridge gourd, including Pusa Nasdar a national check. The experimental data was compiled by taking the mean value of the 29 genotypes of ridge gourd for 13 yield and its components traits from all the three replications. Then it was subjected to the following statistical analyses; to evaluate mean performance, genetic variability, heritability, genetic advance, correlation as well as path coefficient and genetic divergence for yield and its components traits. The results of present studies indicate top five varieties showed highest marketable fruit yield per plant was NDRG-9 followed by NDRG-5, NDRG-21, NDRG-1, NDRG-26. Besides, these germplasm also possessed average to high mean performance for most of the yield components. The data recorded on thirteen quantitative characters were subjected to analysis of variance to test the significance of difference among the genotypes. Analysis of variance showed that the mean square due to genotypes were highly significant for all the thirteen characters. The higher magnitude of coefficient of variation at genotypic as well as phenotypic levels were observed higher for node number to anthesis of first staminate flower followed by node number to anthesis first pistillate, marketable fruit yield per plant and average fruit diameter while days to first fruit harvest had lowest coefficients of variation. The presences of high heritability in broad sense were observed for character except node number to anthesis of first staminate flower. Moderate heritability and high genetic advance in per cent of mean was recorded for number of nodes per vine followed by average fruit length and marketable fruit yield per plant showing additive gene affect and very good response to selection for improving above yield and yield contributing traits. Number of fruits per plant showed highly significant positive correlation with marketable fruit yield per plant (kg) and days to first fruit harvest. Path coefficient analysis revealed days to first fruit harvest indicated highest positive direct effect on marketable fruit yield per plant followed by node number to anthesis of first staminate flower and number of fruit per plant. Twenty nine genotypes were grouped into 6 non-overlapping clusters. The different clusters showed considerable differences in intra-cluster group means for all the traits. Therefore, crosses between members of clusters having high cluster means for important characters coupled with high inter-cluster distances between them, are likely to be more useful. Major cluster III and VI in divergence analysis contained genotypes of heterogeneous origin, thereby, indicating no parallelism between genetic and geographic diversity. Therefore, crosses between members of clusters separated by high inter-cluster distance are likely to produces desirable heterotic F1(s) and segregates. The maximum intra-cluster distance was observed in case of cluster III followed by cluster II and the maximum inter-cluster distance was found between cluster IV to cluster VI followed by cluster IV to cluster V and cluster III to cluster VI. Genotypes of these diverse clusters can be used for producing heterotic F1 and desirable segregates.ThesisItem Open Access Studies on genetic variability analysis in rice (Oryza sativa L.) under saline-alkaline condition(ANDUAT, Kumarganj, Ayodhya, 2015-06-24) Chaudhary, Chritra; Verma, G.P.The present investigation entitled “Studies on genetic variability analysis in rice (Oryza sativa L.) under saline- alkaline condition” was conducted at Genetics and Plant Breeding Research Farm, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad under saline-alkaline soil condition (8.9 pH=8.9; EC=2.45dSm-1; ESP=44%) during Kharif 2014. The experimental materials consisted of eighty germplasm and four checks viz., CSR 10, Jaya, Narendra Usar 3 and Swarna Sub 1. These genotypes exhibited wide spectrum of variability for various agronomical and morphological characters. The experiment was conducted in Augmented Block Design having eight blocks of fourteen plots in each block. Each plot consisted of single row of 3 meter length with the spacing between rows and within plants 20 cm and 15 cm, respectively. The characters studied were days to 50% flowering, flag leaf area (cm2), plant height (cm), panicle bearing tillers per plant, panicle length (cm), spikelets per panicle, spikelet fertility (%), 1000-grain weight (g), grains per panicle, L/B ratio, biological yield per plant (g), harvest index (%) and grain yield per plant (g). The data recorded on these characters was utilized for estimation of mean, range, variance, correlations, path-coefficients and genetic divergence. The salient findings of the study and conclusion drawn are summarized below: The most promising lines showing highest seed yield with high mean performance for yield contributing traits were identified. 1. The genotype NDRK 5064, IR 83439-4-B-11-3-2-1-AJY1-3-B, NDRK 5014, NDRK 5075, A 69-1, NDRK 5008, NDRK 5019 and NDRK 5005 produced high grain yield per plan; IR 45427-2B-2-2B-1-1, NDR 507 and NDRK 50039 for early days to 50 per cent flowering; Narendra 6029, IR 45427-2B-2-2B-1-1 and NDRK 50039 for greater flag leaf area; NDR 507, IR 85920-1-1-2-1-AJY1-2B and FL 478 for short stature; NDRK 50047, NDRK 5059 and NDRK 5064for highest panicle bearing tillers per plant; NDRK 50039, NDRK 5095 and NDRK 5067 for longer panicle length (cm); Vikas, NDRK 5064 and NDRK 5014 for spikelets per panicle; NDRK 5082, NDRK 5015 and NDRK 50047 for spikelet fertility (%); IR 83439-4-B-11-3-2-1-AJY1-3-B, NDRK 5095 and CSR 36for biological yield per plant (g); NDRK 5088, IR 76397-28-6-1-1-1-1 and NDRK 5019for harvest index (%); NDRK 5017,ThesisItem Open Access Studies on genetic veriability and character association for yield components and seed quality parameters in rice (Oryza sativa L.)(ANDUAT, Kumarganj, Ayodhya, 2017-06-28) Singh, Rishabh Kumar; Yadav, H.C.The present investigation was carried out at the Crop Research Station (CRS) Masodha, Faizabad, farm and seed quality parameters were tested in Seed Testing Laboratory of the Seed Technology Section, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad (U.P.). In the present study the experimental material constituted 30 germplasm line with 3 check varieties viz., Shusk samrat, Narendra 359, Narendra 2064. The experiment was laid out in Randomized Block Design (RBD) with three replications in normal soil under irrigated condition during Kharif 2016. Each entry was grown in 5 meter long single row plots with spacing 20 cm and 15 cm within row to row and plant to plant, respectively. The characters studie namely Days to 50% flowering, Days for maturity, Plant height (cm), Panicle length (cm), Seeds/panicle, 1000-seed weight (g), Seed yield/plant (g), Seed germination (%), Seedling dry weight (mg), Germination Index, Vigour index. The high magnitude of genotypic and phenotypic coefficients of variation were observed for seed per panicle, seed yield per plant, vigour index, plant height, indicating thereby, substantial scope for improving in this character after hybridization and subsequent selection the high estimate of heritability with high genetic advance in per cent of mean were observed for seed yield per plant, seed per panicle, vigour index and plant height. The highest seed yield per plant was produced by NDR-359 (28.49 g), while the lowest seed yield per plant were given by Jaya (12.18 g). The general mean calculated for this character was 18.02 g. Ten genotypes, namely, NDR-359, NDR-2024, NDR-2032, Shushk samrat, NDR-2031, Usar-2, NDR-2064, IET-16057, NDR-1087-10 and NDR-1018 were significantly produced seed yield per plant. The most desirable genotypes for characters other than seed yield were Heera, Gajgaur gonad, Karahani, Akatahawa, Dehula-1, NDR-1087-10, Tinpakhiya, NDR-1018, Sapana and Shushk samrat for early flowering; Heera, Tinpakhiya, Gajgaur gonad, Karahani, NDR-1087-10, NDR-1018, Dehula-1, Shushk samrat, Duddhi and Sapana for early maturity; Heera, IET-16068, Pant dhan-11, IET-16062, NDR-1087-10, Tinpakhiya, IET-16057, Gajgaur gonad, NDR-2064 and Jaya for dwarf plant height;ThesisItem Open Access Study on adoption of bt. Cotton in warangal block of warangal District (Telangana State)(ANDUAT, Kumarganj, Ayodhya, 2016-06-28) Kumar, P.Pavan; Doharey, R.K.The study was conducted in Warangal block of Warangal district (Telangana) selected purposively. A total number of 100 respondents were selected through random sampling from 5 sample villages on the basis of majority of Bt. Cotton growers. The structured schedule was developed keeping in view the objectives & variables under study.The respondents were contacted personally for data collection. The percentage, mean, standard deviation, correlation were used for calculation and drawing the inferences. The majority of the respondents 63% were found in middle (37-47) age group, like this 80 % literate, 65% other backward caste, 100% hindu religion, 73% nuclear family, 50% medium family size (5-7 members), 45% small farmers (1 -2 ha), 91% agriculture main occupation, 67% annual income (135000-300000), 70% respondents Participation in one organization, 62% medium overall material possession (31-48 equipment), village development officer I ranked in formal sources, Friends I ranked in informal sources, T. V I ranked in mass media, 57% medium (20-23) economic motivation, 50% medium (22-23) scientific orientation, 68% medium (17-28) risk orientation, 76% medium knowledge and 15% low knowledge and 9% high knowledge on Bt. Cotton cultivation practices, 70% medium, 18% low and 12% high adoption of Bt. Cotton cultivation practices by the respondents. The independent Variables like education, caste, size of land holding, annual income, material possession, economic motivation and extension contact had highly significant and positively correlated with knowledge extent. The independent variables like age, education, caste, size of land holding, annual income, material possession, economic motivation, extension contact had highly significant and positive correlation with adoption of Bt. cotton. The major problems elicited by the respondents were High cost and non- availability of required hybrid seeds at right time, High cost and non - availability of chemical fertilizer in time and more sucking pest attack in Bt. Cotton. The major suggestions offered by the respondents were Provision of improved seed in time with minimum cost, Efforts should be made for providing fertilizers on appropriate rate, Develop sucking pest resistant varieties in Bt. Cotton.ThesisItem Open Access Studies on genetic divergence, character association, path cofficient and genetic variability in newly bred indian musterd [Brassica juncea L. Czern & Coss.](ANDUAT, kumarganj, Ayodhya, 2016-06-30) Charpota, Jayanti Lal; Maurya, K.N.The present investigation entitled “Studies on genetic divergence, character association, path coefficient and genetic variability in newly bred Indian mustard [Brassica juncea L. Czern and Coss.]” was conducted at Genetics & Plant Breeding Research Farm of Narendra Deva University of Agriculture & Technology, Kumarganj, Faizabad (U.P.) during rabi 2014-15. The experimental material constituted 35 germplasm including two check varieties viz. Kranti and NDYR-8.The observations were recorded on eleven different characters namely days to 50% flowering, days to maturity, plant height (cm), primary branches per plant, secondary branches per plant, length of main raceme (cm), siliquae on main raceme, number of seeds per siliqua, seed yield per plant (g), 1000-seed weight (g), oil content (%). The data recorded on these characters were utilized for estimation of mean, range, variance, correlations, path coefficient and genetic divergence. The mean squares due to treatments were highly significant for all the characters under study. The mean squares due to replication were non-significant for all the characters.