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Acharya Narendra Deva University of Agriculture & Technology, Ayodhya

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20161
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Subject: Agricultural Biotechnology × Author: Maurya, Rajesh Kumar ×
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    ThesisItemOpen Access
    Comparative studies of susceptible and tolerance varieties of pigeon pea (Cajanus cajan) using protein profiling and RAPD markers with response to Pod borer (Helicoverpa armigera) resistance
    (ANDUAT, Kumarganj, Ayodhya, 2016-07-11) Maurya, Rajesh Kumar; Khan, N.A.
    Pigeonpea seeds are important source of proteins, energy and other nutrients in the diets of large population groups around the world, forming and excellent source of lysine, methionine and tryptophan and other water soluble vitamins (riboflavin, niacin and folacin) and of the minerals phosphorus, iron and magnesium (Ramcharan and Walker, 1985). The present study involved evaluation of ten genotypes of pigeonpea including five susceptible and five resistant varieties viz., NDA-1, NDA-2, Bahar, NDA-14-6, NDA-13-6, NDA-3, UPAS-120, MAL-6, MAL-13, Cajanus scarabaeoides (wild). The work was done with 10 %, 12 %, 15 % protein profiling, 12 % native gel analysis, DNA isolation and RAPD analysis. The result obtained on above characters was analyzed for protein profiling and RAPD analysis. Gel electrophoresis of protein of pigeonpea varieties show novel band obtain at the upper portion of the 10 % acrylamide gel , these band only seen 0.455 (RM value) is pod borer tolerant and regarded as pod borer resistant responsible protein. RAPD primer was initially tested for the PCR amplification of the genomic DNA for 10varieties, but they did not amplify genomic DNA. Genomic DNA was amplified with RAPD specific primer i.e OPA-15, OPU-11 and OPA-16, but no result was found. DNA and RAPD analysis was the best tools for identification of resistant and susceptible gene for podborer (Helicoverpa armigera).