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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Biotechnology and Molecular Biology × End date: 2009 × Start date: 2009 × Type: Thesis ×
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    ThesisItemOpen Access
    GENETIC TRANSFORMATION STUDIES IN BELL PEPPER (Capsicum annuum L.)
    (2009) VERMA, SWATI; SRIVASTAVA, D.K.
    ABSTRACT The research work was conducted to standardize a protocol for genetic transformation in bell pepper. Plant regeneration studies were carried out using two types of explants viz hypocotyl and cotyledon. The cotyledon explants showed high frequency of shoot regeneration (80.95%) on MS medium supplemented with 6.0 mg/l BA and 0.3 mg/l IAA as compared to hypocotyl (only 8%) on MS medium supplemented with 4.5 mg/l BA and 0.1 mg/l IAA. The shoots regenerated after 25-30 days. The regenerated shoots were in the form of profuse rosette, which elongated to about 2-2.5 cm on MS medium supplemented with 2.25 mg/l BA and 2 mg/l GA3. MS medium containing 0.25 mg/l IAA was found to be best for root regeneration (50%). Regenerated plantlets were acclimatized successfully. During kanamycin sensitivity test, kanamycin concentration as low as 20 mg/l was found to be inhibitory to the growth of bell pepper cells. For genetic transformation, disarmed Agrobacterium tumefaciens LBA 4404 strain containing a reporter -glucuronidase (gus) gene in binary vector (pBI 121) system along with kanamycin resistance gene (npt-II) for selection in both bacteria and plant was used for co-cultivation experiment to transfer gus and npt-II genes in bell pepper cells (cotyledon and hypocotyl explants). After co-cultivation only the transformed cells were able to grow on selective shoot regeneration medium which formed callus and regenerated shoots, whereas non transformed cells/explants died on the selective medium. The elongation of the putative transgenic shoots was achieved on selective elongation medium. Root regeneration in the putative elongated transgenic shoots was obtained on selective root regeneration medium. Out of the total 15 putative transgenic shoots developed, 5 were randomly selected for PCR analysis using designed primers for npt-II and gus genes. Only two shoots/plantlets showed amplified gene separately indicating the presence of npt-II and gus gene(s) in the transgenic shoots/plantlets. Expression of the gus gene was confirmed by GUS assay. The PCR positive shoots/plantlets have shown the expression of gus gene as well. A protocol for genetic transformation in bell pepper has been standardized.