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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    EFFECT OF ADDITIVES IN MEDIUM ON IN-VITRO MATURATION AND FERTILIZATION OF GOAT OOCYTES
    (Assam Agricultural University, Khanapara, Guwahati, 2016-07) BORAH, DHARITRI; Biswas, R. K.
    A total of 2539 oocytes were recovered from 712 goat ovaries obtained from slaughter house soon after sacrifice and the mean recovery rate of oocytes per ovary was 3.43 ± 0.06, 4.21 ± 0.08 and 3.24 ± 0.78 by aspiration, slicing and puncture techniques respectively, being significantly higher (P<0.01) in slicing as compared to other two techniques. The recovery of good quality oocytes with two or more cumulus cells layers around the oocytes was significantly higher (P<0.01) in puncture (73.92 ± 0.92%) than that in aspiration (66.27 ± 0.68%) and slicing (64.76 ± 0.92%) techniques. The effect of addition of 10ng/ml EGF + 50 ng/ml IGF-1, 10ng/ml EGF + 600µM/ml cysteine and 10ng/ml EGF + 0.2mM/ml sodium pyruvate in TCM-199 + 100µl/ml foetal bovine serum + 100µM/ml cysteamine + 1µg/ml 17β-Oestradiol + 5µg/ml pFSH + 5µg/ml oLH + 10 per cent follicular fluid and 10 per cent estrous goat serum (control medium) was studied for in-vitro maturation (IVM) of goat oocytes on incubation at 38.50C for 24 hours in a CO2 incubator maintaining 5 per cent CO2 under humidified condition. The IVM rate of oocytes on the basis of cumulus cell expansion and polar body extrusion was found to be significantly higher (P<0.01) with EGF + IGF-I (88.74± 1.85% and 61.71 ± 1.61%) than that with EGF + sodium pyruvate (82.86 ± 0.97% and 54.62 ± 1.88%), EGF + cysteine (78.58 ± 1.45% and 49.02 ± 1.52%) and control medium (75.27 ± 1.58% and 43.03 ± 1.48%). The oocytes matured in the IVM media used were fertilized in-vitro in Fert-TALP using swimmed-up sperm capacitated in sperm TALP. The incidences of in-vitro fertilization of oocytes on the basis of two polar bodies and 2-cell stage were also higher when oocytes were matured in-vitro using EGF + IGF-I (44.67 ± 8.86 and 15.39 ± 4.48%) than that with EGF + sodium pyruvate (25.51 ± 7.31 and 11.56 ± 4.72%), EGF + cysteine (22.46 ± 8.37 and 11.56 ± 4.72%) and control medium (20.48 ± 4.27 and 8.10 ± 3.84%) although the differences were not found to be significant.