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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20201
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Subject: Veterinary Microbiology × Author: Gogoi, Anamika × End date: 2023 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    CHARACTERIZATION OF OUTER MEMBRANE VESICLES (OMVs) OF Pasteurella multocida OF AVIAN ORIGIN
    (College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2020-01) Gogoi, Anamika; Sharma, R. K.
    The Fowl Cholera, an infectious disease of poultry, waterfowl and many other birds is caused by Pasteurella multocida. To overcome those hurdles in poultry industry, focus has been given to identify immunogenic subcomponent of the causative agent and their use in development of modern vaccines. The present study was undertaken with a view to evaluate immunogenic potential of Outer Membrane Vesicles (OMVs) of Pasteurella multocida as well as their release under the influence of various environmental and physico-chemical factors. The extraction of OMV fraction was made from a highly pathogenic strain of P. multocida capsular type A associated with Fowl Cholera. The release of OMVs by the selected isolates was found to be significantly (p˂0.001) highest under the influence of iron deficient condition (2, 2 bipyridyl), exhibiting a protein concentration of 18.3 mg/ml. Similarly, the influence of pH in iron restricted environment was also have an impact on OMV release, which was found to be significant (p˂0.05) in reverse direction. A positive correlation could also be made in respect to the oxidative and antibiotic stress with release of OMVs. The comparative protein profiling of OMVs, OMPs and whole cell lysate of the selected pathogenic P. multocida type A isolate could exhibit more distinct and prominent protein bands in OMV fraction. The OMV fraction could also reveal the ompA (37.7-38.1 kDa), which was not prominently observed in other two fractions. The immunogenic potential of the extracted OMV fraction revealed an increasing trend of the mean antibody titre in both the immunized groups, with (Group I) or without (Group II) booster. The immunized birds of group I exhibited a significantly rising trend (p<0.05) of the mean serum antibody titre from the day of the vaccination, until it reached its peak (5947.41±62.6). The peak titre was observed on 28th day of post primary immunization, following booster on 21st day post immunization. Similarly, the immunized birds of group II the mean serum antibody titre of 7th dpi was continued to increase significantly at every weeks of observation till it reached peak on 21st (4576.27±42.9). The declining trend of the mean serum antibody titre was observed in the birds of group II from the day 28th of post immunization (4219.12±64.5) and continued till end of the study, i.e. the 60th dpi (3813.83±148.5). No significant difference could be observed between the two preparations, with and without booster in respect to the mean serum antibody titre till 21st dpi. Challenge trial could establish 100 per cent protection of vaccinated birds against homologous challenge, while development of clinical signs in the immunized birds was observed, following heterologous challenge. There was no significant difference between OMVs administered group and control group was observed in terms of blood SOD and GPx activity.