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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20181
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Subject: Plant Pathology × Author: Mishra, Ranima × End date: 2018 × Start date: 2018 ×
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    ThesisItemOpen Access
    PURIFICATION, ANTISERA PRODUCTION AND DEVELOPMENT OF DIAGNOSTIC KIT AGAINST POTATO VIRUS Y
    (AAU, Jorhat, 2018-07) Mishra, Ranima; Nath, P. D.
    Purified viral protein of Potato virus Y (PVY) was isolated from PVY culture maintained on potato (Solanum tuberosum L.) plants. The average concentration of the purified protein was found to be 153.1 ng/ μl with an average yield of 0.449 mg virus per gram of fresh plant tissue. Presence of flexuous filamentous virus particles with an average length of 590 nm in the purified viral suspension was confirmed by Transmission electron microscopy. PVY purified virus preparation was used for immunizing rabbit for production of polyclonal antisera. Good quality antisera were collected one week post boosters (AS4b, AS5b, AS6b and AS7b). The IgG fractions from these four antisera were tested for the detection of PVY by DAS-ELISA with universal anti- rabbit enzyme conjugate as secondary antibody, resulted high specificity with the known PVY infected samples. The assay was compared with the commercial DAS-ELISA kit (Bio Reba, AG, Switzerland). Among the antisera, AS6b was showing the highest mean absorbance value for all positive samples (2.210) which was at par the value shown by the commercial kit (2.250) and these were followed by AS5b (1.680), AS7b (0.929) and AS4b (0.362), respectively. AS6b was showing the highest mean absorbance values for the leaf extracts of samples which were statistically at par with the values shown by the commercial kit. IgG titres for the four batches were measured using a series of IgG dilutions from 10-3 to 10-6 with conjugate and sample dilutions at 10-3 and 100, respectively. Significant differences were observed in the titres of these four batches of IgG at 10-3 dilution. At that dilution, AS6b showed the highest mean absorbance value (1.272) followed by AS5b (1.009), AS7b (0.806) and AS4b (0.522), respectively. In DAS- ELISA with sap dilutions where IgG and conjugate dilutions were constant at 10-3, no significant differences observed in the mean absorbance values of all IgG batches at each sap dilutions and similar trend was observed, among the antisera batches, in sensitivity of IgG towards diluted samples. The AS6b showing the overall highest mean values followed by AS5b and AS7b, respectively. Finally, IgG batch 6b (AS6b) was selected and tested by a simple and rapid tissue/ dot- print immunoassay against PVY. The AS6b showed a highly specific reaction with dot- prints of PVY infected plants at IgG (AS6b) and IgG conjugate dilutions of 10-3. The test was done with sap dilutions of 1:1, 1:2, 1:4, 1:8, 1:16, 1:32 and 1:64 (V/V). AS6b could detect presence of PVY by showing the desired purple coloured reaction up to 1:8 sample dilution. Molecular characterization of PVY from PVY infected samples of Jorhat district, Assam was also carried out through reverse-transcriptase polymerase chain reaction (RT-PCR) assay resulting in desired 328 bp amplicon. Partial sequencing of RT-PCR product and phylogenetic analysis revealed that the virus is closely related to Potato virus Y worldwide isolates.