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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20211
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Subject: Plant Pathology × Author: Gogoi, Kulumanali × End date: 2021 × Start date: 2021 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    IDENTIFICATION, CHARACTERIZATION AND MANAGEMENT OF PUMPKIN MOSAIC DISEASE
    (AAU, Jorhat, 2021) Gogoi, Kulumanali; Mishra, Ranima
    The present investigation was conducted for identification, characterization and management of pumpkin mosaic disease in Assam. A roving survey was conducted during 2019-2021 in Jorhat, Golaghat, Karbi Anglong, Biswanath Chariali and Charaideo to determine the disease incidence through symptomatology, vector population count and detection of the viruses associated with mosaic disease of pumpkin through serodiagnostic (DAS-ELISA) and molecular assay (PCR and RT-PCR). Symptomatology revealed various degrees of mosaic, mottling, vein clearing, vein banding, chlorosis, leaf distortion, small, crinkled, deformed leaves and malformed fruits with mosaic and chlorotic spots resulting in reduced yield and production. Serological detection through DAS-ELISA confirmed the association of Cucumber mosaic virus (CMV) in CMV suspected pumpkin samples. The DAS-ELISA assay revealed disease incidence of 52.38 per cent in Golaghat district and 35.71 per cent in Jorhat district. Two different types of aphid vectors viz., Aphis gossypii and Myzus persicae were observed in all the surveyed locations. Total genomic RNA extracted from symptomatic and asymptomatic pumpkin plants were subjected to RT-PCR assays using CMV, zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus (WMV) specific primers and Deng universal primer for detection of begomovirus viz., pumpkin yellow vein mosaic virus (PYVMV). RT-PCR assay revealed the presence of CMV in the samples which yielded the desired amplicon size of 593 bp. No amplification was obtained from the samples for ZYMV and WMV. PCR product of CMV infected sample from Jorhat district viz., CMV-CP Jorhat isolate was sequenced and compared with the known CMV isolates worldwide using nucleotide BLAST programme at National Centre for Bio Informatics (NCBI) and Mega X software. The sequence similarity of CMV-CP Jorhat isolate ranged from 93.11 per cent to 99.16 per cent with worldwide CMV isolates. A field experiment was conducted for management of pumpkin mosaic disease through integrated approach. It was evident from the field experiment that there was low disease incidence with low vector population as well as high yield in the treatments of hot water seed treatment at 50⁰C for 10 minutes and spraying of neem formulation @3ml/lit at 15, 30, 45 and 60 days after transplanting (T1) followed by hot water seed treatment at 50⁰C for 10 minutes and spraying of Imidacloprid @2ml/lit at 15, 30, 45 and 60 days after transplanting (T6) than the other treatments with the highest yield (80.00 q/ha) recorded for the treatment T1 with highest per cent reduction of disease over control (53.22 per cent).