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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    ISOLATION, CHARACTERIZATION AND EVALUATION OF ENDOPHYTIC BACTERIA AGAINST ROOT-KNOT NEMATODES
    (AAU, Jorhat, 2020-09) Basumatary, Binita; Das, Debanand
    Fifteen endophytic bacteria were isolated from leaves and stems of Solanum lycopersicum and S pimpinellifolium. Morphological characterization of these endophytes revealed that 6 bacteria were gram positive and 9 bacteria were gram negative, 10 bacteria were rod shaped, 3 were coccus shaped and 2 were diplococcus in shape. All 15 bacteria were motile in nature. Biochemical characterization of the isolated endophytes revealed that 9 bacteria showed positive reaction to KOH test while, 6 were negative to KOH test, 11 bacteria showed positive reaction to Citrate and 4 were negative to citrate test. All the 15 bacteria were positive for Gelatine hydrolysis and Catalase test. Six endophytic bacteria showed positive reaction and 9 showed negative reactions for Starch hydrolysis test. Four potential isolates were structurally analysed by using scanning electron microscope and revealed that the length and breadth of BETLI, BETL2, BETL4 and BETS2 were 701.70nm × 348.30nm, 954.l0 nm x 303.10 nm, 984.10nm × 332.90nm and 1422.00nm × 742.00 nm, respectively. Five endophytic bacteria showing potentiality against root-knot nematode were identified by using 16S rRNA, and they were Microbacterium arborescens (BETLI), Bacillus marisflavi (BETL2), Bacillus altitudinis (BETL4), Exigobacterium indicum (BETS2) and Bacillus marisflavi (BETL6). Study on in vitro efficacy of bacterial endophytes against second stage juvenile of root-knot nematode (J2) revealed that all the isolates had the potentiality to significantly increase the mortality of nematodes however, the isolate BETL2 showed the best result on mortality rate (81.47%) of 2 stage juvenile (J2) of Meloidogyne incognita, followed by isolates BETL4 (81.43%) and BETLI (79.07%) Dose-response models were used to determine the concentration of bacterial culture filtrates required to kill 50 per cent juveniles of M. incognita. Four potential bacterial endophytes were tested with different methods of applications (seed treatment, seedling root dip treatment, soil application and their combinations) in pot condition against root-knot nematodes in tomato. All the tested endophytic bacteria significantly increased the plant growth parameters of tomato and reduced the nematode multiplication than untreated control. However, maximum increase in plant growth parameters and decrease in number of galls, egg mass and nematode population in soil was observed in combined application of seed treatment + root dip treatment + soil treatment of Bacillus marisflavi (BETL2) @ 1x 10 cfu/ml of crop.