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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20221
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Subject: Livestock Product Technology × Author: Muktan, Priya × End date: 2022 × Start date: 2022 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Development of enzyme based chromogenic strips for detection of selected adulterants in milk
    (College of Veterinary Science, Assam Agricultural University, Khanapara Campus, 2022-09) Muktan, Priya; Raquib, Masuk
    The present investigation was carried out to develop an enzyme based chromogenic strip for detection of selected adulterant in milk. The experiment was carried out in the Department of Livestock Products Technology, All India Coordinated Research Project on Post Harvest Engineering and Technology and Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati- 781022. An enzyme based chromogenic test strip using Whatman filter paper grade 602 and Whatman filter paper No. 1 was developed for the detection of glucose, starch and urea in milk using glucose oxidase, amyloglucosidase and urease enzyme in the presence of potassium iodide for starch and glucose and phenol red for urea as an indicator. The activity of the test strip was validated in both raw and processed milk spiked with the respective adulterants. All the enzyme based test strips were able to detect 2mg/ml of glucose, starch and urea with definite ring formation within a specified time period. The response time for the detection of glucose, starch and urea in milk was noted at 31.22±0.014 and 30.00±0.05, 128.3±0.88 and 129±0.57 & 99 ±0.57 and 109.67 ±0.88 sec, respectively in Whatman filter paper grade 602 and Whatman filter paper No. 1 at a pH of 4.5, 4.5 and 8.0 and a chromogenic substrate concentration of 40, 50 and 1 mg/ml. The optical density of glucose, starch and urea was found to be almost linear. As the concentration of the substrate increase the optical density value tends to increase proportionately. The test strip was tested for true positive and true negative results. The limit of detection for glucose, starch and urea were found to be 1.0, 2.0 and 0.8 mg/ml, with response time of 1, 4 and 3 min, respectively. To see the effect of different processing condition on the ability to degrade glucose, starch and urea in milk, all the test strips showed positive response except in household boiling condition wherein the response was delayed by a few min. The enzyme based test strip were 100 percent specific for detection of glucose, starch and urea as presence of similar types of compounds did not interfere with the positivity rate of the strips which was verified with help of confusion matrix. The shelf life of enzyme based chromogenic glucose, starch and urea test strips were found to be 40 and 30, 34 and 30 & 42 and 34 d, respectively for Whatman filter paper grade 602 and Whatman filter paper No. 1, respectively when stored under refrigerated storage (7±1oC) and ambient storage (29-32oC) condition in airtight glass containers.