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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    INFLUENCE OF ADDITIVES ON IN-VITRO MATURATION OF BOVINE OOCYTE
    (Assam Agricultural University, Khanapara, Guwahati, 2016-07) BHAJONI, MADHURIMA; Bhuyan, D.
    A study was conducted to find an effective in-vitro culture system based on in-vitro maturation of bovine oocytes. Ovaries from slaughter house were utilized to study ovarian biometry, follicular biometry and performance of in-vitro maturation of oocyte. Significantly higher weight, length, width and thickness were recorded in ovary with CL than that without CL. The number of large, medium and small follicles was more in ovary without CL than with CL group. The mean number of medium size follicles was significantly (P<0.01) higher in ovary without CL (6.32±0.75) than with CL (3.33±0.18). The recovery rates of grade A (47.58%) and B (37.42%) oocytes were higher than that of grade C (8.82%) and D (6.12%) by aspiration method. In the present study in-vitro maturation of oocytes was done at 38.5 0C in humidified atmosphere of 5% CO2 for 24 hours and matured in-vitro in medium-I or control (TCM-199+10% FBS+L-glutamine+sodium pyruvate+Gentamicin+pFSH+ Estradiol 17-β), medium-II (control+5% ECS), medium-III (control+100µM/ml cysteamine), medium-IV (control+10ng/ml EGF) and medium-V (control+ 5% ECS+ 100µM/ml cysteamine+10ng/ml EGF). The mean diameter of oocytes with cumulus cells for grade A oocytes varied significantly (P<0.01) after in-vitro maturation (IVM) in different media. The medium having either epidermal growth factor or cysteamine as additives showed higher diameter of oocytes after IVM as compared to medium with estrous cow serum or foetal bovine serum or combination of all three additives. The mean diameter of oocyte without cumulus cells before and after IVM did not differ significantly between different media. The increase in diameter of oocytes with cumulus cell for grade A was significantly (P˂0.05) higher in medium-III than that of I, II and V and in medium-IV than that of I, II and V. There was no significant difference in increase in diameter of oocyte without cumulus cells for grade A and oocyte with and without cumulus cells for grade B between different media. The rates of maturation based on cumulus cell expansion and nuclear maturation were the highest in the medium-IV (86.92% and 62.36%) containing epidermal growth factor (EGF) followed by medium-III (82.24% and 56.82%) containing cysteamine among all the media, the difference between media III and IV being non-significant.