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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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  • ThesisItemOpen Access
    CHARACTERIZATION AND QUALITY EVALUATION OF PIG FIBROBLAST CELLS AT DIFFERENT SUBCULTURES
    (Assam Agricultural University, Khanapara,Guwahati, 2017-07) DHAR, PRIYA; Nath, N. Ch.
    Fibroblast cells are the most common cells of connective tissue. In the present study, porcine fibroblast cells were isolated, cultured and subcultured upto six passages from adult and fetal skin samples. The time required to attain 70% confluence in primary culture of fetal fibroblast cells was found to be 26.40±2.40 hours, which was significantly lower (p≤0.01) than 67±4.80 hours of adult fibroblasts culture. The cells were maintained upto six passages which was 38.8 days for adult and 26 days for fetal fibroblast cells. The fetal fibroblast cells (days) required significantly lower (p≤0.01) time to subculture than adult fibroblast cells. Morphologically the isolated cells had fibroblastic character like typical fusiform shape, turgor vitalis cytoplasm, centrally located nuclei and flame like migration pattern upto sixth passage. The subculture time and cell viability (%) was significantly (p≤0.01) better from third subculture onwards upto sixth subculture as compare to primary and secondary cultures. TUNEL assay revealed a decreasing trend of apoptotic index from primary culture onwards to sixth subculture for both adult and fetal fibroblast cells. Fetal fibroblast cells maintained a lower apoptotic index than adult fibroblast in each of the subcultures. Therefore, fibroblast cells were found to be better from third subculture onwards to sixth subculture in regards to subculture time, viability (%), apoptotic index and in maintaining its normal morphological characteristics. ALP is considered as important marker for identification of pluripotent embryonic stem cells as well as multipotent mesenchymal stem cells. Isolated fibroblast cells of all the subcultures showed activity for ALP test indicating their progressive and undifferentiated quality and may be of multipotent in nature.