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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20211
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Subject: Animal Biochemistry × Author: HAZARIKA, DIPANKAR × Start date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    TOXICOLOGICAL ANALYSIS OF NANOPARTICLES AND MICROPARTICLES USED AS ORAL VACCINE DELIVERY SYSTEMS FOR POULTRY
    (College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2021-08) HAZARIKA, DIPANKAR; Tamuly, Shantanu
    Nanoparticles and microparticles offer great applications in the field of biological sciences in terms of oral drug and vaccine delivery systems. The present study was carried out to evaluate in-vitro and in-vivo toxicity associated with chitosan nanoparticles, Gantrez® nanoparticles and poly-lactide co-glycolide (PLG) microparticle in Vero cell line and poultry bird model. The Gantrez® nanoparticles, PLG microparticles and chitosan nanoparticles were administered orally to the week old poultry birds at the limit dose of 2 g per kg body weight for assessment of oral acute toxicity and were found to be safe as the birds did not show any mortality in 24 hours post administration and the birds did not show any clinical signs till 14 days post administration. For assessment of subacute toxicity, the chitosan nanoparticles, PLG microparticles and Gantrez® nanoparticles were administered at the dose rate used in vaccine delivery that is 3 mg/kg, 15 mg/kg and 1.5 mg/kg respectively and in the dose rate 10 times of the former. The second dose was administered after 14 days. No significant elevation of serum AST, ALT, ALP, BUN and creatinine were observed in the treated groups. In addition, the significant influence of the chitosan nanoparticles, PLG microparticles, and Gantrez® nanoparticles on elevation of blood SOD, GPx and catalase were not observed. The significant upregulation of HSP70 gene expression was observed in the spleen of the group treated with PLG microparticle at the dose rate of 150 mg/kg and in the liver of the group treated with Gantrez® nanoparticle at the dose rate of 15 mg/kg. On histopathological investigation, mild changes of congestion and haemorrhage was observed in kidney of the group treated with PLG microparticles at the dose rate of 150 mg/kg body wt. while in case of liver focal aggregation of mononuclear cell was observed in Gantrez® nanoparticles at the dose rate of 15 mg/kg body weight treated group. The congested capillaries in spleen were observed in the group treated PLG microparticles at the dose rate of 150 mg/kg body weight. The groups treated with vaccine dose of chitosan nanoparticles, Gantrez® nanoparticles and PLG microparticles have shown normal cellular architecture. In in vitro study in Vero cell line, the concentration of chitosan nanoparticles and Gantrez® nanoparticles up to 1000 μg/ml did not have any influence in cellular metabolic activities and viability. However, a reduction in the cellular viability and metabolic activities were observed when PLG microparticles were used at 1000 μg/ml. At lower concentrations, all the nanoparticles/microparticles were found to be safe in terms of cytotoxicity.