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Assam Agricultural University, Jorhat

Assam Agricultural University is the first institution of its kind in the whole of North-Eastern Region of India. The main goal of this institution is to produce globally competitive human resources in farm sectorand to carry out research in both conventional and frontier areas for production optimization as well as to disseminate the generated technologies as public good for benefitting the food growers/produces and traders involved in the sector while emphasizing on sustainability, equity and overall food security at household level. Genesis of AAU - The embryo of the agricultural research in the state of Assam was formed as early as 1897 with the establishment of the Upper Shillong Experimental Farm (now in Meghalaya) just after about a decade of creation of the agricultural department in 1882. However, the seeds of agricultural research in today’s Assam were sown in the dawn of the twentieth century with the establishment of two Rice Experimental Stations, one at Karimganj in Barak valley in 1913 and the other at Titabor in Brahmaputra valley in 1923. Subsequent to these research stations, a number of research stations were established to conduct research on important crops, more specifically, jute, pulses, oilseeds etc. The Assam Agricultural University was established on April 1, 1969 under The Assam Agricultural University Act, 1968’ with the mandate of imparting farm education, conduct research in agriculture and allied sciences and to effectively disseminate technologies so generated. Before establishment of the University, there were altogether 17 research schemes/projects in the state under the Department of Agriculture. By July 1973, all the research projects and 10 experimental farms were transferred by the Government of Assam to the AAU which already inherited the College of Agriculture and its farm at Barbheta, Jorhat and College of Veterinary Sciences at Khanapara, Guwahati. Subsequently, College of Community Science at Jorhat (1969), College of Fisheries at Raha (1988), Biswanath College of Agriculture at Biswanath Chariali (1988) and Lakhimpur College of Veterinary Science at Joyhing, North Lakhimpur (1988) were established. Presently, the University has three more colleges under its jurisdiction, viz., Sarat Chandra Singha College of Agriculture, Chapar, College of Horticulture, Nalbari & College of Sericulture, Titabar. Similarly, few more regional research stations at Shillongani, Diphu, Gossaigaon, Lakhimpur; and commodity research stations at Kahikuchi, Buralikson, Tinsukia, Kharua, Burnihat and Mandira were added to generate location and crop specific agricultural production packages.

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20181
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Subject: Agricultural Biotechnology × Author: Hazarika, Geetimollika × End date: 2018 × Start date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF NBS-LRR RESISTANT GENE ANALOGUES (RGAs) FROM INDIGENOUS AND WILD BANANA (MUSA SPP.) GERMPLASM
    (AAU, Jorhat, 2018-01) Hazarika, Geetimollika; Bhorali, Priyadarshini
    Commercial banana varieties are highly susceptible to fungal and bacterial pathogens, nematodes, viruses and insect pests. The largest known family of plant disease resistance (R) genes encodes proteins with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense. In the present study, an attempt was made to isolate and characterize the conserved region NBS of the NBS-LRR resistance gene analogues (RGAs) from locally cultivated indigenous and wild banana germplasms of Assam. The investigation was started with the isolation of genomic DNA from ten cultivated indigenous germplasms viz. Kach kol, Cheni champa, Ukho jahaji, Malbhog, Manuhor, Athiya kol, Bhim kol, Ketekihunda, Phesa manuhor and Ximolu manuhor and, five wild germplasms (designated as W1, W2, W3, W4 and W5). To target the NBS region of the banana germplasms, four pairs of PCR primers out of which two were degenerate primers, were designed from existing NBS-LRR sequences available in the GenBank. After successful isolation and sequencing of the PCR amplified NBS fragments from all the fifteen samples, confirmation about the identity of the sequences was done by homology search using BLASTn and BLASTp algorithms which revealed the sequences to be significantly similar to the NBS-LRR class disease resistance proteins available in NCBI. The sequence identity was further confirmed by checking for the Pfam NB-ARC domain, which is a protein domain characteristic of the plant resistance NBS-LRR protein. The NB-ARC domain was obtained in all the isolated NBS sequences. Finally, the presence of the consensus sequence for Kinase-2 motif (LLDDVW) and phylogenetic analysis of the isolated NBS sequences further provided evidence that the sequences belong to the typical non-Toll/interleukin-1 receptor- like domain NBS-LRR gene family, as expected. As a future prospect, upon cloning of the full length NBS-LRR sequences from these germplasms would open up possibilities for development of disease resistant cultivars through genetic engineering approaches.